immunology of infectious and parasitic diseases - XXXVII Congress ...

IMMUNODETECTION OF THE TGF- β IN LIVER OF DOGS WITH VISCERAL
LEISHMANIASIS
PAMELA RODRIGUES REINA MOREIRA 1 ; MARCIO DE BARROS
BANDARRA 1 ; DANÍSIO PRADO MUNARI 2 ; ROSEMERI DE OLIVEIRA
VASCONCELOS 3
(1). Doutorandos do Programa Pós-graduação em Medicina Veterinária. (2).
Depto. de Ciências Exatas; (3). Patologia Veterinária, FCAV/UNESP,
Jaboticabal-SP. rosevasc@fcav.unesp.br
Introduction:: In canine visceral leishmaniasis (CVL) reported the
involvement of various organs by Leishmania (Leishmania) chagasi and
consider liver an organ resistant multiplying it. The objective of this study
was to analyze liver injury in dogs with CVL and associate them with the
immunodetection of TGF-β and the parasite burden by means of
immunohistochemical technique in different clinical stages of disease,
such as symptomatic (S), asymptomatic (A) and oligosymptomatic (O).
Methods and Results: This study included 35 dogs, without preference for
age, breed or sex, from the Zoonosis Control Center of Araçatuba (SP),
where they were euthanized due to the positive diagnosis of visceral
leishmaniasis (VL). One control group (C) was formed with dogs from nonendemic
area for CVL (CEUA nº. 024686/10). In immunohistochemical analysis
we used the antibodies TGF-β (Santa Cruz Biotecnology) and for the parasite
burden was used canine hyperimmune serum (J. Immunol. Meth. 292:17-23,
2004). The antibodies were used at dilutions of 1:1300 and 1:1000 respectively.
The mean cells immunostained for Leishmania sp. and TGF-β were analyzed
by Kruskal-Wallis and the Dunn test to compare the groups, considering P