Environmental and Molecular Mutagenesis - Legacy Tobacco ...

transformation . The BALB/c 3T3 clone A31-1-13 cells were exposed to coal dust
extract for 72 hrs . At th:--end of treatment, the coal dust extract was removed, and
cells were cultivated for 4 weeks with twice weekly medium change . Type III foci
were scored after fixation and staining . Results showed that coal dust extracts,
both nitrosated and nonnitrosated, induced eell transformation in a dose response
manner . However, the transformation frequency was much higher with the nitrosated
than the nonnitrosated coal dust extracts . All transformed eell lines derived from
coal dust extract-induced foci showed characteristics of malignant transformation .
The transforming activity of coal dust extract decreased when chlorophyllin was
incorporated into the treatment solution .--These results seem to support the coal
mine dust hypothesis for the causation of gastric cancer in coal miners and indicate
that chlorophyllin possesses an antitransformation activity .
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RE6P:ARCiI ON MUTAGENICITY OF POLLUT'r.D WATER TREAT6IENT WITH FIOLOGICAL OXIDATION
:+u Guopine, Hong Huacheng, Lu Ying, and Yue Shenling, ShanPhai Municipal Waterworks
Co . Shanfhei, China
In this paper, the efficiency of different water purification processes in
:emovinF organic muteger.s from raw water was introduced . We use initial "0"--
ozonation ; "T"-- trcditional rrocess ( comFiro+ion of coa!-ul .;tion,sedimentetion
c : .d filtration ) ; "C1"--chlorinQtion ; "F"-- bio_*iltratlon and "C"--bioactive
cerbon filtration . Thus, the 4 test systems (• combined processes) are 07C1,
fi9`Cl, FTCC1, and BTOCC1 .
Reeults showed that OT and FT processes were not al~le to chanre the mute'enic
nctivities of w~ter . One phenomenon a•e Pre interested in ie that the nurber
_' t^e revertents of the e:nters treated by OT and FT processes is approxime.•el;•
identical . However, after chlorination, the numFer of OTC1 effluent raised so
rapiely that it beceme more than thet of rew w .