February 15-18, 2009 Washington State Convention Center Seattle ...

FORMULATION AND APPLICATON OF PROBIOTIC AS BIOCONTROL AND BIOSAFETY
FOR ORGANIC AQUACULTURE SYSTEM
Dr. Ohnmar Aung* and Dr. San San Yu
Aquaculture Biotechnology Research Center
Department of Biotechnology
Mandalay Technological University
Myanmar
In this research, three species of beneficial bacteria Bacillus mesentericus, Nitrobacter winogradskyi and Streptococcus faecium
were isolated from different sources. Four species of beneficial bacteria Rhodopseudomonas palustris, Pseudomonas
fluorescens, Pseudomonas stutzeri and a yeast Saccharomyces cerevisiae were obtained form the Department of Biotechnology.
In this study, in vitro disease inhibition test of probiotic bacteria were tested with V. harveyi, A. hydrophila, V. parahaemolyticus
and E. coli. A new probiotic was formulated by using these seven beneficial microbes. They were inoculated in liquid carrier.
The initial bacterial population in liquid carrier was 8.8 ×10 9 CFU/ml. After three months, the bacterial population stored at
room temperature and refrigerator were 6.4×10 7 CFU/ml and 3.6×10 8 CFU/ml respectively. Six trials (i) 5ml/ton(probiotics-
P) (ii) 10ml/ton(P) (iii) 15ml/ton(P) (iv) 20ml/ton(P) (v) antibiotic (vi) without treatment with two replicate of each were
conducted as experiment I in 60 liter tanks and as experiment II in 1 ton tanks for larval rearing of giant freshwater prawn
(Macrobrachium rosenbergii). Survival rates, emergence time of post larvae, completion of post larvae development, and water
quality were found to be better in all trial with probiotic. Moreover, Vibrio counts in the larval rearing tanks were at minimum in
probiotic treatments. The microbial species diversity in hatchery tanks can be adjusted by adding selected beneficial bacteria to
displace deleterious bacteria. The formulated probiotic was found to have beneficial effect on water quality and disease control
as well as survival rates (%) in these experiments. Therefore the new formulated probiotic will be useful as biological control
and biosafety for organic aquaculture system.
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