Environmental and Molecular Mutagenesis - Legacy Tobacco ...

150 1989 EMS Abstracts - -~~ ._ - . s~
http://legacy.library.ucsf.edu/tid/clb93d00/pdf
Notes umols/plate in tha .presence or absence of S9 . TREO is knovn to hydrolyse to
DES and MSA vf tlr~lsaasing pH . TREO mutagenicity vas tested at pH 6 .0, 7 .0,
and 8 .0 and shovn to be p8-dependent, vith the highest autagenic response and
the least toxicity seen at pH 6 .0 ; the lovest response and the greatest
toxicity vas seen at pH 8 .0 . DEB mutagenicity vas unaffected by pH, and its
autagenicity patterns vere similar to TREO at pH 8 .0 . At this pH, the
hydrolysis of TREO to DEB is rapid . It vould be expected that the
mutagenicity of DEB, rapidly produced from TREO (at pH 8 .0), vould be similar
to DEB directly applied . The nonmutagenicity of NSA eliminates it from
consideration as the active hydrolysis product . These results are consistent
vith the conclusion that TREO is mutagenic through its nonenzyaatic hydrolysis
to DEB .
434
BIOLOGICAL AND ANALYTICAL MONITORING OF RIVER WATER POLLUTION IN CAMPANIA, ITALY .
G .Pagano, G .Melluso ; G .Corsale, A .Esposito, M .Ouida: and G .G .Giordano, Ystituto
Nazionale Tumori, 1-80131 Naples, and * Dipartimento di Fisiologia Generale ed
Ambientale, Facoltg di Scienze . Universitl di Napoli, 1-80134 Naples, Italy
Several emissions of industrial, agricultural, and domestic sewage affect the Sarno
river and the drainage system of the Volturno river-Regi Lagni in the Campania region,
Italy . The possible consequences on marine biota near the mouths of the two rivers
and the Regi Lagni have been investigated by biweekly sampling of river water from ten
sites ; some experiments were performed by daily or circadian sampling from two selec-
ted sitea . Biological monitoring was performed by testing river water samples diluted
in seawater (10- to 10-') on sea urchin embryos and sperm, and scoring the outcomes
in terms of : a) developmental toxicity ; b) fertilization success ; c) cytogenetic ab-
normalities . Chemical analysis was carried out on water and sediment, and included
several pollution parameters, as : i . COD and BOD, ; ii . NO=, N0„ and NH ; ; iii . some
selected inorganice [Cd(II) ; Cr(III) and (VI) ; As(III) and (V)] ; iv . some selected
organics (PAH's, PCB's, some pesticides) . The results showed : 1) the presence of re-
markable pollution and toxicity at the mouths of the Sarno river and the Regi Lagni,
whereas the Volturno river resulted to be less affected ; 2) daily and circadian
changes of pollutant levels ; 3) a good agreement of biological vs . analytical data .
(Partly supported by WHO, and by the Italian Ministry of Health) .
435
FANCONI'S ANEMIA (FA) LYMPHOBLASTS BELONGING TO COMPIEMENTATICN GROUPS
"A" AND "B" ARE HYPOMUTABLE FOLLOWING TREATMENT WITH PHOTOACTIVATED
PSORALENS . D . Pa ad oulo, B . Porfirio and E . Moustacchi . Institut Curie,
Section de B o og e, UA 1292 CNRS, 26 rue d'Ulm, 75231 Paris cedex 05,
France .
Fanconi's anemia (FA), an eutoscmal•recessive disease, beldngs to the
group of human genetic disorders cha;acterized by chranosaaal instability
and a predisposition to malignancy . At the cellular level, FA cells
are hypersensitive to DNA cross-linking agents, a feature likely to be
related to a defect in the processing of lesions induced by these
agents . Two genetic complementatien groups have been recently described
"A" and "B" . We have previously demonstrated that FA group "A" cells
are sensitive to cross-links and monoadducts (MA) induced by photoactivated
bifunctional psoralens, whereas FA group "B" cells are eapecially
sensitive to MA induced by these agents . In the present study, we measured
in normal and FA lymphoblasts the frequency of 6TGr and ouar mutations
induced by 8-methoxypsoralen or trimethyl,4,5',8,psoralen in the
presence of 365 nm radiation . We observed that FA cell lines fraa both
complementation groups are clearly hypaoutable for the two loci considered
. The FA group "A" cells are almost immutable . The molecular analysis
of the spectrum of HGPRT- mutants induced by photoactivated psoralens is
in progress .
436
FISH CELL LINE DEB.IVEJ FROlf THE FIN OF TBE CEN(RAL MUDHINJOM/ (UMBRA L=) : A SUITABLE
MC)DEL FOR CLASTOGENICITY ASSAY . E .-H . Park, J .-S . Lee, A .-K. Yi and H . Etch,
Department of Biology, College of Sciences, Hanyang University, Seoul 133-791 (Korea),
and Division of Biology, National Institute of Radiological Sciences, Chiba 260 (Japan)
A cell line (ULF-23HU) from the fin of the central madminnow (UMbrA UML) was
characterized and tested for its suitability to aseess cytogenetic damages induced by
chemicals in fish . . Cells of this line exhibit a fibroblaat-like appearance and grew
50869 3664