immunology of infectious and parasitic diseases - XXXVII Congress ...

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PATTERN RECOGNIZING RECEPTORS (PRRs) INVOLVED IN EICOSANOID PRODUCTION BY HUMAN NEUTROPHILS IN RESPONSE TO Paracoccidioides brasiliensis HELANDERSON DE ALMEIDA BALDERRAMAS (PG) 1 ; DANIELA RAMOS RODRIGUES (PG) 1 ; TATIANA FERNANDA BACHIEGA (PG) 1 ; REGINALDO KELLER FERNANDES (PG) 1 ; LUCIANE ALARCÃO DIAS-MELICIO 2 ; MARCIMARA PENITENTI 3 ; MAURA ROSANE VALÉRIO IKOMA 3 ; SILVIO LUIS DE OLIVEIRA 1 ; ÂNGELA MARIA VICTORIANO DE CAMPOS SOARES 1 1 Microbiology and Immunology Department, Bioscience Institute, UNESP – Univ. Estadual Paulista, Botucatu-SP, Brazil; 2 Department of Pathology, Botucatu Medical School, UNESP – Univ. Estadual Paulista, Brazil; 3 Amaral Carvalho Foundation - Flow cytometry Laboratory, Jaú-SP, Brazil. Introduction and Objective: Paracoccidioides brasiliensis is the agent of paracoccidioidomycosis, the most prevalent deep mycosis in Latin America. Since the etiological agent is considered an intracellular pathogen, studies of its interaction with phagocytes are essential for the understanding of the hostparasite relationship. The interaction between PAMPS (pathogen-associated molecular patterns) and PRRs leads to the activation of phagocytic cells with subsequent production of mediators which modulate antimicrobial activities of these cells. Among these mediators the eicosanoids PGE2 and LTB4 have received attention in recent years. Studies on our laboratory have shown that human monocytes in response to P.brasiliensis produce PGE2 and LTB4 that respectively inhibits and increases antifungal activities of these cells. In this context, we aimed to evaluate whether human neutrophils produce LTB4 and PGE2 in response to high (Pb18) and low virulent strain (Pb265) of P.brasiliensis and which PRRs are involved. Methods and Results: Neutrophils were treated with monoclonal antibodies anti-TLR2, anti-mannose or anti-dectin-1 for 2h followed by challenge with Pb18 or Pb265 for 4 and 18h. After these periods, supernatants were removed and evaluated for LTB4 and PGE2 levels, by Elisa. We detected that control cells after 4h produce low amounts of PGE2 that was significantly increased after challenge with the fungus, mainly with Pb18. At 18h, PGE2 levels induced by both strains were proportionally increased in comparison to previous period. Blocking assays at 4h revealed that dectin-1 and mannose receptor (MR) were involved in this production. However, at 18h, the role of these receptors was not so clear. In relation to LTB4, the results showed that Pb18 and Pb265 induce similar levels of this eicosanoid. In addition, this production was similar after 4 and 18h. Of note, differently from PGE2 results, blocking assays revealed that none of the tested receptors was involved in LTB4 production. Conclusions: P.brasiliensis by binding to dectin-1 and MR induces PGE2 production by human neutrophils,
eing higher levels induced by a virulent strain. This difference could represent high capacity of this strain to escape from antifungal mechanisms of neutrophils, since PGE2 could inhibit some functions of these cells. This fungus also induces LTB4 production by neutrophils, however by a mechanism independent from dectin-1, TLR2 or MR.
Page 1 and 2: IMMUNOLOGY OF INFECTIOUS AND PARASI
Page 3 and 4: profiles appear to be dependent on
Page 5 and 6: INVOLVEMENT OF TNF RECEPTORS IN APO
Page 7 and 8: Effects of bioactive Cryptococcus n
Page 9 and 10: DCs expressing Indoleamine 2,3-diox
Page 11 and 12: DETECTION OF GITR ON PATIENT CERVIX
Page 13 and 14: DETECTION OF GITR AND IL-10 ON CERV
Page 15 and 16: VACCINATION WITH SM10.3 ANTIGEN, A
Page 17 and 18: Financial support: FAPESP, CAPES an
Page 19 and 20: clearance that follows Sylvio X10/4
Page 21 and 22: EhMSP-1 - AN ENTAMOEBA HISTOLYTICA
Page 23 and 24: THERAPY WITH SCFV TRANSFECTED-DENDR
Page 25 and 26: etween the groups. The results show
Page 27 and 28: Financial support: FIOCRUZ, CNPq.
