February 15-18, 2009 Washington State Convention Center Seattle ...

AN ORAL DELIVERY SYSTEM FOR DNA VACCINES IN RAINBOW TROUT
Yan Zheng, John Eley, Ram Veerubotla, Sophie St-Hilaire , Marie Adomako, Wendy Sealey, Peter Sheridan,
Malcolm Shields, Brian Donahower and Scott LaPatra
Department of Pharmaceutical Sciences
Idaho State University
970 South 5th Ave.
Pocatello, ID USA
zhenyan@isu.edu
A DNA vaccine for infectious hematopoietic necrosis virus has been licensed in Canada for use in Atlantic salmon. This
vaccine is effective against the virus, but is not being investigated for use in the U.S. rainbow trout industry because of the
high cost of administering the vaccine intramuscularly. We have evaluated the use of Poly (D, L-lactide co-glycolide) polymer
(PLGA) particles to deliver DNA plasmids to fish. Particles were created which incorporated approximately 40 % of the initial
DNA. Concentrations of DNA were estimated at more than 3 ug/mg of particles, and particle size was, on average, ~600 nm.
Preliminary results from our oral and anal intubation trials suggest particles are found within the lamina propria of the lower
intestine within 24 hours with both delivery methods. Further, particles top coated on commercial trout feed pellets were also
found in the lamina propria within 48 hours.
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