immunology of infectious and parasitic diseases - XXXVII Congress ...

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EFFECT OF AUTHENTIC PGE2 PRODUCED BY Paracoccidioides brasiliensis ON EFFECTOR FUNCTIONS OF HUMAN POLYMORPHONUCLEAR AND MONONUCLEAR CELLS ANA PAULA BORDON-GRACIANI (1), GUILHERME AUGUSTO BIONDO (PG) (1), LUCIANE ALARCÃO DIAS-MELICIO (2), TATIANA FERNANDA BACHIEGA (PG) (1), MARJORIE DE ASSIS GOLIM (3), JOSÉ ROBERTO MARQUES SILVA (4), ÂNGELA MARIA VICTORIANO DE CAMPOS SOARES (1). (1) Departamento de Microbiologia e Imunologia, Instituto de Biociências de Botucatu, UNESP – Univ Estadual Paulista, Brasil; (2) Departamento de Patologia, Faculdade de Medicina de Botucatu, UNESP – Univ Estadual Paulista, Brasil; (3) Hemocentro, Faculdade de Medicina de Botucatu, UNESP – Univ Estadual Paulista, Brasil; (4) Núcleo de Pesquisa Avançada em Matologia, Faculdade de Ciências Agronômicas de Botucatu, UNESP – Univ Estadual Paulista, Brasil. Introduction: Prostaglandins (PGs) are eicosanoids synthesized from arachidonic acid oxidation that regulate key aspects of the immune response in different infections. In paracoccidioidomycosis, human monocytes produce PGs that inhibit the fungicidal activity of these cells in vitro, by reducing H2O2 and TNF- production. In addition to mammalian cells, another potential source of PGE2 is the fungal pathogen itself. We demonstrated that P. brasiliensis is able to produce authentic PGE2, which is directly involved in the survival of this fungus. However, the function of PGE2 produced by this fungus on host cell activities has not yet been evaluated. Thus, the purpose of this work was to assess the effect of PGE2 produced by P. brasiliensis on the effectors functions of human polymorphonuclear and mononuclear cells. Methods and Results: Peripheral blood neutrophils (PMN), monocytes (MO) and PBMC obtained from 10 healthy donors were treated with PGE2 purified from P. brasiliensis yeast cells cultures (PGE2f) or commercial PGE2 (PGE2c). After 1, 2, 4, 8 and 12 hours of incubation, PMN and MO cultures were evaluated by phagocytosis assay, surface receptors expression (TLR-2, TLR4, dectin-1, MR and HLA-DR), H2O2, cytokines (TNF-α, IL-6, IL-8, IL-10, IL-12p70, IL-15, IL-17, IL-23, IL-27) and chemokines (MCP-1 and MIP-1α) production. PBMC cultures were evaluated after 24, 48 and 72 hours of incubation with PGE2f and PGE2c by cellular proliferation and cytokines production (TNF-α, IFN-, IL-2, IL-4, IL-10, IL-12 and IL-17). The treatment with PGE2c or PGE2f inhibited the phagocytosis of zymozan and H2O2 production by PMN and MO cultures stimulated with PMA. The treatment also inhibited TLR-2 on PMN surface and HLA-DR expression on MO, while MR expression was increased. There were a decrease on TNF-α and MCP-1 production, and an increase on IL-6 production by PMNs. MO showed a decrease on TNF-α, IL-12, IL-15 and MCP-1 levels and an increase on IL-6, IL-10, IL-17 and IL-23 production. In relation to PBMC
cultures, both treatments decreased cellular proliferation and TNF-α, IFN-, IL-2 and IL-12 levels, but increased IL-4, IL-10 and IL-17 production. Conclusion: Our results show that PGE2 purified from P. brasiliensis cultures has the same immunomodulatory effects on mononuclear and polymorphonuclear human cells when compared with PGE2c. Financial support: FAPESP 2009/13751-9 and FAPESP 2009/08938-2.
Page 1 and 2: IMMUNOLOGY OF INFECTIOUS AND PARASI
Page 3 and 4: profiles appear to be dependent on
Page 5 and 6: INVOLVEMENT OF TNF RECEPTORS IN APO
Page 7 and 8: Effects of bioactive Cryptococcus n
Page 9 and 10: DCs expressing Indoleamine 2,3-diox
Page 11 and 12: DETECTION OF GITR ON PATIENT CERVIX
Page 13 and 14: DETECTION OF GITR AND IL-10 ON CERV
Page 15 and 16: VACCINATION WITH SM10.3 ANTIGEN, A
Page 17 and 18: Financial support: FAPESP, CAPES an
Page 19 and 20: clearance that follows Sylvio X10/4
Page 21 and 22: EhMSP-1 - AN ENTAMOEBA HISTOLYTICA
Page 23 and 24: THERAPY WITH SCFV TRANSFECTED-DENDR
Page 25 and 26: etween the groups. The results show
Page 27 and 28: Financial support: FIOCRUZ, CNPq.
