immunology of infectious and parasitic diseases - XXXVII Congress ...

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AUTHENTIC LTB4 PRODUCED BY Paracoccidioides brasiliensis: EFFECT ON IMMUNOMODULATORY ACTIVITY OF HUMAN POLYMORPHONUCLEAR AND MONONUCLEAR CELLS Ana Paula Bordon-Graciani (1), Guilherme Augusto Biondo (PG) (1), Luciane Alarcão Dias-Melicio (2), Tatiana Fernanda Bachiega (PG) (1), Marjorie de Assis Golim (3), José Roberto Marques Silva (4), Ângela Maria Victoriano de Campos Soares (1). (1) Departamento de Microbiologia e Imunologia, Instituto de Biociências de Botucatu, UNESP – Univ Estadual Paulista, Brasil; (2) Departamento de Patologia, Faculdade de Medicina de Botucatu, UNESP – Univ Estadual Paulista, Brasil; (3) Hemocentro, Faculdade de Medicina de Botucatu, UNESP – Univ Estadual Paulista, Brasil; (4) Núcleo de Pesquisa Avançada em Matologia, Faculdade de Ciências Agronômicas de Botucatu, UNESP – Univ Estadual Paulista, Brasil. Introduction: Paracoccidioides brasiliensis is the agent of paracoccidioidomycosis, the most prevalent deep mycosis in Latin America. The production of eicosanoids during fungal infection has been associated with the biology of fungi, and the modulation of host immune response. Leukotrienes (LTs) are proinflammatory mediators produced by various mammalian cells. Studies in our laboratory have shown that, in addition of host cells, another source of LTB4 production is P. brasiliensis itself. The objective of this study was to assess the effect of LTB4 produced by P. brasiliensis on effector functions of human polymorphonuclear and mononuclear cells. Methods and Results: Peripheral blood neutrophils (PMN), monocytes (MO) and PBMC obtained from 10 healthy donors were treated with LTB4 purified from P. brasiliensis yeast cells cultures (LTB4f) or commercial LTB4c (LTB4c). After 1, 2, 4, 8 and 12 hours of incubation, PMN and MO cultures were evaluated by phagocytosis assay, surface receptors expression (TLR-2, TLR4, dectin-1, MR and HLA-DR), H2O2, cytokines (TNF-α, IL-6, IL-8, IL-10, IL-12p70, IL-15, IL-17, IL-23, IL-27) and chemokines (MCP-1 and MIP-1α) production. PBMC cultures were evaluated after 24, 48 and 72 hours of incubation with PGE2f and PGE2c by cellular proliferation and cytokines production (TNF-α, IFN-, IL-2, IL-4, IL-10, IL-12 and IL-17). The treatment with either LTB4c or LTB4f increased phagocytosis of zymosan and H2O2 production after estimulation with PMA, in both PMN and MO cultures.The treatment also increased TLR-2 expression on PMN surface and MR expression on MO. In relation to cytokines and chemokines produced by PMNs there was an increase on TNF-α, IL-6, IL-8 and MCP-1 production. MO showed an increase on TNF-α, IL-6, IL-12, IL-15, IL-17, IL-23 and MCP-1, and a decrease on IL-8 and IL-10 production. Regarding
PBMC, none of the treatments alter cell proliferation,but increased TNF-α, IFN- , IL-2 and IL-17 levels and decreased IL-4 and IL-10 production. Conclusion: Our results show that LTB4 purified from P. brasiliensis cultures modulate human innate and adaptive response similarly as LTB4c does. Financial support: FAPESP (Process n°:2009/13751-9; Process n°: 2009/08938-2).
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IMMUNOLOGY OF INFECTIOUS AND PARASI
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profiles appear to be dependent on
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INVOLVEMENT OF TNF RECEPTORS IN APO
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Effects of bioactive Cryptococcus n
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DCs expressing Indoleamine 2,3-diox
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DETECTION OF GITR ON PATIENT CERVIX
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DETECTION OF GITR AND IL-10 ON CERV
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VACCINATION WITH SM10.3 ANTIGEN, A
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Financial support: FAPESP, CAPES an
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clearance that follows Sylvio X10/4
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EhMSP-1 - AN ENTAMOEBA HISTOLYTICA
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THERAPY WITH SCFV TRANSFECTED-DENDR
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etween the groups. The results show
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Financial support: FIOCRUZ, CNPq.
