February 15-18, 2009 Washington State Convention Center Seattle ...

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FEASIBILITY OF USING DIFFERENT LEVELS OF SOY PROTEIN CONCENTRATE AS
THE PROTEIN SOURCE IN FEED FOR PACIFIC WHITE SHRIMP Litopenaeus vannamei
REARED UNDER POND CONDITION
Daranee Sookying*, Jessica N. Jacquay and D. Allen Davis
Department of Fisheries and Allied Aquacultures
Auburn University
Auburn, AL 36849-5419 USA
sookyda@auburn.edu
Soybeans are one of the most widely distributed oil seeds which produce a variety of products used in the animal feed industry.
For example, solvent extracted soybean meal (SE-SBM) is one of the most efficient and widely used protein sources in the
feed industry. This is primarily due to the high quality of nutrients especially with respect to protein and amino acid profiles.
However, as compared to fish meal it has a lower nutrient content in terms of protein, essential amino acids (e.g. methionine),
essential fatty acids and minerals which can be adjusted with supplements or blending with other products. As the nutrient
density (protein and energy) of the diet is increased the use of SE-SBM is inefficient as there is insufficient room in the formulation
for all necessary components. Furthermore, even in diet with moderate levels of protein there are advantages to having a
high protein ingredient in the formulation. Soy protein concentrate (SPC) is a high protein ingredient that is produced through
a series of different extraction and precipitation process from high quality dehulled soybeans. By removing most of the oil and
water soluble non-protein constituents a variety of high protein products with varying characteristics can be produced. These
products are increasingly being used by the animal feed industry. As the availability of these product increases, the price is
likely to be reduced making them cost effective solutions. As SPC has a higher protein content than SE-SBM, there is interest
in using these meals in practical diet formulations. The objective of this project was to demonstrate the feasibility of diets
formulated to contain increasing percentages of SPC (0%, 4%, 8%, and 12%), in production diets for L. vannamei reared under
pond production conditions. The research was conducted at Claude Peteet Mariculture Center in Gulf Shores, Alabama. Four
diets are currently being tested in sixteen 0.1 Ha ponds using four replicates per diet. Post larvae were obtained from Shrimp
Improvement Systems in the Florida Keys, Florida and nursed for two weeks, juvenile shrimps (~0.01g) were stocked at 35
shrimp/m 2 and will be cultured under standardized production conditions. Feed inputs are pre-scheduled using feeding protocols
that have maximum feed inputs of 7.23 kg/ha/day. The experiment is currently ongoing and the growout stage is scheduled
to last 17 weeks. After 9 weeks of pond culture, cast net samples have an average mean weight of 10.41 grams, and all treatments
are showing similar growth rates. Final yield, weight gain, survival rate, and feed conversion ratio will be determined
and reported at the conclusion of the production cycle of the ponds after harvest. Moreover, the same diets were tested in 20
tanks (800L) with five replications per treatment, and four tanks (800L) were used with a fifth diet that served as a commercial
reference with four replicates. Juvenile shrimp (1.0g initial weight) were stocked at a density of 30 shrimp per tank. Water
was pumped from a production pond to mimic production pond conditions and water exchanges occurred daily throughout the
12 week trial. Daily feed inputs were calculated, based upon an expected weight gain of 1.3 g per week and an expected feed
conversion ratio of 1.2:1. Growth, survival, and feed conversion ratio will be determined at the end of experimental period.