Page 29 and 30: elease of NETs from human neutrophi
Page 31 and 32: ROLE OF CD18 IN ACTIVATION OF M1 MA
Page 33 and 34: IDENTIFICATION, PURIFICATION AND CH
Page 35 and 36: with hemin showed higher levels of
Page 37 and 38: SEPTIC ARTHRITIS TRIGGERED BY S. au
Page 39 and 40: eing higher levels induced by a vir
Page 41: parasite load skin. The TGF-β was
Page 45 and 46: observed a tendency to lower parasi
Page 47 and 48: SERUM COMPONENTS AND MONONUCLEAR CE
Page 49 and 50: VIABILITY OF LEISHMANIA (L.) CHAGAS
Page 51 and 52: EVALUATION OF THE INTERFERENCE OF S
Page 53 and 54: IMMUNODETECTION OF THE TGF- β IN L
Page 55 and 56: DIFFERENT LEVELS OF NKT AND NK CELL
Page 57 and 58: Funding: CNPQ; FAPESPA; SESPA; UFPA
Page 59 and 60: saliva that exacerbated leishmania
Page 61 and 62: BACTERIAL FILAMENTATION: SUPER-RESI
Page 63 and 64: IMMUNOLOGICAL PARAMETERS ASSOCIATED
Page 65 and 66: supernatant. BMDC stimulation with
Page 67 and 68: such as number of eosinophils, prot
Page 69 and 70: EVALUATION OF THE PRODUCTION OF TNF
Page 71 and 72: Critical motifs of the Anaplasma ma
Page 73 and 74: SCHISTOSOMA SPP ANTIGENS IMPAIR THE
Page 75 and 76: DOWN-REGULATION OF IFN-g RECEPTOR A
Page 77 and 78: MONOCYTES SUBSETS IN SCHISTOSOMIASI
Page 79 and 80: THE IN VITRO ANALYSIS OF iNOS-KO MO
Page 81 and 82: CCR7 IS CRITICAL FOR RESISTANCE AGA
Page 83 and 84: EVALUATION OF THE IMMUNE RESPONSE A
Page 85 and 86: our data suggest that monocyte secr
Page 87 and 88: DIVERGENT OUTCOMES OF THE INTERACTI
Page 89 and 90: they can deactivate immune effector
Page 91 and 92: Conclusions: We observed a signific
Page 93 and 94:
TLR2 AND TLR4 EXPRESSION AND NUTRIT
Page 95 and 96:
DEVELOPMENT OF MURINE PLACENTAL MAL
Page 97 and 98:
INDUCTION OF TH17 LYMPHOCYTES CONTR
Page 99 and 100:
Leishmania amazonensis INCREASES EC
Page 101 and 102:
TYPE II GRANULOMA INDUCED BY SCHIST
Page 103 and 104:
The Role of Eosinophils in Aspergil
Page 105 and 106:
compared to baseline (p
Page 107 and 108:
disordered loop and NcSAG2A present
Page 109 and 110:
Conclusion: We demonstrate the exis
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WT. By contrast, uninfected AhR -/-
Page 113 and 114:
in the synthesis of proinflammatory
Page 115 and 116:
PRODUCTION AND EVALUATION OF CHICKE
Page 117 and 118:
allow a conclusion about the differ
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contributes to parasite control and
Page 121 and 122:
Financial support: FAPESP, CNPq, an
Page 123 and 124:
MILTEFOSINE EXERTS ITS LEISHMANICID
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IMMUNOMODULATORY ACTIVITIES OF Β-G
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REGULATION OF IMMUNE RESPONSE AGAIN
Page 129 and 130:
THE ROLE OF REACTIVE OXIGEN SPECIES
Page 131 and 132:
INNATE AND ADAPTIVE IMMUNE REPONSE
Page 133 and 134:
KINETIC OF THE SPECIFIC HUMMORAL IM
Page 135 and 136:
PURINERGIC RECEPTOR P2Y12 ACTIVATIO
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Evaluation of cytokine and chemokin
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Financial support: This research wa
Page 141 and 142:
COMPARISON OF VIRULENCE AND IMMUNE
Page 143 and 144:
THE IMPACT OF TUBERCULOSIS IN THE I
Page 145 and 146:
GALACTOMANNAN ANTIGENEMIA SCREENING
Page 147 and 148:
NATURAL ISOTYPIC RESPONSE AGAINST P
Page 149 and 150:
Characterization of costimulatory m
Page 151 and 152:
P2X7 RECEPTOR ACTIVATION IS REQUIRE
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CASPASE-1 AND NLRP3 CONTRIBUTE TO T
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circulating Treg cells expressing C
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PBMC, none of the treatments alter
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CHARACTERIZATION OF MONOCYTE SUBSET
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EFFECT OF OUABAIN IN MACROPHAGES IN
Page 163 and 164:
OspC1 AND OspF: VIRULENCE FACTORS I
Page 165 and 166:
Conclusion: Taken together, these r
