Environmental and Molecular Mutagenesis - Legacy Tobacco ...

92 1989 EMS Abstracts
Notes
http://legacy.library.ucsf.edu/tid/clb93d00/pdf
262
SHURT-:r9Ji TES"C FCR FRF:DICTICN OF CARCINUGF24S AND/UR YAU11UT8RS
?(uanq Fianjun, S .M .Chen and F .Lin
Nat . S.ar.t . Contr . Phara . & Biol . Prod . lseijing, China
e T.a7sr7 sch9me of mutagrnicity has boen established for investigation of thirty
Eninaa.e herbs and pbarmaceutieals . Tnroe amrays applied were within the schemes
c :,.te :tinle gene mutation in Awes tsntt detent:ng ohroa,oso .al aberration in CHL
(Ch :nese hamster fibroblast cell line) teat :n vitro and in oiaronuoleus in vivo .
Sy aix nas been applied in vitro . Mutaganioity of unknovn drugs in CBL teat, 37 .5%
druge (9/24) induc-d chromosomal aberrations, 4•17yi drugs (1/24) shown inconcluaive
(sle 58 .3% drugs (14/24) didn't induce chro®osoeal aberrations . In aieronueleus
teatn, V,3% drup,e (6/2 ;) induced mioronuoleuted yolychrooatie erythroeytes (MNYCE)
incrvr :ng, 73 .5% dritn (,17/23) shown Y.N" vas in nosmal . in /wea teats, the ∎utation
nus-~bere of 4 strains varied within nosmal fur 24 drup.e . In wutagenicity study on 30
druf*s, cytogenetic study has ehom that suta&nr, and/or caroinogens induoed
chroc!osowal aberrations in mammalian eells in CHL and wioronuelsue tests . Howver,
mutap+ns and/or carcinogens dldn't induee gene autation in AsNa testa, especially,
horm,ne and antinotaboliea druRe . The battery ecneote of mutagenioity, especially,
:wong GNL and mloronuoloua test, hae been prov .d being effeotive, sensitive, accurate
and rpecific, but, Ames test seem *neffective, unaensitive and tnacourste .
263
MV:ACFaS 111 DRUGS
Na.ang Nianjun, J,it .Neng, S .ll,Chen and D .q .bi
Nat . Snst, Contr . Thans, & Biol . Prod . ]bijing, China
an our elrtwg+nicity study on thirty drugs, cytogenetio study has shown that
mute,w9ca arxl/or caroinogens induced chroaosomal aberrations in sesuealian oells in
C3IL and sicronucleus teste . Nine drugs have been proved to be sutagsns . Among nine
auta."•-is, one indirect mutagens and eight direct sutagene and/or promoters were
discovered . 8561 (Coumarin from Ruta, graveoleus L), no ohromososal aberrations in
C~:;, in culture when the cells vere directly treated for 24h and 48 h . While
chror.ozenal aba*rations wers ∎ore prominently with S9 six and showed clearly dosedepenier.t
relation . Tr^ break and exchange at .errations reached at 93% and 69%
(72 . ;nc,/nl) respectively . Folloving five airt-ot .utsgena induced chromoaomal
s.berritions withcut 69 mix and also showed the dose-dependent relation . The rate of
c5roonaosal aberrations and nieronueleated Yolychromatio erythrocytes in Canciolovir
vne ?t& (0 .5u ./al) and 16.67J4 . (Bi~Ong/kr'), iiarrsngtonine 47% (o .1y5ug/al) and 18 .05xe
10 .4j :ng/Ir.g), Boanharringtonine 25% (n .1y5ug/v:i and 10 .940 (0 .625mg/kg),Desciclwir
+1% (2 . ;?mt;/m1) and 10 .5%e (50W'g/kg), and Sulbactum NV)L (4 .59®g/ml) and 14 .9% .
(± .04r:kg) . AnQther three drugs induc-d polyploid aberrations in CHL test,The rat .e
of po::yplo.id ar,orrations for three druo reached very high levels Flubandosole 95%
ea 0 .?bug./vl, 8stinylestrsdial 8YY. at 12 .5ug/sl, Hezoesterol 40A at 15ug/ml, The
ei£~e :i .e to ind-n po2yploid aberrntiooc wan like dlnthyl,tilbestral .
264
TI$ $BFfiCT OF HR7fACHLOR09@iZ6NE AND DDT ON RfiPlODIQCTIPi OUT00lS :4 OF RURAL M0lRN.
X . HUARG, S . VANG, X . sAN, BNTIRCIIlWlAL 1MAL'!8 I118TI1111Ti, ZtVI4liC lEDICAL DPIYSRSITY
,RANGZHOa, CBINA
It was founded that 11BC and DDT levels in paddy field soil,surfaoe water and foods
in xizin village dropped rapidly after stopping use of these pesticides at the end of
1982 . The present study was to investigate whether the reproductive outoors of vos»n
in the sera wre related to the use of IdiO and DDT . During 1981-1986 a total of 995
pairs of mother and child tnre underwent medical surveilanoe . 'me detailed questionnaire
and examination mainly concerned rternal reproductive events and congenital aalforsrtione
. The paraosters adopted in the investieation weret inoidenoes of spontaneous
abortione,of gestation period ( 37 weeks or > 42 veska,of neonate birth wight < 2500 g,
congenital malfor .ations and perinatal deaths. 'me results obtained in 1981 end 1982
while llHC and DDT were being used didn't differ statistically from those in 1983-1986
while ZXC and DDT had been banned(P> 0 .56) . 4fter l'41C and DDT vas banned wC level in
breast milk of the village woman dropped fso∎ 0 .8639 pps W982 to 0 .5671 ppa in 1985
and DDT from 0 .4279 ppm in 1982 to 0 .0731 ppn in 1985 Hspeotitnly, Fnrthesmore,regreseion
lines analyses between each inoidence of the abov* reproduotive events in the area
and 1qiC and DDT levels in breast milk in the same year were conducted respeotively . No
signifioant difference was found . 'lhat is to say,the inoidences of abnorrl reproduotive
outooss in Xi=in village remained the sar level after the reduction of ths
. lkiC and conclusion iand abnotr~l this
avestigatioxsi is that no association between the~ use of
reproductive outoomss in Lixin village vas observed .