February 15-18, 2009 Washington State Convention Center Seattle ...

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INVESTIGATION OF SPECIES SUSCEPTIBILITY TO VHVS TYPE IVB AND
INVESTIGATION OF THE USE OF IODINE EGG-DISINFECTION ON VHSV
Geoffrey H. Groocock*, Stephen A. Frattini, Rodman G. Getchell, Gregory A. Wooster, William T. Heath,
Kristine M. Hope, Rufina N. Casey, Steven R Lapan, Andrew D. Noyes, James W. Casey and Paul R. Bowser
Aquatic Animal Health Program
Department of Microbiology and Immunology
College of Veterinary Medicine
Cornell University
Ithaca, New York 14853 USA
The effect of VHSV in the Great Lakes may have a significant potential impact on aquaculture and recreational fishing species
such as channel catfish, walleye and muskellunge. Currently there is little information known on the relative susceptibility
of VHSV type IVb on these different species. Infection trials were performed on each of these species. Fish were inoculated
using the type Michigan 2003 isolate by immersion in water containing a known concentration of virus for one hour or by direct
intracoelomic injection of a small volume of known virus concentration. Control fish were sham inoculated using a sterile
sample of the media used to propagate the virus. Initial results of these experiments will be discussed.
Additionally, the effects of topical egg disinfectants such as iodine on VHSV are unknown. A trial experiment to determine
whether VHVS can be detected in eggs after exposure to VHSV containing water was performed. The experiment included
varying doses of VHVS, different concentrations of iodine disinfection and different methods of reducing egg adhesiveness
using tannic acid or Fuller’s earth. The result of the trial experiment will be presented and the proposed full experimental trial
will be discussed.
BIOACCUMULATIVE CONTAMINANTS IN PADDLEFISH IN LARGE RIVER SYSTEMS OF
THE UNITED STATES
Deke Gundersen and Steven D. Mims
Environmental Science Program
Pacific University
Forest Grove, OR 97116 USA
deke@pacificu.edu
Paddlefish inhabiting impounded large river systems that are influenced by industrial and agricultural processes are susceptible
to bioaccumulation of persistent bioaccumulative contaminants. Contaminants that are a particular problem in many of these
large river systems throughout the United States include chlorinated hydrocarbons (i.e PCBs, DDT, chlordane, and dioxins) and
methyl mercury. Many studies indicate that these contaminants negatively impact the health of paddlefish populations inhabiting
these large river systems. In addition, the chemical properties of these contaminants allow them to bioaccumulate in the
edible tissues of these species. This can potentially pose health risks for people consuming edible products from these species
(roe and meat), particular certain susceptible individuals (pregnant women and young children). This paper will review previous
studies investigating bioaccumulative contaminants in paddlefish in large river systems of the United States and compare
to farm-raised paddlefish as well as the conditions in these large rivers that make these long-lived species susceptible to uptake
of these chemicals.