February 15-18, 2009 Washington State Convention Center Seattle ...

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EVALUATION OF DIETS CONTAINING SOYBEAN MEAL, WITH AND WITHOUT AMINO
ACID SUPPLEMENTATION, AS TOTAL REPLACEMENT FOR FISH MEAL FOR JUVENILE
NILE TILAPIA, Oreochromis niloticus
Linda S. Metts*, Kenneth R. Thompson and Carl D. Webster
Aquaculture Research Center
Kentucky State University
Frankfort, KY 40601 USA
linda.metts@kysu.edu
Global production of tilapia has increased dramatically over the past few years due to new technologies and research that have
allowed for expansion of aquaculture production. World-wide tilapia production is expected to reach 4 million tonnes in the
near future. However, with the use of fish meal in aquaculture diets being questioned by some as either environmentally and/or
financially unsound, the need for partial or total replacement of fish meal with plant and animal alternatives is essential for
the future growth of the aquaculture industry. Therefore, the objective of the study was to evaluate the use of a less-expensive
protein source (soybean meal), with and without amino acid supplementation, as total replacement of fish meal (FM) in diets
for Nile tilapia (Oreochromis niloticus).
An 8-week feeding trial will be conducted on small (100-200 mg) Nile tilapia obtained from a commercial supplier. Nile tilapia
will be fed nine experimental diets, with eight experimental diets formulated to contain either all soybean meal (with and
without methionine and/or lysine supplementation), or a blend of soybean meal and either poultry by-product meal or distillers
dried grains with solubles. The ninth diet will be formulated to be similar to a high-quality commercial tilapia diet which will
contain 20% menhaden fish meal and 40% protein. Weight gain, survival, and body composition will be measured at the conclusion
of each 8-week feeding trial. Since the future of long-term availability of FM is uncertain, and since FM is one of the
most expensive ingredients in an aquaculture diet, it is important to the aquaculture industry that production diets be formulated
using alternative ingredients that can replace or reduce the percentages of FM without adversely affecting growth and health of
fish. The results of this study may give new insights on possible alternative protein sources that can replace FM in Nile tilapia
diets as well as other omnivorous and/or carnivorous species, which may allow further expansion of the aquaculture industry.
COMPARISON OF MIST AND MODIFIED-MIST PROCEDURES FOR QUICK EGG
REMOVAL FROM OVULATED PADDLEFISH
Steven D. Mims*, Richard J. Onders and Boris Gomelsky
Aquaculture Research Center
Kentucky State University
Frankfort, KY 40601 USA
steven.mims@kysu.edu
A procedure known as Minimally Invasive Surgical Technique (MIST) has been successfully evaluated and practiced for quick
egg removal from ovulated paddlefish. Though the technique requires little out-of water handling time for the fish and results
in high egg yields and broodstock survival, a modified MIST will be presented to show an alternative procedure that is as safe
for the brood stock but even quicker in egg removal. Embedded video will be used to illustrate and to compare between the
two MIST procedures.