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elease of NETs from human neutrophi
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ROLE OF CD18 IN ACTIVATION OF M1 MA
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IDENTIFICATION, PURIFICATION AND CH
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with hemin showed higher levels of
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SEPTIC ARTHRITIS TRIGGERED BY S. au
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eing higher levels induced by a vir
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parasite load skin. The TGF-β was
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cultures, both treatments decreased
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observed a tendency to lower parasi
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SERUM COMPONENTS AND MONONUCLEAR CE
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VIABILITY OF LEISHMANIA (L.) CHAGAS
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EVALUATION OF THE INTERFERENCE OF S
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IMMUNODETECTION OF THE TGF- β IN L
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DIFFERENT LEVELS OF NKT AND NK CELL
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Funding: CNPQ; FAPESPA; SESPA; UFPA
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saliva that exacerbated leishmania
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BACTERIAL FILAMENTATION: SUPER-RESI
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IMMUNOLOGICAL PARAMETERS ASSOCIATED
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supernatant. BMDC stimulation with
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such as number of eosinophils, prot
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EVALUATION OF THE PRODUCTION OF TNF
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Critical motifs of the Anaplasma ma
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SCHISTOSOMA SPP ANTIGENS IMPAIR THE
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DOWN-REGULATION OF IFN-g RECEPTOR A
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MONOCYTES SUBSETS IN SCHISTOSOMIASI
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THE IN VITRO ANALYSIS OF iNOS-KO MO
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CCR7 IS CRITICAL FOR RESISTANCE AGA
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EVALUATION OF THE IMMUNE RESPONSE A
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our data suggest that monocyte secr
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DIVERGENT OUTCOMES OF THE INTERACTI
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they can deactivate immune effector
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Conclusions: We observed a signific
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TLR2 AND TLR4 EXPRESSION AND NUTRIT
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DEVELOPMENT OF MURINE PLACENTAL MAL
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INDUCTION OF TH17 LYMPHOCYTES CONTR
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Leishmania amazonensis INCREASES EC
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TYPE II GRANULOMA INDUCED BY SCHIST
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The Role of Eosinophils in Aspergil
Page 105 and 106: compared to baseline (p
Page 107 and 108: disordered loop and NcSAG2A present
Page 109 and 110: Conclusion: We demonstrate the exis
Page 111 and 112: WT. By contrast, uninfected AhR -/-
Page 113 and 114: in the synthesis of proinflammatory
Page 115 and 116: PRODUCTION AND EVALUATION OF CHICKE
Page 117 and 118: allow a conclusion about the differ
Page 119 and 120: contributes to parasite control and
Page 121 and 122: Financial support: FAPESP, CNPq, an
Page 123 and 124: MILTEFOSINE EXERTS ITS LEISHMANICID
Page 125 and 126: IMMUNOMODULATORY ACTIVITIES OF Β-G
Page 127 and 128: REGULATION OF IMMUNE RESPONSE AGAIN
Page 129 and 130: THE ROLE OF REACTIVE OXIGEN SPECIES
Page 131 and 132: INNATE AND ADAPTIVE IMMUNE REPONSE
Page 133 and 134: KINETIC OF THE SPECIFIC HUMMORAL IM
Page 135 and 136: PURINERGIC RECEPTOR P2Y12 ACTIVATIO
Page 137 and 138: Evaluation of cytokine and chemokin
Page 139 and 140: Financial support: This research wa
Page 141 and 142: COMPARISON OF VIRULENCE AND IMMUNE
Page 143 and 144: THE IMPACT OF TUBERCULOSIS IN THE I
Page 145 and 146: GALACTOMANNAN ANTIGENEMIA SCREENING
Page 147 and 148: NATURAL ISOTYPIC RESPONSE AGAINST P
Page 149 and 150: Characterization of costimulatory m
Page 151 and 152: P2X7 RECEPTOR ACTIVATION IS REQUIRE
Page 153 and 154: CASPASE-1 AND NLRP3 CONTRIBUTE TO T
Page 155: circulating Treg cells expressing C
Page 159 and 160: CHARACTERIZATION OF MONOCYTE SUBSET
Page 161 and 162: EFFECT OF OUABAIN IN MACROPHAGES IN
Page 163 and 164: OspC1 AND OspF: VIRULENCE FACTORS I
Page 165 and 166: Conclusion: Taken together, these r
Page 167 and 168: FIB produced higher proportion of f
