Environmental and Molecular Mutagenesis - Legacy Tobacco ...

180 1989 EMS Abstracts e .
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http://legacy.library.ucsf.edu/tid/clb93d00/pdf
NOteS strains TA 9'BDNP (arylhydroxylamine esterase deficient) or TA 98NR/DNP
(nitroreductasel,, and arylhydroxylamine esterase deficient) . 1,3-
Dinitropyren8---EFRF-1,6-dinitropyrene wer weakly mutagenic to TA 98 at
doses similar to 1,8-DNP . Studies with [14C] 1,8-DNP showed that 1 hour
after oral dosing (lmg/kg), over 100nq of DNP were present in the liver
(w 0 .73% dose) . However, only about 5 .5ng were present in the bacterial
pellet, suggesting that hepatic components, in vivo as in vitro, bind
to DNP, thus preventing its interaction with Salmonella .
522
1,8-DINITROPYRENE : DISTRIBUTION AND EXCRETION IN THE MOUSE .
A .B .Shah, I .R.Rowland and R .D .Combes, School of Biological Sciences,
Portsmouth Polytechnic, U .K ., B .I .B .R .A ., U .K ., and I .R .I . Ltd ., U .K .
Despite considerable interest in the toxicology of 1,8-dinitropyrene
(DNP), little is known of the toxicokinetics of this mutagen . We have,
therefore, investigated the distribution nd excretion of DNP following
oral administration of a single dose of C-DNP (0 .25mg/kg) to female
BALB/c mice . At known times (2,4,6,24,48,72,96,168, and 216 hours)
after dosing, mice were killed, various tissues removed and their
radioactivity determined. The results indicate a slow uptake of DNP
into the bloodstream and other tissues . Maximum amounts were present in
the bloodstream, liver and kidneys after 6 hours, representing 0 .274,
2 .9% and 0 .21% of the radioactive dose . The radioactivity in these
tissues decreased after 24 hours to 0 .1%, 1 .6% and 0 .12% respectively,
after which there was a gradual decrease amongst these and all the
other tissues studied . Most of the excretion was within 48 hours of
dosing. At 72 hours, radioactivity recovered from all the tissues
studied and urine and faeces amounted to