immunology of infectious and parasitic diseases - XXXVII Congress ...

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LYMPHOID ORGANS BEHAVIOR AND DINAMIC OF LYMPHOCYTE POPULATIONS IN EXPERIMENTAL TRYPANOSOMA CRUZI ORAL INFECTION JULIANA BARRETO DE ALBUQUERQUE (PG)1; DÉSIO AURÉLIO FARIAS DE OLIVEIRA1; DANIELLE SILVA DOS SANTOS1; LUIZ RICARDO BERBERT1; CARLA EPONINA DE CARVALHO PINTO2; JOSÉ JURBERG1; VANDA CUNHA1; WILSON SAVINO1; JULIANA DE MEIS1 1Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Brazil; 2Immunobiology Institute, Federal Fluminense University, Brazil Introduction: Trypanosoma cruzi (T. cruzi), the causative agent of Chagas´ disease, infects about 10 million of people worldwide (WHO, 2010). The oral transmission by ingestion of contaminated food is causing outbreaks in several Brazilian states and other Latin America countries, and is potentially unrestricted to endemic regions (Int J Parasitol 39:615-623, 2009). Although we have previously described regional immune response in lymphoid organs in intraperitoneal (IP) murine infection (PLoS Negl Trop Dis 3(7):e417, 2009), there is a lack of data in oral infection/intragastric (IG). Here we analyze peripheral lymphoid organs behavior and dinamic of lymphocyte subpopulations in mice infected by IG or IP route. Methods and Results: BALB/c mice were infected with 5x104 T. cruzi trypomastigotes IP or IG and parasitemia was followed. After 9 (IP:n=8, IG:n=8), 12 (IP:n=3, IG:n=5), 15 (IP:n=1, IG:n=2), 17 (IP:n=1, IG:n=3) and 27 days (IG:n=3), subcutaneous (SCLN) and mesenteric lymph nodes (MLN), spleen (Sp) and Peyer patches (PP) were harvested for cell counting and flow cytometry. Hearts were harvested at 15 dpi (IP:n=3, IG:n=2) and sections stained with Hematoxilin and Eosin. IG infected mice presented lower parasitemia and mortality than IP infected mice. In IP infection, 95% of mice died before 18 dpi, while in IG group, 90% were still alive 27 dpi. SCLN (controls=19,5x106±2,9 cells; IP=57,6x106±9,1 cells; IG =31,8x106±3,2 cells) and Sp (controls=239,1x106±49,7 cells; IP=776,3x106±180,9 cells; IG=307,5x106±80,3 cells) showed increased number of cells in both groups 9 dpi and similar profile in later days; however, hypertrophy of these tissues as well as T and CD19+ cells expansion was less evident IG mice than IP. In mucosal-associated lymphoid tissues, such as PP, after 9 dpi the atrophy was more evident in IP mice than IG (controls=1,5x106±0,4 cells; IP=0,5 x 106±0,2 cells; IG=0,8x106±0,1 cells), due to decrease in CD19+ and CD4+ cells. MLN atrophy was only observed in after 15 dpi (controls= 40,1x106±4,5 cells; IP =11x 106 cells; IG=19,3x106±9,3 cells), due to depletion of CD19+ cells. Heart histological analysis showed similar alterations in 15 dpi (parasite nests, inflammatory infiltrate and tissue damage) in both groups, even with the lower
number of circulating parasites in IG mice. Conclusion: Our results indicate that the route of T. cruzi infection is a key factor to stimulate the immune response against the parasite in the host. Financial support: CNPq, FAPERJ, IOC/FIOCRUZ
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IMMUNOLOGY OF INFECTIOUS AND PARASI
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profiles appear to be dependent on
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INVOLVEMENT OF TNF RECEPTORS IN APO
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Effects of bioactive Cryptococcus n
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DCs expressing Indoleamine 2,3-diox
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DETECTION OF GITR ON PATIENT CERVIX
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DETECTION OF GITR AND IL-10 ON CERV
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VACCINATION WITH SM10.3 ANTIGEN, A
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Financial support: FAPESP, CAPES an
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clearance that follows Sylvio X10/4
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EhMSP-1 - AN ENTAMOEBA HISTOLYTICA
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THERAPY WITH SCFV TRANSFECTED-DENDR
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etween the groups. The results show
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Financial support: FIOCRUZ, CNPq.
