Environmental and Molecular Mutagenesis - Legacy Tobacco ...

86 1989 EMS Abstracts
http://legacy.library.ucsf.edu/tid/clb93d00/pdf
Notes treated with blue cotton . Elution of the cotton with organic solvents, most efficiently
with ammoniacal methanol, results in recovery of the adsorbed polycyclics . With this
method, a high concentration extent is generally obtainable, and the entire work needs
only short periods of time and little labor . Since mutagenic heterocyclic amines and
polycyclic aromatic hydrocarbons are efficiently adsorbable to blue cotton, the use of
this method allows monitoring of these classes of mutagens in food . It has been shown
that many proteinaceous foods that had undergone heating processes contain mutagens . in
most cases the heterocyclic aminee ; e .g ., katsuobushi, a heat-dried bonito-fish meat,
which is one of the everyday food items in Japan, has been shown to contain MeIQx . We
have also observed that some shell fish in their raw state contain mutagens . We have
recently improved the method by introducing blue rayon, an adsorbent more powerful than
blue cotton . The blue-cotton and -rayon techniques can also be used for isolating foodmutagen
metabolites in excretions and body fluids, and for monitoring mutagens in
environmental waters and airs . (Supported by grants from the Ministry of Health and
Welfare, Japan)
245
GENOTOXICITY OF INITIATING CARCINOGENS IN THE SKIN .
S . He and R .S .U . Baker, National Institute of Occupational Health & Safety, Building
A27, University of Sydney . NSW 2006 (Australia) .
Nicronucleus(MN) induction can be evaluated in keratlnocvtes followinR application
of chemicals onto the dorsal skin surface of hairless HRA/Skh mice . Previous studies
have indicated that animals of this strain are sensitive to chemical induction of
akin cancer, and also that the active alkylating carcinogen, triethylenemelamine, can
be readily detected as a genotoxicant in keratinocytes using this procedure . For
chemicals requiring in vivo metabolism, it was first necessary to establish an
appropriate sampling time between chemical treatment and removal of skin for in vitro
studies . With 7,12-dimethylbenz[a]anthracene (DNBA), MN induction reached maximal
values at 12, 24 and 48h post-treatment . A sampling time of 24h was therefore
selected for all chemicals . Dose-related increases in HN were detected with the
initiating carcinogens DMBA, benzo[a]pyrane (BP), chrysene and urethane while pyrene
failed to induce MN at subtoxic doses up to 2 .5mg/mouse . Significant MN induction
(p