Environmental and Molecular Mutagenesis - Legacy Tobacco ...

28 1989 EMS Abstracts
Notes electron microscopy, we determined that the detached elements were actually
aub-fragments of the unreplicated kinetochore . These fragments are short repeated
egments of 30 nm chromatin fibers which bind MTs and undergo the complete repertoire
of mitotic movements . Based upon these studies, a model for kinetochore structure
will be presented which explains new aspects of centromere structure, evolution and
involvement in aneuploidy .
http://legacy.library.ucsf.edu/tid/clb93d00/pdf
MOLECULAR ANALYSIS OF IN VIVO, SOMATIC MUTATIONS OF THE HPRT
GENE IN MOUSE AND MAN, Eleanor C . Brinson, Karolyn Burkhart-
Schultz, I M• l "Io, and Cheryl L . Strout, Biomedical Sciences
Division, Lawrence'.ivermore National Laboratory, Livermore, CA 94550 (USA)
To analyze factors that affect the spectrum of mutations in mammalian cells in
vivo, we are studying mutations of the hypoxanthine phosphoribosyltransferase
gene (hprt) in lymphocytes of both mouse and man . Gbmparison of mutations
detected in these systems with mutations detected in other in vivo and in vitro
systems will lead to better understanding of the effect that species, tissue, locus,
selection method, and sequence have on the spectra of both spontaneous and
mutagen induced mutations . Particularly in the mouse we are able to manipulate
factors that may affect the mutations recovered . in the mouse model, using
restriction fragment length analyses, we have detected a high proportion of
deletions among spontaneous mutations of the hprt gene . In nondeleted mutants
we have detected base substitution and frameshift mutations of the coding regions
by sequencing exon DNA, after amplification of genomic DNA using ahe
polymerase chain reaction (PCR). Studies of mutants isolated from irradiated mice
are in progress . Interspecies comparisons of spontaneous mutations are being
initiated with human lymphocytes by sequencing PCR amplified hprt cDNA. This
work performed under the auspicies of the U.S. Department of Energy by
Lawrence Livermore National Laboratory under contract number W-7405-ENG~48,
in part supported by an Interagenc A reement (Y01-ES-80171) between the
National Institute of Environmental Healt~ Sciences and DOE .
QICTOX .`C rlM BIOCMIICAL ACPIVITIES OF SCt'lE C3BACIMED ElHANES .
Brorizetti G ., I•Iorichetti E ., Del Carratore R., Rosellini D ., Paolini M., Cantelli Forti
G., Grilli S . and Vellosi R .
Istituto de A9utagenesi e Differenziamento, CtJR, Pisa
Istituto de Farmaoologia, Istituto di Canoerologia, Universita di Bologna, ITALY
1,1,1,2,-4+etrachloroethane (TIM), Pentachlaroethane (PCE) and Hexmchloroethane (F1CE)
have a wide range of applications in industrial processes as solvents, degreasers ard
aooeLerators in rubber vulcanization . 7he increasirg production of these caipoucrls in
the past few years led to major attention in un8erstanding their effects on huren health .
In this work, TiCT:, PCE and HCE were tested in D7 strain of yeast-Sacctwr -Cerevisiae
in suspension test with and withcut a mentnalian activation system S . TDCE, P(E~r Hf~
gave positive results on cells harvested fraa logarithmic