immunology of infectious and parasitic diseases - XXXVII Congress ...
immunology of infectious and parasitic diseases - XXXVII Congress ...
immunology of infectious and parasitic diseases - XXXVII Congress ...
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KILLER CELL IMMUNOGLOBULIN-LIKE RECEPTOR GENES IN PATIENTS<br />
WITH CHAGAS DISEASE<br />
CHRISTIANE MARIA AYO 1 ; PÂMELA GUIMARÃES REIS 1 ; CAMILA DE<br />
FREITAS OLIVEIRA 1 ; EMÍLIA ÂNGELA SIPPERT 1 ; MÁRCIA MACHADO DE<br />
OLIVEIRA DALÁLIO 1 ; JEANE ELIETE LAGUILA VISENTAINER 1 ; ANA MARIA<br />
SELL 1<br />
1 Universidade Estadual de Maringá<br />
Introduction: Chagas disease, caused by Trypanosoma cruzi, occurs<br />
throughout Latin America <strong>and</strong> is one <strong>of</strong> most serious <strong>parasitic</strong> <strong>diseases</strong> affecting<br />
millions <strong>of</strong> people. The disease is classified in acute <strong>and</strong> chronic phase but the<br />
clinical manifestations vary from one endemic area to another <strong>and</strong> this variation<br />
can be attributable to genetic factors. KIR genes (killer cell immunoglobulin-like<br />
receptor) encode molecules activating <strong>and</strong> inhibitory function <strong>of</strong> NK cells<br />
(natural killer) <strong>and</strong> have as lig<strong>and</strong>s HLA (human leukocyte antigen) class I.<br />
Both KIR <strong>and</strong> HLA molecules are highly polymorphic <strong>and</strong> the specific KIR-HLA<br />
allelic combinations may regulate NKcell-mediated immunity against <strong>infectious</strong><br />
pathogens. The aim <strong>of</strong> this study was to investigate the association <strong>of</strong> KIR<br />
genes with their lig<strong>and</strong>s HLA in chronic chagasic patients <strong>and</strong> controls in a<br />
population from North/Northwest <strong>of</strong> Parana State, South <strong>of</strong> Brazil. Methods <strong>and</strong><br />
Results: A total <strong>of</strong> 50 patients <strong>and</strong> 65 controls were evaluated <strong>and</strong> all they<br />
were typed for 16 KIR genes <strong>and</strong> HLA class I alleles by rSSO technique (One<br />
Lambda Inc., USA).The observed gene frequencies were determined by direct<br />
counting <strong>and</strong> statistical analysis was performed using the Fisher exact test <strong>and</strong><br />
Chi Squares with Yates correction. Individual analysis <strong>of</strong> these KIR genes did<br />
not show significant correlation. However when analyzed the interation between<br />
KIR-HLA lig<strong>and</strong>s, KIR3DL2 - HLA-A3/11(p: 0.004; OR: 0.21; CI: 0.05-0.66) was<br />
significantly more frequent in the controls. A significant increased <strong>of</strong> the lig<strong>and</strong>s<br />
HLA-A3/11 also was observed in the controls group (p: 0.007; OR: 0.25; CI:<br />
0.07-0.73), when these lig<strong>and</strong>s were analyzed separately. Conclusions: We<br />
did not observe the influence <strong>of</strong> KIR genes <strong>and</strong> their HLA lig<strong>and</strong>s (-A,-B <strong>and</strong>-C)<br />
in the susceptibility to Chagas disease. As KIR3DL2 is a framework gene,<br />
present virtually all genotypes, significance in the control group may have been<br />
because <strong>of</strong> HLA-A03/11 frequencies only. More investigations will be done in<br />
order to underst<strong>and</strong> the role <strong>of</strong> KIR genes in Chagas disease.<br />
Financial support: Fundação Araucária.
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