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February 15-18, 2009 Washington State Convention Center Seattle ...

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2<br />

ORGANIC CARBON AND NITROGEN REMOVAL FROM GARFISH AQUACULTURE<br />

USING SEQUENTIAL BATCH REACTOR - A LABORATORY SCALE STUDY<br />

Dhritikshama Roy* and Raj Boopathy<br />

Department of Biology<br />

Nicholls <strong>State</strong> University<br />

Thibodaux, LA 70310 USA<br />

Royd425@its.nicholls.edu<br />

Garfish is a fresh water fish that can tolerate moderate range of salinity. Research is underway at Nicholls <strong>State</strong> University to<br />

grow garfish under various salinity levels because of the availability of saline water. Aquaculture of garfish is in experimental<br />

stage in Louisiana. The experimental aquaculture system involves feeding garfish with 40% protein diet. This higher protein<br />

diet resulted in the accumulation of high concentration of dissolved organic carbon (~ 6500mg/L), ammonia-N (~ 600 mg/L),<br />

nitrite-N (~ 400 mg/L)) and nitrate (~650 mg/L)) in garfish aquaculture system.<br />

The objective of this study was to treat the garfish aquaculture waste water using Sequential Batch Reactor (SBR). Sequential<br />

batch reactor (SBR) is an activated sludge treatment with a combination of variable period of aerobic and anaerobic modes.<br />

The experiment used garfish waste water of three different concentrations of salt – 4.0 ppt, 8.0 ppt and 12.0 ppt. Garfish waste<br />

without salt served as control. Performance of SBR showed significant removal of dissolved organic carbon and total nitrogen<br />

within <strong>15</strong> days with 11 days of aerobic condition followed by 2 days of anaerobic and 2 days of aerobic modes for various<br />

salinity garfish waste water. The removal rate was concentration dependent. The quantitative effect of heterotrophic bacteria in<br />

garfish aquaculture was also noticeable during waste removal process through SBR.

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