February 15-18, 2009 Washington State Convention Center Seattle ...

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STUDING THE ULTRA STRUCURAL IN LIVER AND KIDNY BY USING ELECTRON
MICROSCOPY AS WELL AS HEMATOLOGICAL PICTURE AND MEASURING
BLOOD PARAMETER DUE TO EXPERIMENTAK INFECTION WITH FLEXIBACTER
COLUMNARIS IN MONOSEX TILABIA
Dr. Adel Younes
Department of Veterinary Services
Egypt
A total number 25 apparently health Monosex tilapia fish average body weigh 70 gm . five fish were randomly sampled and
submitted to bacterial isolation for verification of absence of tested bacteria .The other 20 fish divided into two group 10fish
for each group first 10 fish were injected intramuscularly with 1 ml of virulent strain of flexibacter columnaris adjusted at
McFarland No.2 (approximality 6x10
.
-8 cell /ml . The second group10 fish were injected with 1 ml sterile saline and served
as control. The site of injection was dorsal muscle just behind the head .Aeration was maintained and temperature ranged from
25 – 27 C according to Baxa et al (1987).The clinical signs and PM lesions were observed during the experiment .Specimens
from liver kidney spleen and gills were collected and use for histopathological studies .Moreover blood samples were collected
for determined hematological picture and blood parameters .Sample from liver kidney were taken at zero time from control
group and at 12.24,48,72and 96 hours for ultra structural three infected fish were used in case of each sample. The period of
this experiment was 96 hours
Fig. (1) Differential leucocytic Fig. (2) Serum protein constituent Fig. (3): Serum enzyme level
Fig. (4). Liver of Monosex tilapia 72 hours Fig (5). Kidney of Monosex tilapia 12 hours post
post injection-showing apoptosis(x 4000). injection-showing Mitochondrial swelling (M),
formation of myelin fibers (↑), loss of cytoplasmic
matrix and rough endoplasmic reticulm (RER)
, (x10000)