immunology of infectious and parasitic diseases - XXXVII Congress ...

PROFILE OF PRO- AND ANTI-INFLAMMATORY CYTOKINES IN PATIENTS
WITH MALARIA ASSISTED AT THE INSTITUTO DE MEDICINA TROPICAL
OF COARI-AM
ALLYSON GUIMARÃES DA COSTA (PG)(1,2); JOÃO PAULO DINIZ
PIMENTEL (PG)(2,3); NADJA PINTO GARCIA (PG)(2); ANDRÉA MONTEIRO
TARRAGÔ (PG)(1,2); LUCYANE BASTOS TAVARES DA SILVA (IC)(2);
JÉSSICA PEREIRA MARQUES (IC)(2); WALTER LUIZ LIMA NEVES
(PG)(1,2); ADRIANA MALHEIRO (PG)(1,2).
(1). Universidade Federal do Amazonas; (2). Fundação de Hematologia e
Hemoterapia do Amazonas; (3). Instituto Leônidas e Maria Deane/Fiocruz
Amazônia.
Introduction: Malaria is a major parasitic disease of tropical regions. Three
species are associated with this infection in Brazil P. vivax, P. falciparum and P.
malariae. The immune response has many defense mechanisms which result in
the inflammation process and the individual resistance to the pathogen. In
general, the clinical manifestations are dependent on the concentrations of pro
and anti-inflammatory cytokines, which contribute to the immunopathology of
the disease. The aim of this study was to quantify the inflammatory and antiinflammatory
cytokines in individuals with malaria treated at the Instituto de
Medicina Tropical of Coari-AM. Methods and Results: Serum levels of
cytokines (IL-2, IL-4, IL-6, IL-10, TNF-, IFN- and IL-17) were measured in the
plasma of 50 individuals (25 malaria patients infected by Plasmodium vivax and
25 uninfected - control group) using the technique of CBA (Citometry Bead
Array) using the kit TH1, TH2 and TH17 (BD Biosciences). The analyzes of
serum concentrations were performed with the software FCAP-Array (v3.0) and
the results were analyzed using the statistical software GraphPad Prism ® (v5.0),
using the nonparametric Mann-Whitney test. The concentrations of IL-2, IL-4
and TNF- were lower among patients with malaria compared with control
subjects (p=0.1252, p=0.4707 and p=0.0456, respectively). While serum levels
of IL-6, IL-10, IL-17 and IFN- were significantly higher among the infected
group compared to uninfected individuals (p