immunology of infectious and parasitic diseases - XXXVII Congress ...

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UNDERSTANDING IMMUNE AND INFLAMMATORY RESPONSES DEVELOPED BY BALB/C MICE INFECTED WITH LEISHMANIA (VIANNIA) BRAZILIENSIS, A RESISTANT INFECTION MODEL FOR LEISHMANIASIS MÍRIAM CONCEIÇÃO DE SOUZA TESTASICCA (1) ; AMANDA BRAGA DE FIGUEIREDO (2) ; JULIANA PRISCILA VAGO (1) ; THAÍS ROLLA DE CAUX (1) ; SIMONE APARECIDA REZENDE (2) ; LUÍS CARLOS CROCCO AFONSO (2) ; LIRLÂNDIA PIRES DE SOUSA (1) ; ANA PAULA FERNANDES (1) (1) Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil; (2) Universidade Federal de Ouro Preto, Ouro Preto, MG, Brasil; Introduction: Human infection with L. braziliensis has been associated with a broad range of clinical manifestations, from simple single lesions to the mucosal form. However, there are fewer studies about infection with this parasite species in mice models in comparison with other species of this genus, which makes difficult the identification of the immune and inflammatory mechanisms associated to infection. Studies characterizing the interaction between these parasites and their hosts can expand the understanding of the pathology associated with infection and potentially identify new biomarkers for resistant and susceptibility profiles of infection that could be applicable to vaccine and chemotherapy studies. Thus, the aim of this work was to characterize the kinetics of the immune and inflammatory responses developed by BALB/c mice after experimental infection with L. braziliensis. Methods and Results: Our results show that L. braziliensis infection of BALB/c mice causes a lesion that is self-limiting, in which parasites are found at initial time of infection (2 weeks) and declined thereafter (analyzed by limiting dilution assay) as expected for a resistant infection profile. Splenocytes of the infected animals respond to the restimulation with specific antigen producing IFN-γ. In our model, the mice did not develop T multifunctional cells and memory CD4 + CCR7 + CD62L + in response to infection. Analysis of signaling pathways involved in inflammatory responses showed an increase of ERK1/2 phosphorylation at initial stages of infection that decreased at the times of resolution of the lesion. Interestingly, it was observed an increase in the anti-inflammatory protein annexin A1 in the initial stages post-infection. Conclusion: Together, these results confirm that infection of BALB/c mice by L. braziliensis is an infection resistance model and let us formulate strategies for better characterize the inflammatory response. Further investigations of the immune and inflammatory profile caused by L. braziliensis compared to those induced by other species may permit a better understanding of the role of inflammatory signaling pathways in leishmaniasis and its possible role in the prognosis of the disease development. Financial Support: INCTV, CNPq, FAPEMIG.
ROLE OF ADENOSINE AND PROSTAGLANDIN E2 ON TICK INFESTATION MICHELLE CHRISTIANE RODRIGUES BARBOSA 1 ; CARLO JOSÉ FREIRE DE OLIVEIRA 2 ; ELEN ANATRIELLO 1 ; LAUREN CRISTINA DA SILVA RIBEIRO 1 ; ISABEL KINNEY FERREIRA DE MIRANDA SANTOS 1 ; BEATRIZ ROSSETTI FERREIRA 3 . 1 School of Medicine of Ribeirão Preto, USP-SP 2 Federal University of Triângulo Mineiro, UFTM, Uberaba-MG 3 School of Nursing of Ribeirão Preto, USP-SP Introduction: Saliva of ticks contains several components with anti-haemostatic, anti-inflammatory and immunomodullatory properties. Our group recently demonstrated that Rhipicephalus sanguineus tick saliva contains adenosine (ADO) and prostaglandin E2 (PGE2), two non-protein molecules that have important immunomodullatory properties, including cytokine production. PGE2 acts on the immune response cells via receptor EP1, EP2, EP3, and EP4, as ADO via receptors A1, A3, A2a and A2b. Objectives: Evaluate the importance of ADO and PGE2 on tick-infested mice in vivo. Methods and Results: C57/Bl6 mice were submitted to a R. sanguineus tick infestation with adult ticks (3 couples/mice) and in the 1 st , 4 th and 7 th day we collected the skin at the tick-infested site. Subsequently, we evaluated the expression of genes for the receptors of ADO and PGE2, as well as IL-4 and IL-13 using the real time PCR assay. Tick infestation increased in 5 times (p
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IMMUNOLOGY OF INFECTIOUS AND PARASI
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profiles appear to be dependent on
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INVOLVEMENT OF TNF RECEPTORS IN APO
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Effects of bioactive Cryptococcus n
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DCs expressing Indoleamine 2,3-diox
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DETECTION OF GITR ON PATIENT CERVIX
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DETECTION OF GITR AND IL-10 ON CERV
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VACCINATION WITH SM10.3 ANTIGEN, A
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Financial support: FAPESP, CAPES an
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clearance that follows Sylvio X10/4
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EhMSP-1 - AN ENTAMOEBA HISTOLYTICA
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THERAPY WITH SCFV TRANSFECTED-DENDR
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etween the groups. The results show
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Financial support: FIOCRUZ, CNPq.
