immunology of infectious and parasitic diseases - XXXVII Congress ...

Cutaneous Leishmaniasis in Amazonas, BR: Levels of citokines IL5 and
IL12 in patients infected by different species of Leishmania.
THAIS TIBERY ESPIR (1,2) ; LUANDA DE PAULA FIGUEIRA (1,2) ; MARICLEIDE
DE FARIAS NAIFF (2) ; ALLYSON GUIMARÃES DA COSTA (1,3) ; JOÃO PAULO
DINIZ PIMENTEL (3) ; ADRIANA MALHEIRO (1,3) ; ANTONIA MARIA RAMOS
FRANCO (2) .
(1). Federal University of Amazonas; (2) National Institute of Amazonia
Research; (3) Hematology and Hemotherapy Foundation of Amazonas.
Introduction: The northern region has the highest incidence of leishmaniasis in
Brazil, estimated at more than 2,000 new cases per year only in the state of
Amazonas. Clinical manifestations are related to different species of
Leishmania and the host immune response. The objective of this preliminary
study was to evaluate the immune response of patients infected with
Leishmania by analysis of cytokines and different species involved in the
disease. Methods and Results: This study of case series was conducted from
June 2009 to February 2012, from five cities in the state of Amazonas. Was
analyzed twelve patients aged between 21 and 58 years old, with the duration
of the injury about a month in 83% of cases, most activities in the forest area in
100% of cases. Species were characterized as L. amazonensis, L. braziliensis
and L. guyanensis by isoenzyme electrophoresis technique. Cytokines were
measured in the sera by ELISA (BD Biosciences). The levels of IL-12 in the
sera of patients infected with all species, was 68% higher after treatment with
pentavalent antimony N-methylglucamine compared with samples before the
treatment, the level of IL-5 showed inverse proportion compared to IL-12 and
the level of IL-5 had higher elevation before treatment with the same drug. The
predominant parasite species was L. guyanensis (80%) and there was no
significant difference (p> 0.005) in cytokine levels before and after treatment to
infection by different species. Conclusion: The data presented contributes to
the understanding of the immune response to infection by species of
Leishmania. It is noted that the determination of Th1 and Th2 responses can be
influenced differently depending on the species of Leishmania and other factors
in addition to the genetic and immunological features of the host.
FINANCIAL SUPPORT: PPSUS/FAPEAM, CAPES.