immunology of infectious and parasitic diseases - XXXVII Congress ...

TLR2 AND TLR4 EXPRESSION AND NUTRITIONAL PROFILE IN PATIENTS
WITH VISCERAL LEISHMANIASIS. PRELIMINARY RESULTS.
MARIANA GATTO 1 ; MARIANA MIZIARA DE ABREU 1 ; MARJORIE DE ASSIS
GOLIM 2 ; LORAINE GOLLINO 1 ; JOSÉ CLÁUDIO SIMÃO 3 ; KAREN INGRID
TASCA 1 ; CARLOS MAGNO CASTELO BRANCO FORTALEZA 1 ; PAULO
CÂMARA MARQUES PEREIRA 1 ; SUELI APARECIDA CALVI 1
1 Departament of Tropical Diseases, Botucatu School of Medicine - UNESP, São
Paulo State University, Brazil; 2 Flow Citometry Laboratory, Hemocenter,
Botucatu School of Medicine - UNESP, São Paulo State University, Brazil;
3 Manoel de Abreu Hospital, Bauru, São Paulo, Brazil.
Introduction: Visceral leishmaniasis is an emerging public health problem, with
500,000 new cases per year. In order to have the development of adaptive
immune response is necessary that cellular receptors such as TLR2 and TLR4
recognize and play the parasite effector mechanisms. Moreover, the nutritional
profile also has impact on immune response. Our objective was to evaluate the
TLR2 and TLR4 expression and correlate with the nutritional profile of patients
with visceral leishmaniasis before and after treatment. Methods and results:
Were evaluated 8 patients pre-treatment, 3 post-treatment and 8 healthy
subjects. Analysis of the expression of TLR2 and TLR4 on CD3 and CD14 cells
was performed by flow citometry and the nutritional profile was performed by
bioelectrical impedance and biochemical tests. In CD3 cells, the percentage of
TLR2 expression was higher in patients pre-treatment (36,9±14,5) than those
post-treatment (5,7±5) and controls (1,42±1,03); percentage of TLR4
expression, although low in all groups, was higher in pre-treatment (2,8±1,4)
compared to controls (1±0,3). Percentage of TLR2/TLR4 coexpression in CD3
cells was higher in pre-treatment (2,4±1) compared to controls (1±0,1).
Regarding to CD14 cells, percentage of TLR2 expression was lower in pretreatment
(83,7±13,6) than those controls (98,9±0,8); percentage of TLR4
expression and TLR2/TLR4 coexpression did not differ between groups. The
percentage of body fat was lower in patients pre-treatment (16,3±10,6)
compared to controls (27,4±6,3); phase angle was lower in patients pretreatment
(5.8º±1,3) compared to pos-treatment (7.0°±0,4). HDL and albumin
concentrations were lower in pre-treatment (9,8±11,9 e 2,2±0,6) than those
post-treatment (41,5±6,3 e 4,2±0,05) and controls (65,8±13,4 e 4,3±0,4)
respectively; LDL and total cholesterol levels in pre-treatment (101,4±30,6 e
58±17,9) were lower than in controls (199±22,5 e 113,32±24,3) respectively.
Conclusion: Our results suggest that patients with active LV expressed TLR2
and TLR4 in monocytes and lymphocytes, suggesting a possible involvement of
these receptors in the development of the immune response. Patients also had
nutritional profile changes that may be interfering with the immune response
against the parasite.