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elease of NETs from human neutrophi
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ROLE OF CD18 IN ACTIVATION OF M1 MA
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IDENTIFICATION, PURIFICATION AND CH
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with hemin showed higher levels of
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SEPTIC ARTHRITIS TRIGGERED BY S. au
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eing higher levels induced by a vir
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parasite load skin. The TGF-β was
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cultures, both treatments decreased
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observed a tendency to lower parasi
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SERUM COMPONENTS AND MONONUCLEAR CE
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VIABILITY OF LEISHMANIA (L.) CHAGAS
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EVALUATION OF THE INTERFERENCE OF S
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IMMUNODETECTION OF THE TGF- β IN L
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DIFFERENT LEVELS OF NKT AND NK CELL
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Funding: CNPQ; FAPESPA; SESPA; UFPA
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saliva that exacerbated leishmania
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BACTERIAL FILAMENTATION: SUPER-RESI
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IMMUNOLOGICAL PARAMETERS ASSOCIATED
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supernatant. BMDC stimulation with
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such as number of eosinophils, prot
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EVALUATION OF THE PRODUCTION OF TNF
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Critical motifs of the Anaplasma ma
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SCHISTOSOMA SPP ANTIGENS IMPAIR THE
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DOWN-REGULATION OF IFN-g RECEPTOR A
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MONOCYTES SUBSETS IN SCHISTOSOMIASI
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THE IN VITRO ANALYSIS OF iNOS-KO MO
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CCR7 IS CRITICAL FOR RESISTANCE AGA
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EVALUATION OF THE IMMUNE RESPONSE A
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our data suggest that monocyte secr
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DIVERGENT OUTCOMES OF THE INTERACTI
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they can deactivate immune effector
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Conclusions: We observed a signific
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TLR2 AND TLR4 EXPRESSION AND NUTRIT
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DEVELOPMENT OF MURINE PLACENTAL MAL
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INDUCTION OF TH17 LYMPHOCYTES CONTR
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Leishmania amazonensis INCREASES EC
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TYPE II GRANULOMA INDUCED BY SCHIST
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The Role of Eosinophils in Aspergil
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compared to baseline (p
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disordered loop and NcSAG2A present
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Conclusion: We demonstrate the exis
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WT. By contrast, uninfected AhR -/-
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in the synthesis of proinflammatory
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PRODUCTION AND EVALUATION OF CHICKE
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allow a conclusion about the differ
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contributes to parasite control and
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Financial support: FAPESP, CNPq, an
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MILTEFOSINE EXERTS ITS LEISHMANICID
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IMMUNOMODULATORY ACTIVITIES OF Β-G
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REGULATION OF IMMUNE RESPONSE AGAIN
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THE ROLE OF REACTIVE OXIGEN SPECIES
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INNATE AND ADAPTIVE IMMUNE REPONSE
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KINETIC OF THE SPECIFIC HUMMORAL IM
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PURINERGIC RECEPTOR P2Y12 ACTIVATIO
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Evaluation of cytokine and chemokin
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Financial support: This research wa
