immunology of infectious and parasitic diseases - XXXVII Congress ...

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13173 - COMPARISON OF ANTI-LEISHMANIAL IGG ANTIBODIES PROFILE IN SERA FROM BRAZILIAN PATIENTS WITH VISCERAL LEISHMANIASIS AND INDIVIDUALS PRESENTING ASYMPTOMATIC INFECTIONS LUIZ GUSTAVO ARAUJO GARDINASSI 1 ; GUSTAVO ROCHA GARCIA 1 ; SANDRA REGINA MARUYAMA 1 ; FERNANDO OLIVEIRA DA SILVA 2 ; CARLOS HENRIQUE NERY COSTA 2 ; ROQUE PACHECO DE ALMEIDA 3 ; AMÉLIA MARIA RIBEIRO DE JESUS 3 ; ISABEL KINNEY FERREIRA DE MIRANDA SANTOS 1 1 Faculdade de Medicina de Ribeirão Preto - USP, Departamento de Bioquímica e Imunologia; 2 Instituto de Doenças Tropicais Natan Portella - UFPI; 3 Centro de Ciências Biológicas e da Saúde – UFS, Depto. de Medicina Interna e Patologia Introduction: It is intriguing that the majority of infections with the etiological agent of visceral leishmaniasis (VL), Leishmania infantum, do not result in disease. The scientific consensus is that antibodies do not have a role in this outcome and, in fact, participate in susceptibility to VL; however recent studies challenge this view. We aim to test the hypothesis that individuals presenting with asymptomatic infections with L. infantum remain so because they produce antibodies with functional properties that differ from those produced by individuals who develop VL. Methods and Results: With immunoblots and ELISAs we will study the correlation of different outcomes of infections with L. infantum (VL or asymptomatic infection) with antigen reactivity profiles and titers of IgG1-4 antibodies in serum from humans of different ages. Description of patterns of IgG antibody responses will be refined by probing sera with a protein microarray constructed with approximately 2300 secreted or plasma membrane proteins of L. infantum. Sera were collected from 208 kala-azar patients, 197 from individuals with asymptomatic infection and 138 from healthy donors, all living in endemic areas. Western blots were performed between soluble antigens (SLA) of L. infantum and sera from individuals presenting with VL or infected asymptomatically. Indeed, sera from VL patients recognize a set of antigens that is distinct from those recognized by sera from infected, asymptomatic individuals, and the pattern of recognition differs both by specificity and intensity. ELISAs were performed to screen IgG antibodies against SLA
etween the groups. The results show that VL patients present higher titers (~10 fold) of IgG against SLA than asymptomatic carriers. Despite this data, a statistically significant difference between asymptomatic individuals and healthy controls was obtained (P
Page 1 and 2: IMMUNOLOGY OF INFECTIOUS AND PARASI
Page 3 and 4: profiles appear to be dependent on
Page 5 and 6: INVOLVEMENT OF TNF RECEPTORS IN APO
Page 7 and 8: Effects of bioactive Cryptococcus n
Page 9 and 10: DCs expressing Indoleamine 2,3-diox
Page 11 and 12: DETECTION OF GITR ON PATIENT CERVIX
Page 13 and 14: DETECTION OF GITR AND IL-10 ON CERV
Page 15 and 16: VACCINATION WITH SM10.3 ANTIGEN, A
Page 17 and 18: Financial support: FAPESP, CAPES an
Page 19 and 20: clearance that follows Sylvio X10/4
Page 21 and 22: EhMSP-1 - AN ENTAMOEBA HISTOLYTICA
Page 23: THERAPY WITH SCFV TRANSFECTED-DENDR
Page 27 and 28: Financial support: FIOCRUZ, CNPq.