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elease of NETs from human neutrophi
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ROLE OF CD18 IN ACTIVATION OF M1 MA
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IDENTIFICATION, PURIFICATION AND CH
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with hemin showed higher levels of
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SEPTIC ARTHRITIS TRIGGERED BY S. au
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eing higher levels induced by a vir
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parasite load skin. The TGF-β was
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cultures, both treatments decreased
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observed a tendency to lower parasi
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SERUM COMPONENTS AND MONONUCLEAR CE
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VIABILITY OF LEISHMANIA (L.) CHAGAS
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EVALUATION OF THE INTERFERENCE OF S
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IMMUNODETECTION OF THE TGF- β IN L
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DIFFERENT LEVELS OF NKT AND NK CELL
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Funding: CNPQ; FAPESPA; SESPA; UFPA
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saliva that exacerbated leishmania
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BACTERIAL FILAMENTATION: SUPER-RESI
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IMMUNOLOGICAL PARAMETERS ASSOCIATED
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supernatant. BMDC stimulation with
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such as number of eosinophils, prot
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EVALUATION OF THE PRODUCTION OF TNF
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Critical motifs of the Anaplasma ma
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SCHISTOSOMA SPP ANTIGENS IMPAIR THE
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DOWN-REGULATION OF IFN-g RECEPTOR A
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MONOCYTES SUBSETS IN SCHISTOSOMIASI
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THE IN VITRO ANALYSIS OF iNOS-KO MO
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CCR7 IS CRITICAL FOR RESISTANCE AGA
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EVALUATION OF THE IMMUNE RESPONSE A
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our data suggest that monocyte secr
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DIVERGENT OUTCOMES OF THE INTERACTI
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they can deactivate immune effector
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Conclusions: We observed a signific
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TLR2 AND TLR4 EXPRESSION AND NUTRIT
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DEVELOPMENT OF MURINE PLACENTAL MAL
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INDUCTION OF TH17 LYMPHOCYTES CONTR
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Leishmania amazonensis INCREASES EC
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TYPE II GRANULOMA INDUCED BY SCHIST
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The Role of Eosinophils in Aspergil
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compared to baseline (p
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disordered loop and NcSAG2A present
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Conclusion: We demonstrate the exis
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WT. By contrast, uninfected AhR -/-
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in the synthesis of proinflammatory
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PRODUCTION AND EVALUATION OF CHICKE
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allow a conclusion about the differ
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contributes to parasite control and
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Financial support: FAPESP, CNPq, an
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MILTEFOSINE EXERTS ITS LEISHMANICID
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IMMUNOMODULATORY ACTIVITIES OF Β-G
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REGULATION OF IMMUNE RESPONSE AGAIN
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THE ROLE OF REACTIVE OXIGEN SPECIES
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INNATE AND ADAPTIVE IMMUNE REPONSE
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KINETIC OF THE SPECIFIC HUMMORAL IM
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PURINERGIC RECEPTOR P2Y12 ACTIVATIO
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Evaluation of cytokine and chemokin
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Financial support: This research wa
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COMPARISON OF VIRULENCE AND IMMUNE
