immunology of infectious and parasitic diseases - XXXVII Congress ...

Title: PRO-INFLAMMATORY CYTOKINES PRODUCTION IN PATIENTS
INFECTED WITH DIFFERENT CSP MOLECULAR VARIANTS OF
Plasmodium vivax
Authors: BRUNO DE PAULO RIBEIRO 1 ; ANA PAULA SILVA DE AZEVEDO
DOS SANTOS 1 ; DALILA NUNES CYSNE 1 ; GUILHERME TUDE COELHO
NETO 1 ; ERICKA MIRANDA MESQUITA 2 ; GUSTAVO CAPATTI CASSIANO 3 ;
ELOÍSA DA GRAÇA DO ROSÁRIO GONÇALVES 4 ; MARCOS AUGUSTO
GRIGOLIN GRISOTTO 2 ; RICARDO LUIZ DANTAS MACHADO 3 ; FLÁVIA
RAQUEL FERNANDES DO NASCIMENTO 1
1 Laboratório de Imunofisiologia - Universidade Federal do Maranhão (UFMA) –
São Luís, Brazil; 2 Laboratório de Imunologia das Parasitoses - Universidade
CEUMA (UniCEUMA) – São Luís, Brazil; 3 Centro de Investigação de
Microrganismos - Faculdade de Medicina de São José do Rio Preto (FAMERP)
– São José do Rio Preto, Brazil. 4 Centro de Referência em Doenças Infectoparasitárias
- Universidade Federal do Maranhão (UFMA) – São Luís, Brazil.
Introduction: P.vivax causes the majority of human malaria cases worldwide,
despite this it remains largely neglected. Vivax malaria was thought to be a
benign and self-limited disease for a long time; currently, complications
associated to this disease in the Amazon region and other places throughout
the world have motivated research of the related immunopathological events.
One of the many possible causes for complications may be the genetic
characteristics of the species.Taking in consideration that pathological changes
in malaria, which determine either alleviation or exacerbation of the disease,
also depend of a delicate balance in cytokines milieu, we assessed the
production of pro-inflammatory cytokines according to the molecular variants of
P. vivax Circunsporozoit Protein (CSP) -VK210 and VK247.
Methods and Results: Blood samples from untreated vivax malaria patients
(n=28) were used to provide the plasma and red blood cells containing
parasites. Pro-inflammatory cytokines levels (IFN-g, IL-6 and TNF-a) in the
plasma were assessed through Cytometric Bead Array (CBA) and the vivax
CSP molecular variants were characterized by RFLP-PCR. The values were
described in means ± SEM. There was a tendence of higher IL-6 and IFN-g in
VK210 patients (IL-6: VK210 217±152 and VK247 34.9±22; IFN-g: VK210
8.5±6.7 and VK247 0.31±0.31), but there were no statistical differences. TNF-a
and P.vivax-like variant was not detectable in patients.
Conclusion: CSP vivax variants VK210 and VK247do not influence the
expression of pro-inflammatory cytokines in humans.
Financial support: FAPEMA; CNPq; CAPES.