immunology of infectious and parasitic diseases - XXXVII Congress ...

HYPOINSULINEMIA-HYPERGLICEMIA CONDITION INTERFERES IN THE
OUTCOME OF INFLAMMATORY RESPONSE DURING FUNGAL SKIN
INFECTION IN A MURINE MODEL OF DIABETES MELLITU
BEATRIZ CESCHIM(IC)(1); LUIZA AYUMI NISHIYAMA MIMURA(IC)(1); JAMES
VENTURINI(PG)(1,2);MARIA SUELI PARREIRA DE ARRUDA(1).
(1)Faculdade de Ciências, UNESP - Univ Estadual Paulista, Bauru,
Departamento de Ciências Biológicas, Laboratório de Imunopatolgia
Experimental (LIPE);(2) Faculdade de Medicina, UNESP - Univ Estadual
Paulista, Botucatu, Programa de Pós-graduação em Doenças Tropicais.
Introdution: The high hyperglycemia observed in diabetic patients results in the
advanced glycation endproducts (AGEs) which are able to binding in receptors
present in the endothelial and immune cells that result in the induction of a variety
of cytokines. As a consequence, several disorders have been described in the
tissue matrix network such as the development of renal glomerulosclerosis and
the difficulties in the wound healing process in skin tissue. Considering that: 1)
hypoinsulinemia-hyperglicemia (HH) condition is associated with impaired
immune response to fungal infections, 2) infection in the diabetic foot is one of the
severe predisposing factors to amputation, 3) the mechanisms involved in skin
tissue wound healing during infection in diabetic patients are not fully understood;
in the present study, we evaluated the local inflammatory response and the
distribution of collagen and reticulin fibers in the footpad from HH-mice during
dermatophitic infection.
Matherial and Results: Male Swiss HH-mice, induced by alloxan (60mg/kg),
were submitted to subcutaneous inoculation of Trichophyton ntagrophytes into
the footpad. Non-infected HH-mice, free-HH infected-mice and non-infected and
free-HH mice were used as control groups. The mice were evaluated on days 7
and 14 after the fungal inoculation. The footpad were collected and submitted to
histological analyses by routine procedures for embedding in paraffinand. The
sections were stained with hematoxylin-eosin (HE), periodic acid-Schiff staining
(PAS), Masson trichrome and reticulin silver stain. Our results showed that the
introduction of the fungus in the footpad in free-HH mice resulted in an acute
inflammatory response followed by a granulomatous reaction; the deposition of
the collagen fibers and the thickness of reticular fibers were more intense on day