Environmental and Molecular Mutagenesis - Legacy Tobacco ...
Environmental and Molecular Mutagenesis - Legacy Tobacco ...
Environmental and Molecular Mutagenesis - Legacy Tobacco ...
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688<br />
CYfOGENETIC EFFECTS OF PHORBOL ESTER TUMOR PROMOTERS : POSSIBLE ROLE IN IIULTISTEP TUKORI-<br />
GENESIS. V . Kinzel . B. Farber, R .T. Petrusevska . N . E . Fusenle . Institute of Exp. Pathology,<br />
Institute of Biochemistry', German Cancer Research Center . D-8900 Heldelber¢ . PRG<br />
Tumoriyenesis in mouse skin can be effected in a number of steps : by initiation with DMBA, by<br />
conversion with one or two applications of TPA (12-0-tetradecanoylphorbol-13-acetate) <strong>and</strong> by reprated<br />
treatment with RPA (12-0-retinoylphorbol-13-acetate) . The conversion step effected by TPA<br />
(but not by RPA) Is characterized by a half-life of 10-12 weeks (in NMRI aice) : it is Sndependent<br />
from initiation (Fiirstenberger . G . et al ., 1985, Science, L3-0. 76) . '1'his specific effect of TPA<br />
may be explained best by the clastogenic activity of TPA shown In mouse keratinocytes In culture<br />
(Petrusevska, R.T. at al . 198fi, Carcirto¢enesis $, 1207) as well as in ex vivo studies (Fiirstenbereer,<br />
G . et al ., 1988, Carcino¢eneals . In press). Details of the olastoYenic action of TPA were studied in<br />
HeLa cells which exhibit a rodiuaimetic cell cycle response to TPA (Klnzel, V . at al ., 1980, Science<br />
210 . 429) . Only TPA exerted a significant elastogenic activity at non-eytotoxic concentrations (10-8<br />
to 10-6 M) measured after 24 <strong>and</strong> 48 hrs . Values obtained with RPA were close to those obtained in<br />
the presence of solvent (acetone 0.2k). The response to TPA was only to some extent correlated with<br />
the dose. Chromosome aberrations including gaps <strong>and</strong> breaks were predominantly of the chromatid type ;<br />
they are earliest measurable after 6-8 hrs, I .e. as the cells recover from TPA-induced 02-inhibition<br />
(Kinzel . V . et al., 1988. Cancer Res . 40 . 1759). A 30 ∎in exposure to TPA (10-7 N) is sufficient to<br />
induce aberrations . The data point to an indirect, possibly receptor-mediated action of TPA . If it<br />
is supposed that TPA-iuduced chromosome lesions represent a key event required for conversion it<br />
might be further speculated that the absolute requirement for DNA replication In the conversion step<br />
(Kinsel, V . et a1 ., 1984 . PNAS $1. 5858) is necessary to "fix" a certain degree of chromosome damaQe<br />
(supported by the DFG) .<br />
689<br />
ASSESSMENT OF EXPOSURE AND POTENTIAL RISK FROM MUTAGENIC AIR POLLUTANTS . Joellen<br />
Levtas, U .S . <strong>Environmental</strong> Protection Agency, Research Triangle Park, N .C . 27711<br />
(U .S .A .) .<br />
Genetic bioassays have been used to identify airborne mutagens <strong>and</strong> tlieir sources,<br />
to measure human exposure <strong>and</strong> to provide comparative assessment of potential cancer<br />
risk from air pollutants . Recent air pollution studies have integrated the use of<br />
genetic bioassays into sampling <strong>and</strong> analysis strategies to seet these goals . Complex<br />
mixtures of urban air pollutants from vehicles <strong>and</strong>aresidential heating sources have<br />
been studied in field investigations in both Western <strong>and</strong> Eastern cities in the U .S .<br />
as part of EPA's Integrated Air Cancer Project . Genetic bioassays were applied to<br />
source emissions <strong>and</strong> ambient outdoor <strong>and</strong> indoor air to characterize the exposure <strong>and</strong><br />
potential risk from the gaseous, semi-volatile <strong>and</strong> particle-bound organic species .<br />
Source apportionment of the mutagens in these airsheds has been accomplished through<br />
receptor-modeling of mutagens using multiple linear regression analysis .<br />
Mutagenesia, tumorigenesis <strong>and</strong> DNA adduct dosimetry studies of these air pollution<br />
mixtures have been used in further developing a comparative potency method for<br />
assessing cancer risk from complex mixtures . This is an abstract of a proposed<br />
presentation <strong>and</strong> does not necessarily reflect EPA policy .<br />
690<br />
THE GENETIC TOXICITY OF THE HUMAN CARCINOGENS BENZIDINE AND BENZIDINE-<br />
BASED DYES : CHROMOSOMAL ANALYSIS IN EXPOSED WORKERS<br />
E .Mirkova <strong>and</strong> S .Lalchev,The Medical Academy,Sofia (Bulgaria)<br />
The activities of the human carcinogens benzidine (BENZ) <strong>and</strong> BENZ-based<br />
dye Direct Black 38 in the rodent bone marrow micronucleus assays ( BM<br />
MNA) were established unequivocally (Ashby <strong>and</strong> Mirkova,1988 ;Beije 1987) .<br />
The lack of data on their genetic effects in humans acted as the stimulus<br />
for the present cytogenetic study .Chromosomal analysis was performed<br />
in the lymphocytes of 23 BENZ-based dyes manufacturing workers <strong>and</strong> 30<br />
matched control .individuals . The period of exposure was 7-31 yr . BENZ<br />
was detecSed in the workplace air at concentration levels of 0 .42 -<br />
0 .86 mg/m <strong>and</strong> in the urine of exposed workers respectively at mean level<br />
of 1 .78 ± 1 .4 µg/1 . The total airborne particulate levels of BENZ -<br />
based dyes (mainly Direct Black 38) ranged from 7 .8 to 32 .3 mg/m3 . i<br />
significant increase in the X of aberrant cells (-gaps) was found in the<br />
exposed group (7 .9 ± 5 .9) as compared with the control incidence of<br />
0 .87 ± 0 .26X aberrant cells (-gaps) . Aberrations were mainly chromatid<br />
breaks . The X frequency of polyploid cells in the exposed grou was<br />
found to be 1 .1 ± 0 .1 <strong>and</strong> the control incidence was 0 .17 * 0 .5~ . The<br />
present data "establish that the rodent BM MNA has predicted correctly<br />
the mutagenicity to humans of the IARC carcinogens BENZ <strong>and</strong> BENZ-based<br />
dyes .<br />
http://legacy.library.ucsf.edu/tid/clb93d00/pdf<br />
1989 EMS Abstracts 237<br />
Notes