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PHYTOCHEMICAL ANALYSIS Figure 1. HPLC chromatograms of an authentic sample (0.2551Ag) of salvinorin A (A), and of representative Salvia divinorum tissue extracts obtained from "Palatable" clone (Bret Blosser) (B), from Cerro Rab6n clone (L. J. Valdés) (C), and from a seed grown plant DS03 (D. J. Siebert) (D). In each case the retention time of the peak representing salvinorin A is indicated. (For chromatographic protocol see Experimental section). http://www.sagewisdom.org/phytochemical.html (5 of 9) [04.09.01 10:20:46]
PHYTOCHEMICAL ANALYSIS Figure 2. The composite standard calibration for the quantification of salvinorin A by HPLC (the error bars indicate ±1 standard deviation; the linear regression equation for the calibration curve is y= 759,334x •44,127). A composite standard curve for 1 was compiled from three individual determinations of the standard curve (Fig. 2). The correlation coefficients for these three standard curves were 0.9997, 0.9997 and 0.9967. The coefficient of variation among the three curves was 5.9%. The coefficients of variation for each injected amount across all three days ranged from 1.7 to 5.7%. The peak identified as salvinorin A (1) from authentic standard solutions was observed in extracts of leaves and stem from S. divinorum. In order to examine the concentration range of 1 found in various populations of S. divinorum, 20 different samples of leaves were collected from cultivated plants in private collections as well as endemic populations of the plant in Oaxaca. Representative chromatograms for three different samples of S. divinorum are shown in Fig. 1 Estimations were based on the average of two separate injections. The determinations of the leaf content of salvinorin A showed a broad range of values ranging from 0.89 to 3.70 mg/g dry weight (Table 1). Stem material showed considerably lower values of 1 equivalent to approximately 4% of the level found in leaves. http://www.sagewisdom.org/phytochemical.html (6 of 9) [04.09.01 10:20:46]
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The Salvia divinorum Research and I
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Daniel J Siebert's Home Page analog
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type_Document_Title_here A Salvia d
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Molecular Model of Salvinorin A Sal
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type_Document_Title_here The coroll
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The Salvia divinorum FAQ I. ACKNOWL
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LE GUIDE D'UTILISATION DE LA SALVIA
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LA GUIA DE USUARIOS DE SALVIA DIVIN
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Salvinorin Salvinorin, A New trans-
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Salvinorin [Arachnid note - Tables
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DivinorinJOC1984 Figure 1. Divinori
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DivinorinJOC1984 anhydride/pyridine
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DivinorinJOC1984 Matthaei Botanical
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loliolide LITERATURE CITED 1. L.J.
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ecbotsdiv Studies of Salvia divinor
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ecbotsdiv Beltrán, claiming that p
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ecbotsdiv Although all recent infor
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ecbotsdiv Urbina, M. 1903. El peyot
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Ps94amay99 Salvinorin A (1) Salvino
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Salvia divinorum photos from L. J.
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(stereochem.) The Absolute Stereoch
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(stereochem.) assignable to the lon
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D.M. Turner's SALVINORIN Book Infor
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ABSTRACT leaves have an opposite ar
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