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A biological study of Durvillaea antarctica (Chamisso) Hariot and D ...

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141<br />

A large number <strong>of</strong> ova at 20°C disintegrated shortly after they h'ere<br />

released, whereas this was relatively uncommon at the lower<br />

temperatures. After 120 minutes, a small piece <strong>of</strong> ripe male lamina<br />

was added to each dish, <strong>and</strong> all 30 dishes were kept at 10-ll~e in a<br />

Two days later the oosphere3 were examined.<br />

Almost all the oospheres in the 20°C dishes had disintegrated, <strong>and</strong><br />

none appeared to be germil~ting. A large proportion <strong>of</strong> the oospheres<br />

in the SoC <strong>and</strong> 11°C dishes however, looked perfectly healthy, <strong>and</strong><br />

were germinating.<br />

Table 6.3<br />

Subjective rating <strong>of</strong> gamete release at three temperatures.<br />

Number <strong>of</strong> samples used in each treatment '= 10.<br />

(a)<br />

Nl..l1nber rated as "high" or "medium" release:<br />

Species<br />

Temperature<br />

11°C<br />

D. <strong>antarctica</strong><br />

6<br />

9<br />

1<br />

D. willana<br />

4<br />

10<br />

o<br />

(b)<br />

Number rated as "very 10'."" or "nil" release:<br />

D. <strong>antarctica</strong><br />

1<br />

o<br />

S<br />

D. wiUana<br />

3<br />

o<br />

10<br />

Table 6.4 Frequency <strong>of</strong> D. <strong>antarctica</strong> gametes released at three<br />

temperatures. Mea.n number <strong>of</strong> ova counted using line intercept<br />

method.<br />

Sample number<br />

Temperature<br />

SOC<br />

lloe<br />

20 0 e<br />

1 2100<br />

:2 8.1<br />

3 38.6<br />

4 2.7<br />

5 13.9<br />

6 75.3<br />

7 5.8<br />

8 40.J.<br />

9 20.5<br />

10 8.5<br />

23.5 ±<br />

35.0 1.3<br />

32.0 1.5<br />

14.8 8.0<br />

20.0 4.0<br />

21. 3 9.0<br />

52.3 18.9<br />

40.0 4.5<br />

71.0 28.3<br />

21.1 17 .8<br />

9./l 3 .. 6<br />

3L7 ± 9,.6 1:

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