Page 29 and 30: elease of NETs from human neutrophi
Page 31 and 32: ROLE OF CD18 IN ACTIVATION OF M1 MA
Page 33 and 34: IDENTIFICATION, PURIFICATION AND CH
Page 35 and 36: with hemin showed higher levels of
Page 37 and 38: SEPTIC ARTHRITIS TRIGGERED BY S. au
Page 39 and 40: eing higher levels induced by a vir
Page 41 and 42: parasite load skin. The TGF-β was
Page 43 and 44: cultures, both treatments decreased
Page 45 and 46: observed a tendency to lower parasi
Page 47 and 48: SERUM COMPONENTS AND MONONUCLEAR CE
Page 49 and 50: VIABILITY OF LEISHMANIA (L.) CHAGAS
Page 51 and 52: EVALUATION OF THE INTERFERENCE OF S
Page 53 and 54: IMMUNODETECTION OF THE TGF- β IN L
Page 55 and 56: DIFFERENT LEVELS OF NKT AND NK CELL
Page 57 and 58: Funding: CNPQ; FAPESPA; SESPA; UFPA
Page 59 and 60: saliva that exacerbated leishmania
Page 61 and 62: BACTERIAL FILAMENTATION: SUPER-RESI
Page 63 and 64: IMMUNOLOGICAL PARAMETERS ASSOCIATED
Page 65 and 66: supernatant. BMDC stimulation with
Page 67 and 68: such as number of eosinophils, prot
Page 69 and 70: EVALUATION OF THE PRODUCTION OF TNF
Page 71 and 72: Critical motifs of the Anaplasma ma
Page 73 and 74: SCHISTOSOMA SPP ANTIGENS IMPAIR THE
Page 75 and 76:
DOWN-REGULATION OF IFN-g RECEPTOR A
Page 77 and 78:
MONOCYTES SUBSETS IN SCHISTOSOMIASI
Page 79 and 80:
THE IN VITRO ANALYSIS OF iNOS-KO MO
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CCR7 IS CRITICAL FOR RESISTANCE AGA
Page 83 and 84:
EVALUATION OF THE IMMUNE RESPONSE A
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our data suggest that monocyte secr
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DIVERGENT OUTCOMES OF THE INTERACTI
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they can deactivate immune effector
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Conclusions: We observed a signific
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TLR2 AND TLR4 EXPRESSION AND NUTRIT
Page 95 and 96:
DEVELOPMENT OF MURINE PLACENTAL MAL
Page 97 and 98:
INDUCTION OF TH17 LYMPHOCYTES CONTR
Page 99 and 100:
Leishmania amazonensis INCREASES EC
Page 101 and 102:
TYPE II GRANULOMA INDUCED BY SCHIST
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The Role of Eosinophils in Aspergil
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compared to baseline (p
Page 107 and 108:
disordered loop and NcSAG2A present
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Conclusion: We demonstrate the exis
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WT. By contrast, uninfected AhR -/-
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in the synthesis of proinflammatory
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PRODUCTION AND EVALUATION OF CHICKE
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allow a conclusion about the differ
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contributes to parasite control and
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Financial support: FAPESP, CNPq, an
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MILTEFOSINE EXERTS ITS LEISHMANICID
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IMMUNOMODULATORY ACTIVITIES OF Β-G
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REGULATION OF IMMUNE RESPONSE AGAIN
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THE ROLE OF REACTIVE OXIGEN SPECIES
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INNATE AND ADAPTIVE IMMUNE REPONSE
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KINETIC OF THE SPECIFIC HUMMORAL IM
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PURINERGIC RECEPTOR P2Y12 ACTIVATIO
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Evaluation of cytokine and chemokin
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Financial support: This research wa
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COMPARISON OF VIRULENCE AND IMMUNE
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THE IMPACT OF TUBERCULOSIS IN THE I
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GALACTOMANNAN ANTIGENEMIA SCREENING
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NATURAL ISOTYPIC RESPONSE AGAINST P
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Characterization of costimulatory m
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P2X7 RECEPTOR ACTIVATION IS REQUIRE
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CASPASE-1 AND NLRP3 CONTRIBUTE TO T
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circulating Treg cells expressing C
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PBMC, none of the treatments alter
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CHARACTERIZATION OF MONOCYTE SUBSET
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EFFECT OF OUABAIN IN MACROPHAGES IN
Page 163 and 164:
OspC1 AND OspF: VIRULENCE FACTORS I
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Conclusion: Taken together, these r
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FIB produced higher proportion of f
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MØ phenotype M2, resulting in decr
