immunology of infectious and parasitic diseases - XXXVII Congress ...

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Pseudomonas aeruginosa 1-Cys Peroxiredoxin LsfA is involved in virulence GILBERTO HIDEO KAIHAMI (PG) (1) ; SUELEN SILVANA DOS SANTOS (PG) (2) ; JOSÉ ROBERTO FOGAÇA DE ALMEIDA (PG) (2) ; SANDRO ROGÉRIO DE ALMEIDA (2) ; REGINA LÚCIA BALDINI (1) (1) INSTITUTO DE QUÍMICA – USP (2) FACULDADE DE CIÊNCIAS FARMACÊUTICAS – USP Introduction: Bacteria are recognized by macrophages via Toll-Like Receptors (TLR), leading to a signaling pathway that activates NF-kB and MAPKs and killing in phagossomes in macrophages is achieved by reactive oxygen and nitrogen species (ROS/RNS) generation. P. aeruginosa is a common cause of ventilator associated pneumonia and it uses several strategies for virulence and defense, including antioxidant mechanisms. In this work, we show for the first time that the 1-Cys peroxiredoxin, LsfA, is implicated in bacterial virulence. Methods and Results: Mutant strains with a deletion in lsfA or a C45A mutation were constructed and they were more sensitive to H2O2 than the wild type strain PA14, as measured in a halo inhibition assay. In vitro peroxidasic activity of LsfA was measured by ferric-thiocyanate assay, and while the wildtype protein was active, the mutation in Cys45 abolished its activity. Infection of J774 macrophages with DLsfA or C45A strains resulted in lower cell death, increased bacterial clearance and higher TNF-a in comparison to PA14-infected macrophages, suggesting a higher level of MAPKs and NF-kB activation in macrophages infected with the mutants strains. To verify if LsfA could modify the oxidative state of infected macrophages, they were infected with PA14 and C45A strains and incubated with carboxy-H2DCFDA. Macrophages infected with mutant strains showed higher oxidative state in comparison to PA14infected cells, thus confirming that LsfA limits macrophages activation that lead to TNF-a production and cytotoxic activity. MAPKs and NF-kB pathways are required to full production of TNF-a in macrophages infected with C45A, as shown using pharmacological inhibitors for those pathways. When macrophages were infected with C45A in the presence of the antioxidant NAC, there was a reduction in TNF-a production as compared to PA14, as expected. In an acute pneumonia model, all PA14-infected mice died at 48h p.i., while C45A-infected mice survived as long as 60 days after infection. There was also CFU reduction in the lungs, spleen and liver of mice infected with C45A, in comparison to PA14-infected mice.
Conclusion: Altogether, our data show for the first time the role of a bacterial 1- Cys Prx (LsfA) to modulate host immune response in vitro and in vivo. Financial Support: FAPESP, CNPq
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IMMUNOLOGY OF INFECTIOUS AND PARASI
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profiles appear to be dependent on
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INVOLVEMENT OF TNF RECEPTORS IN APO
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Effects of bioactive Cryptococcus n
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DCs expressing Indoleamine 2,3-diox
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DETECTION OF GITR ON PATIENT CERVIX
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DETECTION OF GITR AND IL-10 ON CERV
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VACCINATION WITH SM10.3 ANTIGEN, A
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Financial support: FAPESP, CAPES an
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clearance that follows Sylvio X10/4
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EhMSP-1 - AN ENTAMOEBA HISTOLYTICA
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THERAPY WITH SCFV TRANSFECTED-DENDR
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etween the groups. The results show
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Financial support: FIOCRUZ, CNPq.
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elease of NETs from human neutrophi
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ROLE OF CD18 IN ACTIVATION OF M1 MA
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IDENTIFICATION, PURIFICATION AND CH
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with hemin showed higher levels of
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SEPTIC ARTHRITIS TRIGGERED BY S. au
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eing higher levels induced by a vir
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parasite load skin. The TGF-β was
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cultures, both treatments decreased
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observed a tendency to lower parasi
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SERUM COMPONENTS AND MONONUCLEAR CE
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VIABILITY OF LEISHMANIA (L.) CHAGAS
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EVALUATION OF THE INTERFERENCE OF S
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IMMUNODETECTION OF THE TGF- β IN L
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DIFFERENT LEVELS OF NKT AND NK CELL
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Funding: CNPQ; FAPESPA; SESPA; UFPA
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saliva that exacerbated leishmania
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BACTERIAL FILAMENTATION: SUPER-RESI
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IMMUNOLOGICAL PARAMETERS ASSOCIATED
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supernatant. BMDC stimulation with
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such as number of eosinophils, prot
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EVALUATION OF THE PRODUCTION OF TNF
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Critical motifs of the Anaplasma ma
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SCHISTOSOMA SPP ANTIGENS IMPAIR THE
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DOWN-REGULATION OF IFN-g RECEPTOR A
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MONOCYTES SUBSETS IN SCHISTOSOMIASI
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THE IN VITRO ANALYSIS OF iNOS-KO MO
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CCR7 IS CRITICAL FOR RESISTANCE AGA
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EVALUATION OF THE IMMUNE RESPONSE A
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our data suggest that monocyte secr
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DIVERGENT OUTCOMES OF THE INTERACTI
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they can deactivate immune effector
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Conclusions: We observed a signific
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TLR2 AND TLR4 EXPRESSION AND NUTRIT
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DEVELOPMENT OF MURINE PLACENTAL MAL
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INDUCTION OF TH17 LYMPHOCYTES CONTR
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Leishmania amazonensis INCREASES EC
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TYPE II GRANULOMA INDUCED BY SCHIST
