immunology of infectious and parasitic diseases - XXXVII Congress ...

NEUTROPHILS AND MACROPHAGES ARE INVOLVED IN THE
PATHOGENESIS OF MALARIA ASSOCIATED ACUTE LUNG
INJURY/ACUTE RESPIRATORY DISTRESS SYNDROME IN A MURINE
MODEL
LUANA DOS SANTOS ORTOLAN 1* ; MICHELLE KLEIN SERCUNDES 2* ;
DANIELA DEBONE 2 ; ELIZABETH HELEN AITKEN 3 ; MOMTCHILO RUSSO 3 ;
JOSÉ M. ALVAREZ 3 ; CLAUDIO ROMERO FARIAS MARINHO 4 AND
SABRINA EPIPHANIO 1,2,3
1 Departamento de Ciências Biológicas, Universidade Federal de São Paulo,
Diadema, Brazil
2 Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São
Paulo, Brazil
3 Departamento de Imunologia, Universidade de São Paulo, São Paulo, Brazil
4 Departamento de Parasitologia, Universidade de São Paulo, São Paulo, Brazil
* These authors contributed equally to this work.
INTRODUCTION: Malaria is a huge burden on global health. In 2010, this
parasitic disease was the underlying cause of death for 1,24 million individuals.
Severe malaria can manifest in the lungs, an illness known as acute lung
injury/acute respiratory distress syndrome (ALI/ARDS). We previously
established a murine model that mimics various human ALI/ARDS aspects,
such as pulmonary edema, hemorrhages, pleural effusion and hypoxemia.
Using this model we have evaluated the cellular immune profile as well as other
aspects of the immune response to investigate the possible role of the
inflammatory response in the pathogenesis of malaria associated ALI/ARDS.
METHODS AND RESULTS: DBA/2 mice were infected with Plasmodium
berghei ANKA. Lungs and bronchoalveolar lavage were collected and analyzed
at various time-points during infection. By using the parameters respiratory
pattern (enhanced pause and respiratory frequency) and parasitemia from a
control group (our gold standard survival group), we established ROC curves
and were able to classify experimental animals euthanized at the different timepoints
as suffering ALI/ARDS or HP (hyperparasitemia). Levels of 18s