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February 15-18, 2009 Washington State Convention Center Seattle ...

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ON CHOICE AND USE OF STATISTICAL TOOLS IN EXPERIMENTAL FISH NUTRITION<br />

STUDIES<br />

Juhani Kettunen and Kari Ruohonen<br />

Finnish Game and Fisheries Research Institute<br />

Viikinkaari 4<br />

FIN-00791 Helsinki<br />

Finland<br />

juhani.kettunen@rktl.fi<br />

The main aim of the paper is to discuss and demonstrate the sequence of decisions that are necessary when using statistical<br />

models in fish nutrition studies. We emphasize the key role of statistical experimental design that is, unfortunately, too often<br />

flawed or completely missing in present studies. Furthermore, we demonstrate the choice of model that is based on the scale<br />

and distribution of response and predictor variables. We recommend an iterative experimental strategy. Thus, instead of a single<br />

one-shot experiment we prefer a set of consecutive, complementary experiments that are designed on the basis of existing<br />

knowledge and outcome of the preceding trials. The philosophy behind is to start from screening the relevant variables with<br />

simple and cost-effective designs and proceed towards more detailed understanding of the system with more targeted and often<br />

more complicated designs. We speak of model-based design and analysis of fish nutrition problems. By doing this we design<br />

our experiments to minimize the posterior uncertainty of the model parameters and forecasts. For this the choice of a model on<br />

the basis of the scale and distribution of response and predictor variables is crucial. In the paper, we compare the model-based<br />

approach with the traditional design-based approach that emphasizes statistical hypothesis testing in finding significance of effects.<br />

We demonstrate many strengths of model-based approach in comparison with design-based one. Most important strength<br />

of the model-based strategy is the information that is given to decision makers, i.e. users of the results. While the design based<br />

approach gives him/her a on-off solution, the model-based leaves the final decision to the decision maker. This is possible,<br />

because also estimates of the risk and uncertainty levels are produced.<br />

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