Page 29 and 30: elease of NETs from human neutrophi
Page 31 and 32: ROLE OF CD18 IN ACTIVATION OF M1 MA
Page 33 and 34: IDENTIFICATION, PURIFICATION AND CH
Page 35 and 36: with hemin showed higher levels of
Page 37: SEPTIC ARTHRITIS TRIGGERED BY S. au
Page 41 and 42: parasite load skin. The TGF-β was
Page 43 and 44: cultures, both treatments decreased
Page 45 and 46: observed a tendency to lower parasi
Page 47 and 48: SERUM COMPONENTS AND MONONUCLEAR CE
Page 49 and 50: VIABILITY OF LEISHMANIA (L.) CHAGAS
Page 51 and 52: EVALUATION OF THE INTERFERENCE OF S
Page 53 and 54: IMMUNODETECTION OF THE TGF- β IN L
Page 55 and 56: DIFFERENT LEVELS OF NKT AND NK CELL
Page 57 and 58: Funding: CNPQ; FAPESPA; SESPA; UFPA
Page 59 and 60: saliva that exacerbated leishmania
Page 61 and 62: BACTERIAL FILAMENTATION: SUPER-RESI
Page 63 and 64: IMMUNOLOGICAL PARAMETERS ASSOCIATED
Page 65 and 66: supernatant. BMDC stimulation with
Page 67 and 68: such as number of eosinophils, prot
Page 69 and 70: EVALUATION OF THE PRODUCTION OF TNF
Page 71 and 72: Critical motifs of the Anaplasma ma
Page 73 and 74: SCHISTOSOMA SPP ANTIGENS IMPAIR THE
Page 75 and 76: DOWN-REGULATION OF IFN-g RECEPTOR A
Page 77 and 78: MONOCYTES SUBSETS IN SCHISTOSOMIASI
Page 79 and 80: THE IN VITRO ANALYSIS OF iNOS-KO MO
Page 81 and 82: CCR7 IS CRITICAL FOR RESISTANCE AGA
Page 83 and 84: EVALUATION OF THE IMMUNE RESPONSE A
Page 85 and 86: our data suggest that monocyte secr
Page 87 and 88: DIVERGENT OUTCOMES OF THE INTERACTI
Page 89 and 90:
they can deactivate immune effector
Page 91 and 92:
Conclusions: We observed a signific
Page 93 and 94:
TLR2 AND TLR4 EXPRESSION AND NUTRIT
Page 95 and 96:
DEVELOPMENT OF MURINE PLACENTAL MAL
Page 97 and 98:
INDUCTION OF TH17 LYMPHOCYTES CONTR
Page 99 and 100:
Leishmania amazonensis INCREASES EC
Page 101 and 102:
TYPE II GRANULOMA INDUCED BY SCHIST
Page 103 and 104:
The Role of Eosinophils in Aspergil
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compared to baseline (p
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disordered loop and NcSAG2A present
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Conclusion: We demonstrate the exis
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WT. By contrast, uninfected AhR -/-
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in the synthesis of proinflammatory
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PRODUCTION AND EVALUATION OF CHICKE
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allow a conclusion about the differ
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contributes to parasite control and
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Financial support: FAPESP, CNPq, an
Page 123 and 124:
MILTEFOSINE EXERTS ITS LEISHMANICID
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IMMUNOMODULATORY ACTIVITIES OF Β-G
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REGULATION OF IMMUNE RESPONSE AGAIN
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THE ROLE OF REACTIVE OXIGEN SPECIES
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INNATE AND ADAPTIVE IMMUNE REPONSE
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KINETIC OF THE SPECIFIC HUMMORAL IM
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PURINERGIC RECEPTOR P2Y12 ACTIVATIO
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Evaluation of cytokine and chemokin
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Financial support: This research wa
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COMPARISON OF VIRULENCE AND IMMUNE
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THE IMPACT OF TUBERCULOSIS IN THE I
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GALACTOMANNAN ANTIGENEMIA SCREENING
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NATURAL ISOTYPIC RESPONSE AGAINST P
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Characterization of costimulatory m
Page 151 and 152:
P2X7 RECEPTOR ACTIVATION IS REQUIRE
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CASPASE-1 AND NLRP3 CONTRIBUTE TO T
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circulating Treg cells expressing C
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PBMC, none of the treatments alter
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CHARACTERIZATION OF MONOCYTE SUBSET
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EFFECT OF OUABAIN IN MACROPHAGES IN
Page 163 and 164:
OspC1 AND OspF: VIRULENCE FACTORS I
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Conclusion: Taken together, these r
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FIB produced higher proportion of f
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MØ phenotype M2, resulting in decr
