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3<br />

AMINO ACID SUPPLEMENTATION AND DIGESTIBILITY OF PRACTICAL ORGANIC<br />

DIETS FOR NILE TILAPIA, Oreochromis niloticus, CONTAINING SOYBEAN MEAL AND<br />

COMMERCIAL YEAST EXTRACT AS TOTAL REPLACEMENTS OF FISH MEAL<br />

Kimberly A. Trosvik*, Linda S. Metts, Kenneth R. Thompson and Carl D. Webster<br />

Aquaculture Research <strong>Center</strong><br />

Kentucky <strong>State</strong> University<br />

Frankfort, KY 40601 USA<br />

kimberly.trosvik@kysu.edu<br />

Fish meal (FM) is considered the most nutritionally-complete protein source in diets for finfish due to its ideal balance of essential<br />

fatty acids, amino acids, vitamins, and minerals. Fish meal’s nutritional profile is also desirable in the livestock and poultry<br />

industries, which increases competition and demand for this resource, making fish meal the most expensive macro-ingredient<br />

of a fish diet. There are numerous attractive protein sources that potentially could replace fish meal in aquaculture diets.<br />

However, these protein sources often do not provide for similar growth in fish when compared to diets with fish meal. It has<br />

been stated that the use of two or more complimentary protein sources may allow for growth results similar to ones found when<br />

fish meal is added to a diet. Soybean meal and yeast are two favorable alternatives to fish meal that have shown some promise<br />

when used in combination for fish meal replacement.<br />

Tilapia are one of the most-cultured fin fish in the world. Organically-fed tilapia may allow producers to enter the rapidlydeveloping<br />

organic sector of the market. The objective of this study was to assess the digestibility and effect of amino acid<br />

supplementation in organic tilapia diets containing organically-certified yeast and soybean meal as a complete protein replacement<br />

for fish meal.<br />

A 6-week feeding trial was performed in a rack recirculating system comprised of 36, 10.0-L tanks stocked with 20, 200mg<br />

fish per tank. Water temperature was maintained at 28 C. Fish were fed three times daily (0800, 1200 and 1600) all they could<br />

consume in thirty minutes. Nine diets were formulated (Table 1) with 4 replicates per diet.<br />

Data will be compared between final weight, feed conversion ratio, protein efficiency ratio, and specific growth rate. Results<br />

will be discussed.<br />

Table 1. Formulation of nine experimental diets fed to Nile tilapia.<br />

Diet<br />

1 2 3 4 5 6 7 8 9<br />

Menhaden FM 20.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0<br />

NuPro© 0.0 0.0 0.0 <strong>15</strong>.0 30.0 45.0 <strong>15</strong>.0 30.0 45.0<br />

Soybean meal 50.3 84.4 83.9 67.1 51.0 35.7 68.4 51.3 33.8<br />

Lysine 0.0 0.0 0.3 0.0 0.0 0.0 0.4 0.4 0.4<br />

Methionine 0.0 0.0 0.3 0.0 0.0 0.0 0.4 0.3 0.3<br />

Other 29.7 <strong>15</strong>.6 <strong>15</strong>.5 17.9 19.0 19.3 <strong>15</strong>.8 <strong>18</strong>.0 20.5

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