Environmental and Molecular Mutagenesis - Legacy Tobacco ...
Environmental and Molecular Mutagenesis - Legacy Tobacco ...
Environmental and Molecular Mutagenesis - Legacy Tobacco ...
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214 1989 EMS Abstracts<br />
---~- .~-- _-<br />
Notes-<br />
of' mutation in ~e [f, the different mutation induction methods used in the experiments<br />
can be arranged in the following sequence : treating zygotes at late stage > treating<br />
zygotes at early a~a~treating dry seeds . The results indicated also that the zygotes<br />
receiving the BL4 post-treatment seem to be equipped with an imperfect DNA-repair system<br />
of enzymes because their mutation ratio is not obviously higher than that of the variant<br />
zygotes not receiving Bt2t post-treatment .<br />
http://legacy.library.ucsf.edu/tid/clb93d00/pdf<br />
- 621--<br />
A cTUDY OF SCE IivDUC'PION BY TRIPTi•,'RYGIUM HYPOGLAUCUM(LEVM . ) HUTCH IN<br />
HUMAN PERIPHERAL BLOOD LYMPHOCYTES<br />
Wang Xu, Zhou Rumin <strong>and</strong> Ceo Neng . Department of Biology, Yunnan<br />
Normal University, Kunming, Yunnan, Peo le's Republic of China .<br />
Tripterygium hypoglaucum (Level .) Hutch (pTHH) is a fChinese tr<strong>and</strong>itional<br />
medicine which has been used for the treatment of various<br />
diseases such as systemic lupus erythematosus, rheumatoid arthritis etc .<br />
The medicine showed antifertility effects both in rat <strong>and</strong> human during<br />
the medicine taken period . No other cytotoxic effects were reported .<br />
Our work aimed at the cytogenetic research of THH . The presented<br />
work emploged the sister chromatid exchanges (SCEs) to assess its<br />
genotoxic effects in human peripheral blood lymphocytes .<br />
Blood for all the experiments were obtained from both healthy donors<br />
<strong>and</strong> patients cured with TH .9 for over 90 days <strong>and</strong> treated by THH with<br />
the doses from 200 to 0 .63mg/ml in vitro . The blood from every donor<br />
were cultured in two ways of presencing 69 mix or not .<br />
The results indicated THH didn't induce SCE siRgnificantly in human<br />
lymphocytes from healthy donors <strong>and</strong> patients cured with THH before<br />
sampling . S9 had no effects on SCE induction by THH in all the cases .<br />
However, THH showed a strong cell cycle delay effects when higher<br />
treating doses from 200 to 10mg/ml were used in vitro .<br />
Our results suggest that THH could be a human anti-baby medicine .<br />
Further research about its other cytogenetic <strong>and</strong> cytotoxic effects are<br />
being done in our lab .<br />
622<br />
STUDIES ON THE NUTAGENTC . CARCINOGFNIC AND TI]tATOGDU C EFFSCTS OF l,2-UD101 AND ETEPA<br />
Wang Zhi-qlao, Li Xiu-yin', tlen Si-zhes, Liao Nia'-yaaq<br />
The studies of mutayeaic effects of 1,E-UD101 <strong>and</strong> setepa were investi'ated by Anes test,the bone<br />
narrow micronucleus test of sice,saalysis of bone marrow chranosome aberration of rats,analysls of<br />
chromosome aberration <strong>and</strong> SCE test In bn .aa peripheral blood lymphocytes, analysis of chromosone<br />
aberration <strong>and</strong> SCE of sice spersatoyenous cetls, the do .inant lethal test of sice . The results<br />
showed 1,=-UDIDf was re0ative <strong>and</strong> metepa was positive In all test systens . Nice I drinkiny water<br />
freely for one year which contained 1,2-I1DIIX at the coaceatratlon of 30-300my/1 highly Induced<br />
beaangiaatous lesions of liver <strong>and</strong> adeaotous changes of Lung . The lumoriyenicity of inetepa did<br />
not be induced at the concentration of 1-10n9i1 during experinrat of one year .The teratoyenic<br />
test of rats showed setepa maialy Induced limb ∎alfornation whea It was i .p . ad.laistered at<br />
the dosage of 30sy/kg.Tle malformation frequency was 9f% .aad 1 .=-UDlDI had so teratoyenic effects .<br />
The further study with rabbits showed metepa had so terato'enic effects . The results of the<br />
study Indicated that 1,2-UDIOi ∎ibd belong to epioenetic carcinogen <strong>and</strong> netepa might belong<br />
to carcinogen with mutaoenesis .<br />
623<br />
INDUCTION OF THIOGUANINE RESISTANT MOUSE LYMPHOCYTE VARIANTS BY SUB-<br />
CHRONIC LoW DOSE INHALATION EXPOSURE TO BENZENE . J . B. Ward Jr ., M . M .<br />
Ammenheuser, D . L . Morris, V . M . S . Ramanujam, <strong>and</strong> M . S . Legator Dept .<br />
of Preventive Medicine <strong>and</strong> Community Health University of Texas Medical<br />
Branch, Galveston, TX 77550, USA .<br />
Spleen lymphocytes of CD-1 male <strong>and</strong> female mice exposed to benzene<br />
vapor were evaluated for the frequency of thioguanine resistant variants<br />
(Vf) . The nice were exposed, by inhalation, 22 hours per day for six<br />
weeks to benzene concentrations of zero (air only), 40, 100, <strong>and</strong> 1,000<br />
parts per billion (ppb) or were housed in st<strong>and</strong>ard cages (cage<br />
controls) . An autoradiographic assay for Vf was used with cryopreserved<br />
cells from three animals of each sex at each exposure . The mean Vts'were<br />
zero, 7 .04 ; 40 ppb, 26 .25 ; 100 ppb, 52 .10 ; 1,000 ppb, 20 .39 <strong>and</strong> cage<br />
controls 13 .25 . Vts in females were consistently higher than in males in<br />
benzene exposed animals but not in controls . Confidence intervals (95%)<br />
50869 3728
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