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THE IMPACT OF TUBERCULOSIS IN THE I
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GALACTOMANNAN ANTIGENEMIA SCREENING
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NATURAL ISOTYPIC RESPONSE AGAINST P
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Characterization of costimulatory m
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P2X7 RECEPTOR ACTIVATION IS REQUIRE
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CASPASE-1 AND NLRP3 CONTRIBUTE TO T
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circulating Treg cells expressing C
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PBMC, none of the treatments alter
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CHARACTERIZATION OF MONOCYTE SUBSET
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EFFECT OF OUABAIN IN MACROPHAGES IN
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OspC1 AND OspF: VIRULENCE FACTORS I
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Conclusion: Taken together, these r
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FIB produced higher proportion of f
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MØ phenotype M2, resulting in decr
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Financial support FAPESP, CAPES, CN
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ALTERATION IN THE DISTRIBUTION OF P
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BENEFICIAL EFFECTS OF PENTOXIFYLLIN
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MODULATION OF IMMUNE RESPONSE AND C
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FAS/FASL AND TRAIL CAUSE APOPTOSIS
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actin were more frequently detected
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NEUTROPHILS AND MACROPHAGES ARE INV
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EXPLORING THE INFLAMMATORY ROLE OF
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CERVICAL LEVELS OF INTERLEUKIN-1 BE
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Title: IDENTIFICATION BY PHAGE DISP
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PROFILE OF PRO- AND ANTI-INFLAMMATO
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IMMUNOMODULATORY MECHANISMS OF SOLU
Page 195 and 196: EVALUATION OF THE TH1, TH2 AND TH17
Page 197 and 198: production, as the blockade of PD-L
Page 199 and 200: CHARACTERIZATION OF POLYMORPHISM TN
Page 201 and 202: seems to be dependent on M. leprae
Page 203 and 204: 7 with tendency to normalization on
Page 205 and 206: CHARACTERIZATION OF THE CONSERVATIO
Page 207 and 208: ASC INFLAMMASOME IS NECESSARY TO AC
Page 209 and 210: CASPASE-1 IS REQUIRED TO CONTROL OF
Page 211 and 212: The role of CD4 + T lymphocytes dur
Page 213 and 214: CRITICAL ROLE OF MYD88 EXPRESSION I
Page 215 and 216: on ROS, cathepsin B and Syk kinase
Page 217 and 218: Financial Support: FAPESP and CNPq.
Page 219 and 220: 80% in symptomatic group; 45% in as
Page 221 and 222: of cells undergoing apoptosis in DT
Page 223 and 224: Financial support: FAPESP/LIM-56/HC
Page 225 and 226: identify the active components and
Page 227 and 228: EVALUATION OF HUMORAL AND CELULLAR
Page 229 and 230: Financial support: CNPq-PIBIC, FAPE
Page 231 and 232: addition, significant expression of
Page 233 and 234: THE ROLE OF K + TRANSPORTERS IN THE
Page 235 and 236: Conclusion: In this study, we estab
Page 237 and 238: Heme activates oxidative mechanisms
Page 239 and 240: ROLE OF ADENOSINE AND PROSTAGLANDIN
Page 241 and 242: Taken together, our results indicat
Page 243 and 244: Conclusion: Our findings suggest th
Page 245: Financial support: CNPq, CAPES, IOC
Page 249 and 250: CRITICAL ROLE OF IL-6 IN REGULATORY
Page 251 and 252: PPAR-α AGONIST GEMFIBROZIL DECREAS
Page 253 and 254: Cutaneous Leishmaniasis in Amazonas
Page 255 and 256: CYTOTOXICITY IN IMMUNOPATHOGENESIS
Page 257 and 258: ROS production and TLR2 and 4 expre
Page 259 and 260: ROLE OF IL-4 IN THE INTESTINAL INFL
Page 261 and 262: In vitro CULTURE SYSTEM FOR Plasmod
Page 263 and 264: interactions, Tandem Affinity Purif
Page 265 and 266: in the course of human infection wi
Page 267 and 268: findings suggest that IL-17 and IL-
Page 269 and 270: 23 contribute to immunological cont
Page 271 and 272: not produced during infection by C.
Page 273 and 274: Conclusion: The production of cytok
Page 275 and 276: CONCLUSION: It is likely, in this p
Page 277 and 278: expression in CCC myocardium was 64
Page 279 and 280: that mononuclear cells derived from
Page 281 and 282: Conclusion: Altogether, our data sh
Page 283 and 284: PARACOCCIDIOIDES BRASILIENSIS INHIB
Page 285 and 286: CANINE VISCERAL LEISHMANIASIS AND S
Page 287 and 288: IMMUNOMODULATORY EFFECTS OF BIOTHER
Page 289 and 290: Leishmania EFFECT ON HEME CYTOTOXIC
Page 291 and 292: ROLE OF STRONGYLOIDES VENEZUELENSIS
Page 293 and 294: TREATMENT WITH Harpagophytum procum
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