February 15-18, 2009 Washington State Convention Center Seattle ...

PRODUCTION OF Artemia CYSTS AND FLAKES USING IMPROVED TECHNIQUES IN
ROCK SALT SOLUTION
San Ei Ei Maung*, Dr. Hnin Hnin Htun and Dr.Mar Too Nyi Bu
Aquaculture Biotechnology Research Center
Department of Biotechnology
Mandalay Technological University
Myanmar
The high quality (disinfected and good nutrient level) Artemia cysts could be firstly produced in our country using rock salt
solution without adding other chemicals. Initially, the seven Artemia cysts brands were hatched to know the best and suitable
brand. Secondly, many ways of disinfection and decapsulation procedures were tested depending on hatching efficiency.
Thirdly, Artemia were cultured depending on the effects of variable feeds (energy and protein source). For this, there were
three experiments, the first one was the use of rice bran suspension, the second was the use of yeast + vitamins suspension,
and the third was the use of rice bran + yeast + vitamins suspension. After one month, it was found that the third was the fastest
metamorphosis and the highest cysts production. Fourthly, the disease challenge tests were made using bioencapsulation
method (boosting). For this, there were four experiments, the first one was natural (control), the second was the use of neem
leaf decoction, the third was the use of probiotics, the forth was the use of antibiotics. After one month, the lowest bacterial
count and highest survival rate were showed by the second and third. Finally, by using the best data from the above experiments,
high quality Artemia cysts were produced. The hatching efficiency of this produced cysts were tested two times per
month and showed the best data. As the by-products from cysts production, Artemia flakes (protein 53%) were produced for
post larvae. So, this Artemia cysts and flakes production technology will be suitable for the places where cannot find naturally
Artemia cysts and cannot use natural seawater to produce marine aquatic feed products (Artemia cysts and flakes) and will effective
not only aqua-farmers but also salt-farmers by combination with solar salt and Artemia cysts production technology to
get extra-income.
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