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A biological study of Durvillaea antarctica (Chamisso) Hariot and D ...

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2.2<br />

ANJ\ LY~ I ~;<br />

4<br />

(a)<br />

Samples used for population analyses are listed in Appendix S.<br />

Unless otherwise stated, all plants, including those just visible<br />

to the naked eye, were collected. In most cases the samples came<br />

from a known area <strong>of</strong> shore <strong>and</strong> extended across the depth <strong>of</strong> the kelp<br />

bond. Samples were effectively belt. h::ansects I the width <strong>of</strong>: IrJhich<br />

depended on the accessibility <strong>of</strong> the area, shore slope <strong>and</strong> sea<br />

conditions duriHgthe sampling period.<br />

Measurements <strong>of</strong> individual plants included total length, stipe<br />

length, stipe din.meter' (a t the base <strong>of</strong> the stipe), holdfast diameter<br />

(for specimens with 50litaxy holdfasts), maximum lamina ·thickness,<br />

total weight (!,,'et) <strong>and</strong> stipe weight (Fig. 2.4). Subjective measurements<br />

included the degree <strong>of</strong> division <strong>of</strong> laminae, the degree <strong>of</strong><br />

honeycombing (taking into account both thickness <strong>and</strong> the expanse<br />

honeycombed) , the area <strong>of</strong> lamina covered with t.onceptacles <strong>and</strong> the<br />

extent <strong>of</strong> burrowing' in the holdfast. 'l'here were seven ascending<br />

catego:cies: nil, low, I oVI/medium, medium, medium/high, high <strong>and</strong><br />

V0Dj high.<br />

Subsamples were selected by laying all specimens out in rows<br />

on a beach or rock platform <strong>and</strong> :c<strong>and</strong>omly selecting plants, having<br />

previously prepared a list <strong>of</strong> r<strong>and</strong>om numbers cr:canged in ascending<br />

order.<br />

Seasonal changes in population structure were monitored at<br />

Tautuku because all samples were exposed to similar cortcli lions"<br />

To reduce as fa.r as possible any influence <strong>of</strong> sea,son, specimens<br />

shorter than 1 m we.t"e excluded from analyses when comparing samples<br />

from ~'lidely<br />

sepay'ated arEas.<br />

(b)<br />

A fragIllent <strong>of</strong> lamina <strong>of</strong> each fertile plant was collected in all<br />

samples. These were preserved in 10% seawater formalin <strong>and</strong> later<br />

examined in the laboratory to assess their reproductive state.<br />

Samples <strong>of</strong> the blade \

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