February 15-18, 2009 Washington State Convention Center Seattle ...

PRELIMINARY STUDY OF THE EFFECTS OF ELEVATED DIETARY CADMIUM ON
GROWTH AND CADMIUM ACCUMULATION IN TISSUES OF JUVENILE PARROT FISH,
Oplegnathus fasciatus
Okorie E. Okorie, Jun-Ho Lee, Seunghyung Lee, Young Chul Kim, Jun-Young Bae, Gun Huyn Park
and Sungchul C. Bai*
Department of Aquaculture/Feeds and Foods Nutrition Research Center
Pukyong National University
Busan 608-737, Republic of Korea
scbai@pknu.ac.kr
An 8-week feeding trial is going on to evaluate the effects of elevated dietary cadmium on growth and cadmium accumulation
in tissues of juvenile parrot fish, Oplegnathus fasciatus, averaging 5.5±0.06 g (mean±SD). Seven semi-purified experimental
diets were formulated to contain 0, 100, 200, 400, 800, 1600 and 3200 mg Cd/kg diet in the form of cadmium chloride (CdCl 2 ).
Casein and white fish meal served as the protein sources, wheat flour, dextrin, and corn starch as the carbohydrate sources, and
fish oil as the lipid source of the experimental diets, which were isonitrogenous and isoenergetic, and contained 50% crude
protein and 17 kJ available energy/gram diet. After two weeks of acclimation, fifteen parrot fish were randomly distributed into
each of 21 acryl tanks of capacity 30 liters. Each diet is being fed to triplicate groups of fish twice a day (1000 and 1600 h) to
apparent satiation (approximately 4% of wet body weight per day at the beginning and planned to be reduced to 3% of wet body
weight per day at the end of the feeding trial). At the end of 8 weeks of feeding trial, weight gain (WG), specific growth rate
(SGR), feed efficiency (FE), protein efficiency ratio (PER), and survival will be measured. Hematological characteristics to be
determined include hematocrit (packed cell volume – PCV) and hemoglobin, Hb (g/dl). Cadmium accumulation in gill, liver,
muscle and kidney will be determined.
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