Page 169 and 170: MØ phenotype M2, resulting in decr
Page 171 and 172: Financial support FAPESP, CAPES, CN
Page 173 and 174: ALTERATION IN THE DISTRIBUTION OF P
Page 175 and 176: BENEFICIAL EFFECTS OF PENTOXIFYLLIN
Page 177 and 178: MODULATION OF IMMUNE RESPONSE AND C
Page 179 and 180: FAS/FASL AND TRAIL CAUSE APOPTOSIS
Page 181 and 182: actin were more frequently detected
Page 183 and 184: NEUTROPHILS AND MACROPHAGES ARE INV
Page 185 and 186: EXPLORING THE INFLAMMATORY ROLE OF
Page 187 and 188: CERVICAL LEVELS OF INTERLEUKIN-1 BE
Page 189 and 190: Title: IDENTIFICATION BY PHAGE DISP
Page 191 and 192: PROFILE OF PRO- AND ANTI-INFLAMMATO
Page 193 and 194: IMMUNOMODULATORY MECHANISMS OF SOLU
Page 195 and 196: EVALUATION OF THE TH1, TH2 AND TH17
Page 197 and 198: production, as the blockade of PD-L
Page 199 and 200: CHARACTERIZATION OF POLYMORPHISM TN
Page 201 and 202: seems to be dependent on M. leprae
Page 203 and 204: 7 with tendency to normalization on
Page 205 and 206: CHARACTERIZATION OF THE CONSERVATIO
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ASC INFLAMMASOME IS NECESSARY TO AC
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CASPASE-1 IS REQUIRED TO CONTROL OF
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The role of CD4 + T lymphocytes dur
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CRITICAL ROLE OF MYD88 EXPRESSION I
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on ROS, cathepsin B and Syk kinase
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Financial Support: FAPESP and CNPq.
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80% in symptomatic group; 45% in as
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of cells undergoing apoptosis in DT
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Financial support: FAPESP/LIM-56/HC
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identify the active components and
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EVALUATION OF HUMORAL AND CELULLAR
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Financial support: CNPq-PIBIC, FAPE
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addition, significant expression of
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THE ROLE OF K + TRANSPORTERS IN THE
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Conclusion: In this study, we estab
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Heme activates oxidative mechanisms
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ROLE OF ADENOSINE AND PROSTAGLANDIN
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Taken together, our results indicat
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Conclusion: Our findings suggest th
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Financial support: CNPq, CAPES, IOC
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number of circulating parasites in
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CRITICAL ROLE OF IL-6 IN REGULATORY
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PPAR-α AGONIST GEMFIBROZIL DECREAS
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Cutaneous Leishmaniasis in Amazonas
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CYTOTOXICITY IN IMMUNOPATHOGENESIS
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ROS production and TLR2 and 4 expre
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ROLE OF IL-4 IN THE INTESTINAL INFL
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In vitro CULTURE SYSTEM FOR Plasmod
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interactions, Tandem Affinity Purif
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in the course of human infection wi
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findings suggest that IL-17 and IL-
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23 contribute to immunological cont
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not produced during infection by C.
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Conclusion: The production of cytok
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CONCLUSION: It is likely, in this p
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expression in CCC myocardium was 64
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that mononuclear cells derived from
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Conclusion: Altogether, our data sh
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PARACOCCIDIOIDES BRASILIENSIS INHIB
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CANINE VISCERAL LEISHMANIASIS AND S
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IMMUNOMODULATORY EFFECTS OF BIOTHER
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Leishmania EFFECT ON HEME CYTOTOXIC
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ROLE OF STRONGYLOIDES VENEZUELENSIS
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TREATMENT WITH Harpagophytum procum
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