Environmental and Molecular Mutagenesis - Legacy Tobacco ...

230 1989 EMS Abstracts
TFIE EFFDLT OF DIETAFB! B1diSSIMS SPAWiS CN 7~ GENOTWQCTIY OF AFLi~T03mi, Notes
D AND 2-ACLZYLAIIDFiDl7RFIJE . C .M . Hatniltcnt, R .M . Iblmest . ~ P .
Bakke~, K .E . Garinf , K.L. Steirmetz', G .S . Steawsand2, and J .C . llirsalist . SRI
International, Menlo Park, CA, and 2Corne11 University, Geneva, NY .
Dietary oortstmgtion of civcifervus vegetables, especially lmmsels sprouts, have
been shown to decrease hepatocarcinogerticity of aflatAxin 13~ (AFBi) in rats, but the
medhanisn of this effect is tatlvfam . We e~mined the ef ect of di bzussels
sprouts (~ Q, L . ) on tha gertotwdci of senrezal krtv.m carcirogens in
male Fisdaer-344 zats . Rats wer+a fed a basal diet~80) or a brussels sprouts amerded
diet (SSAD ; 20% freeze-dried btussels ) for apQrwtimately 3 weeks prior to
testinq . Ur~3iedu .led ON71 is was aieesured in ilepatocytes expesed to
AFSi, dimethylnitrosamine ~j~ ~-acetylaminofhsoresfe (2-AA ) . BD rat
exposed to 0 .0005, 0 .001, and 0 .005 µq/ml yielded -0.9, 6 .2, and 22 .0
~g~rau~is/twcleus (NG) . respectively, with 24, 54, and 92% of eells in repair (tIIt,
t