Environmental and Molecular Mutagenesis - Legacy Tobacco ...

220 1989 EMS Abstracts, . ~ --
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http://legacy.library.ucsf.edu/tid/clb93d00/pdf
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NOtES gene transfer recipients for a normal human fibroblast cDNA library prepared in the
pc02 expression y~ect~ Using a low serum/BUDR/nUV light selection, several Independent
cDNA-induce$BM revertants have been isolated from cultures transfected
with the pcD2 cDNA library that were greater than 1000-fold suppressed for anchorage
independence relative to the parental BHKA tumor cells . Progress in characterization
of these cONAs associated with tumor suppressor gene function will be presented .
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SEIECPION OF VF111CL.E MD THE AOfRE OF M14MSTRATICt!1 IN T1B; MD(JSE BfltE MARRQW
MICRONUCLEUS TEST . J .P . Wojciechowski+, D .K . Gulatil, and M D . Shelb jy
lEnvironmental Health Research and Testing, Inc ., Lexington, KY ~itre National
Institute of Environnental Health Sciences, Research Triangle Park, NC .
Two critical factors that influenoe the swrimum exposure of the target tissue
with the test material in the bone marrow micranucleus test are the route of
administration and the vehicle . The purpose of this study was to determine the
extent to which vehicle and route of administration affect level of effect .
Dimethylbenza(a)anthraoene (DNIDR), N,N'-methylenebisacrylamide (MR),
Nynethylolacrylamide (NOA), and 4-aminobiphenyl (ABP) were tested using a variety of
vehicles and two different routes of ad+ninistration . DlIBA suspended in corn oil and
administered via lP at 10 ug/kg caused a 2 .9 told increase in the incidence of
micronucleated polychronatic erythrocytes (Md-FCE) over the vehicle control . D)a3A
dissolved in Dr9O (lOmq/kg) via lF resulted in an 11 .3 fold increase in t4}-FCE . l81R
in DMSO via lF had no effect on Mi-FM (p>0 .1) at up to SOsiq/kg . MBA dissolved in
PBS (50mg/kg) caused a 16 fold increase in FQ}-PCE's . 143ii dissolved/suspended in
PBS, corn oil, or D6s90 and administered via lP at 112 .5 sq/kg gave a negative
response for M-FCE. MON in PBS via gavage caused a weak but significant increase
(p