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The Role of Eosinophils in Aspergil
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compared to baseline (p
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disordered loop and NcSAG2A present
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Conclusion: We demonstrate the exis
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WT. By contrast, uninfected AhR -/-
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in the synthesis of proinflammatory
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PRODUCTION AND EVALUATION OF CHICKE
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allow a conclusion about the differ
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contributes to parasite control and
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Financial support: FAPESP, CNPq, an
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MILTEFOSINE EXERTS ITS LEISHMANICID
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IMMUNOMODULATORY ACTIVITIES OF Β-G
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REGULATION OF IMMUNE RESPONSE AGAIN
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THE ROLE OF REACTIVE OXIGEN SPECIES
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INNATE AND ADAPTIVE IMMUNE REPONSE
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KINETIC OF THE SPECIFIC HUMMORAL IM
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PURINERGIC RECEPTOR P2Y12 ACTIVATIO
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Evaluation of cytokine and chemokin
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Financial support: This research wa
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COMPARISON OF VIRULENCE AND IMMUNE
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THE IMPACT OF TUBERCULOSIS IN THE I
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GALACTOMANNAN ANTIGENEMIA SCREENING
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NATURAL ISOTYPIC RESPONSE AGAINST P
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Characterization of costimulatory m
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P2X7 RECEPTOR ACTIVATION IS REQUIRE
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CASPASE-1 AND NLRP3 CONTRIBUTE TO T
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circulating Treg cells expressing C
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PBMC, none of the treatments alter
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CHARACTERIZATION OF MONOCYTE SUBSET
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EFFECT OF OUABAIN IN MACROPHAGES IN
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OspC1 AND OspF: VIRULENCE FACTORS I
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Conclusion: Taken together, these r
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FIB produced higher proportion of f
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MØ phenotype M2, resulting in decr
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Financial support FAPESP, CAPES, CN
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ALTERATION IN THE DISTRIBUTION OF P
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BENEFICIAL EFFECTS OF PENTOXIFYLLIN
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MODULATION OF IMMUNE RESPONSE AND C
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FAS/FASL AND TRAIL CAUSE APOPTOSIS
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actin were more frequently detected
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NEUTROPHILS AND MACROPHAGES ARE INV
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EXPLORING THE INFLAMMATORY ROLE OF
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CERVICAL LEVELS OF INTERLEUKIN-1 BE
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Title: IDENTIFICATION BY PHAGE DISP
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PROFILE OF PRO- AND ANTI-INFLAMMATO
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IMMUNOMODULATORY MECHANISMS OF SOLU
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EVALUATION OF THE TH1, TH2 AND TH17
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production, as the blockade of PD-L
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CHARACTERIZATION OF POLYMORPHISM TN
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seems to be dependent on M. leprae
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7 with tendency to normalization on
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CHARACTERIZATION OF THE CONSERVATIO
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ASC INFLAMMASOME IS NECESSARY TO AC
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CASPASE-1 IS REQUIRED TO CONTROL OF
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The role of CD4 + T lymphocytes dur
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CRITICAL ROLE OF MYD88 EXPRESSION I
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on ROS, cathepsin B and Syk kinase
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Financial Support: FAPESP and CNPq.
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80% in symptomatic group; 45% in as
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of cells undergoing apoptosis in DT
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Financial support: FAPESP/LIM-56/HC
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identify the active components and
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EVALUATION OF HUMORAL AND CELULLAR
Page 229 and 230: Financial support: CNPq-PIBIC, FAPE
Page 231 and 232: addition, significant expression of
Page 233 and 234: THE ROLE OF K + TRANSPORTERS IN THE
Page 235 and 236: Conclusion: In this study, we estab
Page 237 and 238: Heme activates oxidative mechanisms
Page 239 and 240: ROLE OF ADENOSINE AND PROSTAGLANDIN
Page 241 and 242: Taken together, our results indicat
Page 243 and 244: Conclusion: Our findings suggest th
Page 245 and 246: Financial support: CNPq, CAPES, IOC
Page 247 and 248: number of circulating parasites in
Page 249 and 250: CRITICAL ROLE OF IL-6 IN REGULATORY
Page 251 and 252: PPAR-α AGONIST GEMFIBROZIL DECREAS
Page 253 and 254: Cutaneous Leishmaniasis in Amazonas
Page 255 and 256: CYTOTOXICITY IN IMMUNOPATHOGENESIS
Page 257 and 258: ROS production and TLR2 and 4 expre
Page 259 and 260: ROLE OF IL-4 IN THE INTESTINAL INFL
Page 261 and 262: In vitro CULTURE SYSTEM FOR Plasmod
Page 263 and 264: interactions, Tandem Affinity Purif
Page 265 and 266: in the course of human infection wi
Page 267 and 268: findings suggest that IL-17 and IL-
Page 269 and 270: 23 contribute to immunological cont
Page 271 and 272: not produced during infection by C.
Page 273 and 274: Conclusion: The production of cytok
Page 275 and 276: CONCLUSION: It is likely, in this p
Page 277 and 278: expression in CCC myocardium was 64
Page 279: that mononuclear cells derived from
Page 283 and 284: PARACOCCIDIOIDES BRASILIENSIS INHIB
Page 285 and 286: CANINE VISCERAL LEISHMANIASIS AND S
Page 287 and 288: IMMUNOMODULATORY EFFECTS OF BIOTHER
Page 289 and 290: Leishmania EFFECT ON HEME CYTOTOXIC
Page 291 and 292: ROLE OF STRONGYLOIDES VENEZUELENSIS
Page 293 and 294: TREATMENT WITH Harpagophytum procum
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