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Financial support FAPESP, CAPES, CN
Page 173 and 174:
ALTERATION IN THE DISTRIBUTION OF P
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BENEFICIAL EFFECTS OF PENTOXIFYLLIN
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MODULATION OF IMMUNE RESPONSE AND C
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FAS/FASL AND TRAIL CAUSE APOPTOSIS
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actin were more frequently detected
Page 183 and 184:
NEUTROPHILS AND MACROPHAGES ARE INV
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EXPLORING THE INFLAMMATORY ROLE OF
Page 187 and 188:
CERVICAL LEVELS OF INTERLEUKIN-1 BE
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Title: IDENTIFICATION BY PHAGE DISP
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PROFILE OF PRO- AND ANTI-INFLAMMATO
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IMMUNOMODULATORY MECHANISMS OF SOLU
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EVALUATION OF THE TH1, TH2 AND TH17
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production, as the blockade of PD-L
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CHARACTERIZATION OF POLYMORPHISM TN
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seems to be dependent on M. leprae
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7 with tendency to normalization on
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CHARACTERIZATION OF THE CONSERVATIO
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ASC INFLAMMASOME IS NECESSARY TO AC
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CASPASE-1 IS REQUIRED TO CONTROL OF
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The role of CD4 + T lymphocytes dur
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CRITICAL ROLE OF MYD88 EXPRESSION I
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on ROS, cathepsin B and Syk kinase
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Financial Support: FAPESP and CNPq.
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80% in symptomatic group; 45% in as
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of cells undergoing apoptosis in DT
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Financial support: FAPESP/LIM-56/HC
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identify the active components and
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EVALUATION OF HUMORAL AND CELULLAR
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Financial support: CNPq-PIBIC, FAPE
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addition, significant expression of
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THE ROLE OF K + TRANSPORTERS IN THE
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Conclusion: In this study, we estab
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Heme activates oxidative mechanisms
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ROLE OF ADENOSINE AND PROSTAGLANDIN
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Taken together, our results indicat
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Conclusion: Our findings suggest th
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Financial support: CNPq, CAPES, IOC
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number of circulating parasites in
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CRITICAL ROLE OF IL-6 IN REGULATORY
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PPAR-α AGONIST GEMFIBROZIL DECREAS
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Cutaneous Leishmaniasis in Amazonas
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CYTOTOXICITY IN IMMUNOPATHOGENESIS
Page 257 and 258:
ROS production and TLR2 and 4 expre
Page 259 and 260:
ROLE OF IL-4 IN THE INTESTINAL INFL
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In vitro CULTURE SYSTEM FOR Plasmod
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interactions, Tandem Affinity Purif
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in the course of human infection wi
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findings suggest that IL-17 and IL-
Page 269 and 270:
23 contribute to immunological cont
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not produced during infection by C.
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Conclusion: The production of cytok
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CONCLUSION: It is likely, in this p
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expression in CCC myocardium was 64
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that mononuclear cells derived from
Page 281 and 282:
Conclusion: Altogether, our data sh
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PARACOCCIDIOIDES BRASILIENSIS INHIB
Page 285 and 286:
CANINE VISCERAL LEISHMANIASIS AND S
Page 287 and 288:
IMMUNOMODULATORY EFFECTS OF BIOTHER
Page 289 and 290:
Leishmania EFFECT ON HEME CYTOTOXIC
Page 291 and 292:
ROLE OF STRONGYLOIDES VENEZUELENSIS
Page 293 and 294:
TREATMENT WITH Harpagophytum procum
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