immunology of infectious and parasitic diseases - XXXVII Congress ...

EVALUATION OF THE TH1, TH2 AND TH17 CYTOKINES LEVEL IN HCV +
PATIENTS ATTENDED IN THE FUNDAÇÃO DE MEDICINA TROPICAL OF
AMAZONAS - BRAZIL
ADRIANA MALHEIRO(PG) (1,4) ; ANDRÉA MONTEIRO TARRAGÔ(PG) (1,4) ;
RAPHAEL ALLE MARIE (2) ; ALLYSON GUIMARÃES DA COSTA(PG) (1,4) ; JOÃO
PAULO DINIZ PIMENTEL(PG) (3,4) ; ANA RUTH ARAÚJO(PG) (1,2) ; MARILÚ
VICTORIA(PG) (2) ; FLAMIR VICTORIA(PG) (2) .
(1). Universidade Federal do Amazonas; (2).Fundação de Medicina Tropical do
Amazonas; (3).Fundação Oswaldo Cruz; (4). Fundação de Hematologia e
Hemoterapia do Amazonas Universidade Federal do Amazonas.
Introduction: Hepatitis C infection is a major cause of acute and chronic
hepatitis. It is estimated that 170 million people worldwide are infected by virus
C. Most of infected individuals are not able to clear the virus and may
progresses to cirrhosis or hepatocelular carcinoma. The immune responses
play an important role in both HCV clearance and persistence of the infection.
This study aimed to evaluate the cytokines level of HCV + patients untreated with
ribavirin and interferon alpha and assisted at Fundação de Medicina Tropical of
Amazonas. Methods and Results: The concentration of IL-2, IL-4, IL-6, IL-10,
TNF-α, IFN-γ and IL17A were performed by flow cytometry using the kit TH1,
TH2 and TH17 (BD cytometric Bead Array (CBA) Human Cytokine
Th1/Th2/Th17 Kit) following the manufacturer's technical guidelines. The results
were analyzed using the statistical software GraphPad Prism® and the test
applied was Mann Whitney test. The sample consists of 74 HCV + patients and
47 controls. When we compared the HCV + patients with the control group the
results were statistically significant for IL-2 (p˂ 0, 0001), IL-6 (p˂0,0001), IL-10
(p˂0,0001), IFN (p˂ 0,0001), TNF (p=0,0061) e IL17 (p˂0,0001). On the other
hand, the IL-4 level (p= 0, 1511) was not statistically significant. Conclusion:
We conclude that HCV infection induces the Th1 and Th17 profiles
simultaneously overlapping the Th2. However, further analysis should be
conducted to better understand the interaction of the virus studied and know the
immunological profile of HCV + patients.
Financial Support: FAPEAM,CAPES, CNPq.
Key word: Cytokines, Hepatitis C, Th1, Th2, Th17.