February 15-18, 2009 Washington State Convention Center Seattle ...

GROWTH OF PACIFIC WHITE SHRIMP Litopenaeus vannamei FED DIETS CONTAINING
DIFFERENT LEVELS OF SOY PROTEIN CONCENTRATE REARED UNDER CLEAR
WATER SYSTEM
Daranee Sookying*, Patricio Paz and D. Allen Davis
Department of Fisheries and Allied Aquacultures
Auburn University
Auburn, AL 36849-5419 USA
sookyda@auburn.edu
The study on the use of soy protein products as alternative protein sources in shrimp diets has been a focus all over the world
because of their favorable protein content, suitable amino acid profile, economic opportunity, and consistent quality. Previous
research demonstrated the potential of using soy protein concentrate (SPC) at low level as replacement of solvent extracted
soy bean meal (SE-SBM) in shrimp feed. However, as high levels resulted in reduced growth further work was warranted. The
objective of this study was to confirm the growth response of Pacific white shrimp Litopenaeus vannamei fed practical diets
containing different levels of soy protein concentrate in substitution of soybean meal, when reared in the clear water condition.
Two trials were carried out at the E.W. Shell Fisheries Research Station (EWS) in Auburn, Alabama and Claude Peteet Mariculture
Center (CPMC) in Gulf Shores, Alabama.
In the first trial, six dietary treatments containing increasing percentages of SPC (0%, 5%, 10%, 20%, 40% and 40% with
additional fish soluble) were randomly assigned amongst 24 tanks (80 L) with four replications per treatment. Juvenile shrimps
(initial weight 0.12 g) were stocked at a density of 12 shrimps per tank during an 8 week feeding trial. Daily feed inputs were
calculated, based upon an expected weight gain of 0.8 g per week and an expected feed conversion ratio of 1.8:1.
In the second trial, four dietary treatments were produced under commercial conditions and the diets were randomly assigned
amongst 12 tanks (425L) with three replications per treatment. The experimental diets contained increasing level of SPC (0%,
4%, 8%, and 12%). Juvenile shrimp (0.9g initial weight) were stocked into the tanks at a density of 15 shrimp per tank during a
12 week feeding trial. Daily feed inputs were calculated, based upon an expected weight gain of 1.0 g per week and an expected
feed conversion ratio of 1.5:1.
Water exchanged was done at a rate of 100% every day with a re-circulating system containing a biological filter, and a circulation
pump throughout the trial period. Dissolved oxygen, temperature, and pH were measured twice a day, and total ammonianitrogen
was measured biweekly. At the end of the experiment, growth, survival and feed conversion ratio from both systems
will be determined and compared to examine the variation performance of Pacific white shrimp.
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