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FIB produced higher proportion of f
Page 169 and 170:
MØ phenotype M2, resulting in decr
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Financial support FAPESP, CAPES, CN
Page 173 and 174:
ALTERATION IN THE DISTRIBUTION OF P
Page 175 and 176:
BENEFICIAL EFFECTS OF PENTOXIFYLLIN
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MODULATION OF IMMUNE RESPONSE AND C
Page 179 and 180:
FAS/FASL AND TRAIL CAUSE APOPTOSIS
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actin were more frequently detected
Page 183 and 184:
NEUTROPHILS AND MACROPHAGES ARE INV
Page 185 and 186:
EXPLORING THE INFLAMMATORY ROLE OF
Page 187 and 188:
CERVICAL LEVELS OF INTERLEUKIN-1 BE
Page 189 and 190:
Title: IDENTIFICATION BY PHAGE DISP
Page 191 and 192:
PROFILE OF PRO- AND ANTI-INFLAMMATO
Page 193 and 194:
IMMUNOMODULATORY MECHANISMS OF SOLU
Page 195 and 196:
EVALUATION OF THE TH1, TH2 AND TH17
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production, as the blockade of PD-L
Page 199 and 200:
CHARACTERIZATION OF POLYMORPHISM TN
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seems to be dependent on M. leprae
Page 203 and 204:
7 with tendency to normalization on
Page 205 and 206:
CHARACTERIZATION OF THE CONSERVATIO
Page 207 and 208:
ASC INFLAMMASOME IS NECESSARY TO AC
Page 209 and 210:
CASPASE-1 IS REQUIRED TO CONTROL OF
Page 211 and 212:
The role of CD4 + T lymphocytes dur
Page 213 and 214:
CRITICAL ROLE OF MYD88 EXPRESSION I
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on ROS, cathepsin B and Syk kinase
Page 217 and 218:
Financial Support: FAPESP and CNPq.
Page 219 and 220:
80% in symptomatic group; 45% in as
Page 221 and 222:
of cells undergoing apoptosis in DT
Page 223 and 224:
Financial support: FAPESP/LIM-56/HC
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identify the active components and
Page 227 and 228:
EVALUATION OF HUMORAL AND CELULLAR
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Financial support: CNPq-PIBIC, FAPE
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addition, significant expression of
Page 233 and 234:
THE ROLE OF K + TRANSPORTERS IN THE
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Conclusion: In this study, we estab
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Heme activates oxidative mechanisms
Page 239 and 240:
ROLE OF ADENOSINE AND PROSTAGLANDIN
Page 241 and 242:
Taken together, our results indicat
Page 243 and 244:
Conclusion: Our findings suggest th
Page 245 and 246:
Financial support: CNPq, CAPES, IOC
Page 247 and 248:
number of circulating parasites in
Page 249 and 250:
CRITICAL ROLE OF IL-6 IN REGULATORY
Page 251 and 252:
PPAR-α AGONIST GEMFIBROZIL DECREAS
Page 253 and 254:
Cutaneous Leishmaniasis in Amazonas
Page 255 and 256:
CYTOTOXICITY IN IMMUNOPATHOGENESIS
Page 257 and 258:
ROS production and TLR2 and 4 expre
Page 259 and 260:
ROLE OF IL-4 IN THE INTESTINAL INFL
Page 261 and 262:
In vitro CULTURE SYSTEM FOR Plasmod
Page 263 and 264:
interactions, Tandem Affinity Purif
Page 265 and 266:
in the course of human infection wi
Page 267 and 268:
findings suggest that IL-17 and IL-
Page 269 and 270:
23 contribute to immunological cont
Page 271 and 272:
not produced during infection by C.
Page 273 and 274:
Conclusion: The production of cytok
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CONCLUSION: It is likely, in this p
Page 277 and 278:
expression in CCC myocardium was 64
Page 279 and 280:
that mononuclear cells derived from
Page 281 and 282:
Conclusion: Altogether, our data sh
Page 283 and 284:
PARACOCCIDIOIDES BRASILIENSIS INHIB
Page 285 and 286:
CANINE VISCERAL LEISHMANIASIS AND S
Page 287 and 288:
IMMUNOMODULATORY EFFECTS OF BIOTHER
Page 289 and 290:
Leishmania EFFECT ON HEME CYTOTOXIC
Page 291 and 292:
ROLE OF STRONGYLOIDES VENEZUELENSIS
Page 293 and 294:
TREATMENT WITH Harpagophytum procum
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