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SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

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Ch. 5 – Preparing to Take a PictureTo use the Correct Color feature in <strong>Advanced</strong> Mode, simply make sure the checkbox is checked in theimage setup window (under post processing) <strong>and</strong> then in the live image controls. In <strong>Basic</strong> Mode, simplycheck the checkbox in the image settings window. In TWAIN or Mac 3 rd party mode, check the checkbox<strong>for</strong> Correct Color.In order to get the best results using this feature, you should make sure you set up your imaging systemincluding your computer monitor appropriately. See Appendix F <strong>for</strong> more in<strong>for</strong>mation on system set-up.Be aware that the color correction trans<strong>for</strong>mations that occur when you use Correct Color Technology willbe applied to the raw image data, leaving a modified image file. If you will require the raw data <strong>for</strong> furtheranalysis of your image, you should uncheck the Correct Color checkbox.Chip Defect CorrectionWhen you capture an image, the <strong>SPOT</strong> program automatically compensates <strong>for</strong> CCD chip defects, so thatthey do not appear on your images. The Chip Defect Correction option allows you to turn off this automaticcorrection. This can be useful in cases when you are per<strong>for</strong>ming quantitative measurements of pixelbrightness values <strong>and</strong> need to see precisely where chip defects are.The Chip Defect Correction box is, as a default, checked when you define a new image setup. To turn offthe program's automatic chip correction, deselect the Chip Defect Correction box.Noise FilterThe Noise Filter corrects <strong>for</strong> the effects of electrical or thermal noise. When capturing dim images withlengthy exposure times, the noise filter acts as a “virtual cooler” <strong>for</strong> the <strong>Insight</strong> camera, eliminating “hotpixels.”The Noise Filter checks each pixel on the CCD chip as described below:1. The program compares the value of each red pixel with the values of the eight neighboring red pixels.2. If none of the eight neighboring red pixels has a value within ±50 % (i.e., the threshold value that the<strong>SPOT</strong> program uses) of the value <strong>for</strong> the red pixel being checked, the value <strong>for</strong> the red pixel beingchecked is replaced with the average value of the eight neighboring red pixels.3. The program repeats this procedure <strong>for</strong> the values of the green <strong>and</strong> blue pixels being checked.To use the Noise Filter, follow these steps:To filter noise, follow these steps:1. Check the Noise Filter box.2. Enter a value between 10% <strong>and</strong> 100%. The Threshold value is set to a default of 50%. Refer to theprocedure described above <strong>for</strong> an explanation of the Threshold value.3. When you capture an image, the program automatically adjusts the image using the Noise Filter valuethat you entered.Note: You can also use the Filter Noise Edit menu option to adjust captured images, asdescribed in Ch. 8 – Viewing, Editing, <strong>and</strong> Annotating Images.Gamma AdjustGamma Adjust smoothly lightens darker areas of an image without burning out bright areas or lighteningblack areas. This is especially useful <strong>for</strong> capturing/editing images with a wide range of brightness values(dynamic range) such as:• Fluorescence specimens• Macroscopic objects with glints, such as printed circuitryUser Guide to the <strong>SPOT</strong> <strong>Insight</strong> Camera 107

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