February 15-18, 2009 Washington State Convention Center Seattle ...

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THE INFLUENCE OF LYSINE DEFICIENCY ON YELLOW PERCH (Perca flavescens)
FEMALES REPRODUCTIVE PERFORMANCE AND THE QUALITY OF EGGS
Karolina Kwasek *, Konrad Dabrowski, Joanna Nynca, John M. Reddish and Macdonald Wick
The Ohio State University
Department of Animal Sciences
2029 Fyffe Court
Columbus, Ohio, 43210 USA
kwasek.1@osu.edu
We have previously shown that yellow perch fed wheat gluten protein-based diets supplemented with free lysine, (+) Lys diet,
achieved significantly larger mean weights than yellow perch fed a diet deficient in lysine (- Lys) (Kwasek. et al. 2008). We
have also shown that lysine deficiency has an impact on overall gametogenesis and frequency of body deformities of yellow
perch broodstock, and influences the maturation and sperm quality in this species. The objectives of the present study were:
(1) to determine whether Lys influences the ovulation of yellow perch, and (2) to evaluate whether Lys has an impact on free
amino acid pool of yellow perch eggs.
Yellow perch juveniles raised on formulated commercial diets at an initial size of 12.9 ± 4 g were individually marked with
pit-tags and randomly distributed into six 400 L tanks, 45 fish per tank. This experiment included two diets: (-) Lys-deficient
and (+) Lys-supplemented (2.23 % dry basis) wheat gluten-based diets in triplicates. Control females were fed a commercial
diet. Females from control, +Lys and –Lys groups that had ovulated were stripped and their eggs divided into 0.4-1.2 g (0.77 ±
0.11 g) portions and mixed with sperm (21.4 ± 4.3 μl). Samples of eggs were weighed, counted and stored.
Our results showed that the mean weight of females fed experimental diets and their relative egg weight ratio (Fig.1.) were
not significantly different. Females from +Lys group had significantly larger condition factor than those from –Lys group. The
largest amount of eggs was obtained from control fish in comparison to fish from the other treatment groups. The mean survival
was higher in eggs from control and +Lys females (15.2, 13.8 %, respectively) in comparison to –Lys females (5.13 %). The
free amino acid levels in eggs remain to be evaluated.