immunology of infectious and parasitic diseases - XXXVII Congress ...

EVALUATION OF HUMORAL AND CELULLAR IMUNE RESPONSE OF
SHEEP TO ANTIGENS OF CORYNEBACTERIUM PSEUDOTUBERCULOSIS
TREATED WITH AQUEOUS EXTRACT OF CAESALPINIA PYRAMIDALIS
SANTANA, MARTA MARIA OLIVEIRA 1 ; MEYER, ROBERTO 5 ; LIMA,
DANIELLE DANTAS 5 ; DAVID, JORGE MAURÍCIO 3 ; DAVID, JUCENI
PEREIRA 2 ; ZACHARIAS, FAROUK 4 ; OLIVEIRA, RICARDO RICCIO 2 ; LIMA,
FERNANDA WASHINGTON DE MENDONÇA 2 .
1 UFBA, Escola de Medicina Veterinária e Zootecnia, Salvador - BA, Brasil.
2 UFBA, Faculdade de Farmácia, Salvador - BA, Brasil;
3 UFBA, Instituto de Química, Salvador - BA, Brasil;
4 Empresa Baiana de Desenvolvimento Agrícola, Salvador - BA, Brasil
5 UFBA, Instituto de Ciências da Saúde, Salvador - BA, Brasil
Introduction: Many popular plant extracts seem to stimulate cellular and
humoral immunity in animals. This study evaluated the effect of an aqueous
extract of Caesalpinia pyramidalis on the immune response of sheep
experimentally stimulated with Corynebacterium pseudotuberculosis.
Methods and Results: The study included 20 sheep of both gender that were
90 and 270 days of age and serologically negative for C. pseudotuberculosis.
The sheep were divided into four experimental groups: G1 was the control, G2
received treatment with extract of C. pyramidalis, G3 received an extract of C.
pyramidalis associated with Antigen MQD and G4 was treated with Antigen
MQD. After antigenic stimulation, blood was collected for serology during eight
months. The specific cellular and humoral immune responses against C.
pseudotuberculosis were assessed by specific IgG production and IFN-γ,
respectively. G2 presented the highest IgG values 120 (O. D. 0,2498) days after
treatment. G3 showed higher values than all other groups after 90 (O. D.
0,9582), 120 (O. D. 0,7638) and 180 (O. D. 0,8383) days and G4 showed its
greatest values after 150 (O. D. 0,7685) days. G2 and G3 displayed the
greatest values of IFN-γ 120 (O. D. 257,8 and 691,8) days after treatment (p<
0.05), while the greatest values in the G4 group were obtained 150 (O. D.
723,0) days after stimulation.
Conclusion: The extract of C. pyramidalis seemed to promote the stimulation
of the immune responses; however, other studies are needed to investigate its
mechanisms of action.
Financial support: BNB-FUNDECI