Environmental and Molecular Mutagenesis - Legacy Tobacco ...

158 1989 EMS Abstracts 457
Notes ~ -" IN VIVO IN
'I
TI~DS-TEST ON RAT HEPATOCYTFS EXPERIENCES IN TESTING XENOBiO-
http://legacy.library.ucsf.edu/tid/clb93d00/pdf
TICS AFTER SINGLE OR REPETITIVE TREATMENT .
E .C . Puri, Th . _krtnowd D . MOlier, Ciba-Geigy Limited ., CH-4002 Basle (Switzerland)
The in vivo-in vitro DNA-repair test gained significance during the last years and was also performed
in our laboratories for testing of chemical products as well as of the following reference compounds : 4aminobiphenyl
(ABP), benzo(a)pyrene (BaP), dimethylnitrosamine (DMN), methylmethanesulphonate
(MMS) and nafenopin (NAF). To investigate the effects of an enzymatic induetion of the liver a repetitive
treatment with two of these compounds was also carried out . Therefore, compounds were administered
either once (all five compounds), or repetitively (BaP, NAP). The preparation of hepatocytes
(perfusion technique) followed 2 hours after the single application of ABP (200 mg/kg), DMN (15 mg/kg)
or MMS (100 mg/kg) and 4 hours after the single application of BaP (100 mg/kg) and either 2 or 12
hours after the single application of NAF (200 mg/kg). For the repetitive treatment the animals was given
either BaP at a dose of 20 mg/kg or NAF at a dose of $00 ppm administered in the food on seven consecutive
days, followed at the eighth day by a dose of 100 mg/kg BaP 4 hours before isolation of hepatocytes
or by a dose of 200 mg/kg NAF either 2 or 12 hours before Isolation of hepatocytes . NAF did
not induce DNA-repair synthesis under any of the treatment conditions . Several experiments with ABP .
DMN and MMS yielded all clear positive results . BaP yielded a clear positive result after repetitive treatment
. These positive results obtained with BsP after enzymatic liver induction by the test substance itself
are noteworthy, inasmuch as earlier in vivo-in vitro experiments with the same substance on rat
hepatocytes (Mirsalis et al ., 1982, Environm . Mutagenesis 4, 553), rat pancreatic cells (Steinmetz and
Mirsalis, 1984, Environm. Mutagenesis 6, 321) and rat tracheal epithelial cells (Doolittle and Butterworth,
1984, Carcinogenesis S, 773), all performed without prior enzymatic induction, yielded negative results .
458
SOMiATIC NUTATIUN AND rtaCOMtlINA'TI0N TrST 0F FUnArYnINID0Nl ;, A Nr:W ANTIFILAaIAL AGEiVT,
IN Dti0S0YHILA hh:LANOGASTan
nei-Li Qian and A . F:iche
Shanghai Institute of Pharmaceutical Industry, 1320 Beijing Xi rtoad, Shanghai, China
Safety Assessment, Astra AS, S-15185, StidertRlje, Sweden
The somatic mutation and recombination wing spot test in Drosophila melanogaater
was used to evaluate the genotoxicity of Fluapyrimidone . Third inatar larvae, transhetero2ygous
for recessive wing trichome mutationa were f6II food/test compound mixturea
. A saturated solution or dilutions containing the test compound was added to
Instant medium (cxp 1) and powdered Furapyrimidone was added to Yeast-Torula (&xp 2)
at percentages of 0 .0j-S . F'urapyrimidone did not cause a significant change in the
mutant spot frequency for any ty ;,e of spots in l:xp 1 . In Fxp 2, the frequency of large
and twin spots was increased in flies treated with 0 .SdB Furapyrimidone . However
in repeated experiment the differences were statistically insignificant . Fwrapyrimidone
was positive on TA 100 and TA 98 at 0 .1 and 1 ntg/plate in Salmonella%microsome
system for mutagenicity test but teat on Sex-linked recessive lethal test in Drosophila
melanogaster Furapyrimidone showed no evidence of mutagenic potential .
Acknowledgmente : This work was done at Safety Assessment, Astra AB . We wish to thank
K . Sandelin and G . idexell for their technical assistance .
459
1SICROt1UCLEUS TEST IN VICIA PAnA ROOT TIPS : INITAOR1tICITT OP COAL TAR PITCH . Z . Qingfan,
ChangFuju, and Z . Qingxia . Dept . of Pathology, Henan Medical University . Zhengzhou,
Henan, P .R . China .
The effect of three different processed (high, moderate, low temperature) coal tar
pitches on the induction of micronuclei in yjglg ZAbg root tips was studied . The results
indicated that CTP had potent mutagenicity on'the root tips of y1gjA I&a, but the
mutagenicity may vary in different kinds of CTP .
TABLE . Frequencies of Micronucleated cells in }(jsL j4g root tips induced by CTP
1oa1 ~ T,
ar Pitch Croilp - maan3SD (a)
Control Grouo
Concentration (mg/ml) j, OZa =s
0 .324 14 .33t0 .88 36 .33±4 .44 16 .33t0 .88
(16 13 14) (38 43 38) (16 18 15)
6 .828 39 .67t1 .76 59 .00±3 .46 26 .33t2 .91 1 .67±0 .67
(39 43 37) (53 65 59) (27 21 31) ( 3 1 1)
10 .0 60 .33±8 .08 82 .00t9 .61 60 .67±6 .49
(49 76 56) (63 94 89) (58 73 51)
These results are consistent with the study on rat lung cancera induced by
intratracheal instillations of CTP, which were made by us previoualy, Through the
experiment, we consider that the micronucleus test in yicig ZAg can be used as a new
alarm system to detect envirorueental pollution such as CTP, and that this test has the
advantages of sensitivity, reliability, low cost and manageability .
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