immunology of infectious and parasitic diseases - XXXVII Congress ...

ALTERATION IN THE DISTRIBUTION OF PERITONEAL MACROPHAGE
SUBSETS IN A MURINE MODEL OF DIABETES MELLITUS DURING
FUNGAL INFECTION.
THAIS FERNANDA DE CAMPOS FRAGA-SILVA(PG)(1,4); CAMILA MARTINS
MARCHETTI(PG)(2,4); MARJORIE DE ASSIS GOLIM(PG)(3); JAMES
VENTURINI(PG)(2,4); MARIA SUELI PARREIRA DE ARRUDA(4).
(1) Instituto de Biociências, UNESP - Univ Estadual Paulista, Botucatu,
Programa de Pós-Graduação em Biologia Geral e Aplicada; (2) Faculdade de
Medicina de Botucatu, UNESP - Univ Estadual Paulista, Botucatu, Programa de
Pós-graduação em Doenças Tropicais; (3) Faculdade de Medicina de Botucatu,
UNESP - Univ Estadual Paulista, Botucatu, Laboratório de Citometria de Fluxo
do Hemocentro de Botucatu; (4) Faculdade de Ciências, UNESP - Univ
Estadual Paulista, Bauru, Departamento de Ciências Biológicas, Laboratório de
Imunopatologia Experimental (LIPE).
Introduction: Macrophages (MØ) display heterogeneous phenotype according
to the distribution to different tissue and the cytokine-chemokine networks.
Recently, two peritoneal MØ subsets were described under homeostatic
condition according to morphological size and CD11b and F4/80 expression.
The small peritoneal MØ (SPMs) is identified for exhibit lower expression of
CD11b and F4/80 and the large peritoneal MØ (LPMs) for exhibit high levels of
the CD11b and F4/80. Considering that the distribution of this cells has
not been studied in pathological condition such as fungal infection neither in
Diabetes Mellitus, in the present study we investigated the distribution of SPM
and LPM in the peritoneal cavity from hypoinsulinemic-hyperglicemic (HH)-mice
during dermatophytic infection. Methods and Results: HH-mice, induced by
alloxan (60mg/kg), were inoculated into the footpad with 10 7 Trichophyton
mentagrophytes conidia (Group HHTM). Non-infected HH-mice (HH), only
infected-mice (TM), non-infected and free-HH mice (CTRL) were used as
control groups. The mice were evaluated at 24 and 48 hours and 7 days after
the inoculation. Fragments of the footpad, popliteal lymph node, liver, spleen
and kidney were submitted to determination of the fungal load. The distribution
of peritoneal MØ subsets was performed by flow cytometry. Our results showed
that mice from TM group exhibited an increase of LPMs soon after the
fungal introduction. Overtime, they exhibited a reduction of fungal load and, the
percentage of this subset returned to normal levels. In the HH group, we
observed an increase of the LPMs at all times evaluated. In the HHTM group
the distribution of LPMs was similar to the TM group, except on day 7, which the
mice exhibited a lower percentage of LPMs in comparasion to HH-mice. The
percentage of SPMs in HHTM group was always lower than in the HH group; in
the TM group, this phenomenon was only observed on day 7. Conclusion: Our
results demonstrated that both infectious and HH conditions triggered changes
in the distribution of SPM and LPM. Thus, factors from the fungus such as