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Financial support FAPESP, CAPES, CN
Page 173 and 174:
ALTERATION IN THE DISTRIBUTION OF P
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BENEFICIAL EFFECTS OF PENTOXIFYLLIN
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MODULATION OF IMMUNE RESPONSE AND C
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FAS/FASL AND TRAIL CAUSE APOPTOSIS
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actin were more frequently detected
Page 183 and 184:
NEUTROPHILS AND MACROPHAGES ARE INV
Page 185 and 186:
EXPLORING THE INFLAMMATORY ROLE OF
Page 187 and 188:
CERVICAL LEVELS OF INTERLEUKIN-1 BE
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Title: IDENTIFICATION BY PHAGE DISP
Page 191 and 192:
PROFILE OF PRO- AND ANTI-INFLAMMATO
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IMMUNOMODULATORY MECHANISMS OF SOLU
Page 195 and 196:
EVALUATION OF THE TH1, TH2 AND TH17
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production, as the blockade of PD-L
Page 199 and 200:
CHARACTERIZATION OF POLYMORPHISM TN
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seems to be dependent on M. leprae
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7 with tendency to normalization on
Page 205 and 206:
CHARACTERIZATION OF THE CONSERVATIO
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ASC INFLAMMASOME IS NECESSARY TO AC
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CASPASE-1 IS REQUIRED TO CONTROL OF
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The role of CD4 + T lymphocytes dur
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CRITICAL ROLE OF MYD88 EXPRESSION I
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on ROS, cathepsin B and Syk kinase
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Financial Support: FAPESP and CNPq.
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80% in symptomatic group; 45% in as
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of cells undergoing apoptosis in DT
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Financial support: FAPESP/LIM-56/HC
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identify the active components and
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EVALUATION OF HUMORAL AND CELULLAR
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Financial support: CNPq-PIBIC, FAPE
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addition, significant expression of
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THE ROLE OF K + TRANSPORTERS IN THE
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Conclusion: In this study, we estab
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Heme activates oxidative mechanisms
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ROLE OF ADENOSINE AND PROSTAGLANDIN
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Taken together, our results indicat
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Conclusion: Our findings suggest th
Page 245 and 246:
Financial support: CNPq, CAPES, IOC
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number of circulating parasites in
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CRITICAL ROLE OF IL-6 IN REGULATORY
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PPAR-α AGONIST GEMFIBROZIL DECREAS
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Cutaneous Leishmaniasis in Amazonas
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CYTOTOXICITY IN IMMUNOPATHOGENESIS
Page 257 and 258:
ROS production and TLR2 and 4 expre
Page 259 and 260:
ROLE OF IL-4 IN THE INTESTINAL INFL
Page 261 and 262:
In vitro CULTURE SYSTEM FOR Plasmod
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interactions, Tandem Affinity Purif
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in the course of human infection wi
Page 267 and 268:
findings suggest that IL-17 and IL-
Page 269 and 270:
23 contribute to immunological cont
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not produced during infection by C.
Page 273 and 274:
Conclusion: The production of cytok
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CONCLUSION: It is likely, in this p
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expression in CCC myocardium was 64
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that mononuclear cells derived from
Page 281 and 282:
Conclusion: Altogether, our data sh
Page 283 and 284:
PARACOCCIDIOIDES BRASILIENSIS INHIB
Page 285 and 286:
CANINE VISCERAL LEISHMANIASIS AND S
Page 287 and 288:
IMMUNOMODULATORY EFFECTS OF BIOTHER
Page 289 and 290:
Leishmania EFFECT ON HEME CYTOTOXIC
Page 291 and 292:
ROLE OF STRONGYLOIDES VENEZUELENSIS
Page 293 and 294:
TREATMENT WITH Harpagophytum procum
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