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COMPARISON OF VIRULENCE AND IMMUNE
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THE IMPACT OF TUBERCULOSIS IN THE I
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GALACTOMANNAN ANTIGENEMIA SCREENING
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NATURAL ISOTYPIC RESPONSE AGAINST P
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Characterization of costimulatory m
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P2X7 RECEPTOR ACTIVATION IS REQUIRE
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CASPASE-1 AND NLRP3 CONTRIBUTE TO T
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circulating Treg cells expressing C
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PBMC, none of the treatments alter
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CHARACTERIZATION OF MONOCYTE SUBSET
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EFFECT OF OUABAIN IN MACROPHAGES IN
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OspC1 AND OspF: VIRULENCE FACTORS I
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Conclusion: Taken together, these r
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FIB produced higher proportion of f
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MØ phenotype M2, resulting in decr
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Financial support FAPESP, CAPES, CN
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ALTERATION IN THE DISTRIBUTION OF P
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BENEFICIAL EFFECTS OF PENTOXIFYLLIN
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MODULATION OF IMMUNE RESPONSE AND C
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FAS/FASL AND TRAIL CAUSE APOPTOSIS
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actin were more frequently detected
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NEUTROPHILS AND MACROPHAGES ARE INV
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EXPLORING THE INFLAMMATORY ROLE OF
Page 187 and 188: CERVICAL LEVELS OF INTERLEUKIN-1 BE
Page 189 and 190: Title: IDENTIFICATION BY PHAGE DISP
Page 191 and 192: PROFILE OF PRO- AND ANTI-INFLAMMATO
Page 193 and 194: IMMUNOMODULATORY MECHANISMS OF SOLU
Page 195 and 196: EVALUATION OF THE TH1, TH2 AND TH17
Page 197 and 198: production, as the blockade of PD-L
Page 199 and 200: CHARACTERIZATION OF POLYMORPHISM TN
Page 201 and 202: seems to be dependent on M. leprae
Page 203 and 204: 7 with tendency to normalization on
Page 205 and 206: CHARACTERIZATION OF THE CONSERVATIO
Page 207 and 208: ASC INFLAMMASOME IS NECESSARY TO AC
Page 209 and 210: CASPASE-1 IS REQUIRED TO CONTROL OF
Page 211 and 212: The role of CD4 + T lymphocytes dur
Page 213 and 214: CRITICAL ROLE OF MYD88 EXPRESSION I
Page 215 and 216: on ROS, cathepsin B and Syk kinase
Page 217 and 218: Financial Support: FAPESP and CNPq.
Page 219 and 220: 80% in symptomatic group; 45% in as
Page 221 and 222: of cells undergoing apoptosis in DT
Page 223 and 224: Financial support: FAPESP/LIM-56/HC
Page 225 and 226: identify the active components and
Page 227 and 228: EVALUATION OF HUMORAL AND CELULLAR
Page 229 and 230: Financial support: CNPq-PIBIC, FAPE
Page 231 and 232: addition, significant expression of
Page 233 and 234: THE ROLE OF K + TRANSPORTERS IN THE
Page 235 and 236: Conclusion: In this study, we estab
Page 237: Heme activates oxidative mechanisms
Page 241 and 242: Taken together, our results indicat
Page 243 and 244: Conclusion: Our findings suggest th
Page 245 and 246: Financial support: CNPq, CAPES, IOC
Page 247 and 248: number of circulating parasites in
Page 249 and 250: CRITICAL ROLE OF IL-6 IN REGULATORY
Page 251 and 252: PPAR-α AGONIST GEMFIBROZIL DECREAS
Page 253 and 254: Cutaneous Leishmaniasis in Amazonas
Page 255 and 256: CYTOTOXICITY IN IMMUNOPATHOGENESIS
Page 257 and 258: ROS production and TLR2 and 4 expre
Page 259 and 260: ROLE OF IL-4 IN THE INTESTINAL INFL
Page 261 and 262: In vitro CULTURE SYSTEM FOR Plasmod
Page 263 and 264: interactions, Tandem Affinity Purif
Page 265 and 266: in the course of human infection wi
Page 267 and 268: findings suggest that IL-17 and IL-
Page 269 and 270: 23 contribute to immunological cont
Page 271 and 272: not produced during infection by C.
Page 273 and 274: Conclusion: The production of cytok
Page 275 and 276: CONCLUSION: It is likely, in this p
Page 277 and 278: expression in CCC myocardium was 64
Page 279 and 280: that mononuclear cells derived from
Page 281 and 282: Conclusion: Altogether, our data sh
Page 283 and 284: PARACOCCIDIOIDES BRASILIENSIS INHIB
Page 285 and 286: CANINE VISCERAL LEISHMANIASIS AND S
Page 287 and 288: IMMUNOMODULATORY EFFECTS OF BIOTHER
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Leishmania EFFECT ON HEME CYTOTOXIC
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ROLE OF STRONGYLOIDES VENEZUELENSIS
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TREATMENT WITH Harpagophytum procum
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