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Table 1. Comparison of two straw types used for cryopreservation
HIGH-THROUGHPUT CRYOPRESERVATION Features IN AQUATIC CBS SPECIES: ADAPTING
MAMMALIAN TECHNOLOGY TO FISH
E Hu*, Huiping Yang and Terrence R. Tiersch
Aquaculture Research Station
Louisiana State University Agricultural Center
Baton Rouge, LA 70820 USA
ehu@agcenter.lsu.edu
TM straws French straws
Physical
high elasticity low elasticity
characteristics
identification jacket
transparent (colored
no external cover
transparent or colo
Economics
$0.51 per straw $0.04 per straw
sold in packages of 300 sold in packages o
Features of products heat sealing of both ends
0.3-ml or 0.5-ml volume
gel plugging one e
0.25-ml or 0.5-ml v
With the adoption of existing commercial technology, high-throughput
cryopreservation is possible in aquatic species. As a first step, our goal
was to adapt an automated system developed for livestock and humans
for use in channel catfish (Ictalurus punctatus). Our objectives were to:
1) compare existing and high-throughput fish sperm cryopreservation
procedures; 2) compare physical characteristics of Cryo Bio System
(CBS TM ) straws and standard French straws, and 3) begin initial research
on fish sperm for high-throughput cryopreservation.
For straw filling, sealing, and labeling, we used the CBS TM MAPI system
(www.cryobiosystem-imv.com) to provide high biosecurity and
large-scale production. There are a number of differences between the
straw types (Table 1), and the thermal properties of the straws were studied
(Figure 1). Using standard conditions, the CBS straws cooled more
slowly and required a longer thawing time. Based on our preliminary
studies for channel catfish, the cryoprotectant selected was 5% methanol,
the cooling rate was 20 ºC/min, and the thawing conditions were 40
ºC for 23 sec. More technical and economic research are necessary to
adapt the MAPI system for aquatic sperm, but it offers great potential for
culture and conservation.
Table 1. Comparison of two straw types used for cryopreservation
Features CBS TM straws French straws
Physical
high elasticity low elasticity
identification jacket no external cover
characteristics transparent (colored transparent or colored
Economics
$0.51 per straw $0.04 per straw
sold in packages of 300 sold in packages of 2000
Features of products heat sealing of both ends gel plugging one end; other end
0.3-ml or 0.5-ml volume 0.25-ml or 0.5-ml volume
Figure 1. Temperature profiles of the 0.5ml
CBS straw (dotted line) and the 0.5-ml
French straw (dashed) in response to
cooling at 40 ºC/min (top) and thawing at
40 ºC (bottom). The solid line is the
freezing chamber temperature. The
horizontal axes present time (sec); the
Figure 1. Temperature profiles of the 0.5ml
CBS straw (dotted line) and the 0.5-ml
French straw (dashed) in response to
cooling at 40 ºC/min (top) and thawing at
40 ºC (bottom). The solid line is the
freezing chamber temperature. The
horizontal axes present time (sec); the
vertical axis presents temperature (ºC).