SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

Ch. 5 – Preparing to Take a PictureIf you select the Add option (i.e. to create a new imagesetup), the New Image Setup window appears. The predefinedtemplates (image types) are indicated by brackets< >, while user-defined setups appear without brackets.InsightB/WInsight B/W and Color Mosaic camera userswill not see the fluorescence – red,fluorescence – green, and fluorescence –blue Image Type options.1. Select an image type from the list.The 11 pre-defined image types (in addition to theFactory Defaults setting) correspond to commonly usedmicroscopy techniques. To define a new image setup,you must also select an image type as a template fordefining the new setup. The image type provides a baseline set of values for that setup, according tothe microscopy technique you are using. The following table describes SPOT’s pre-defined imagetypes:Image TypeBrightfield-transmitted lightDarkfield-transmitted lightFluorescenceFluorescence – redFluorescence – greenFluorescence – blueNomarski DIC/HoffmanPhase contrastPolarized lightBrightfield-reflected lightDarkfield-reflected lightStereo/macro–no glintsStereo/macro–shiny glintsStereo/macro-metallic glintsDescriptionStained specimens on glass slides using transmitted light microscopes.Bright specimens on dark backgrounds using transmitted light darkfieldtechnique.Fluorescence specimens not fluorescing in a single red, green, or blueprimary color. The camera exposes images using all three colors.Fluorescence specimens fluorescing in red. Exposure time is reducedbecause only one color is exposed.Fluorescence specimens fluorescing in green. Exposure time isreduced because only one color is exposed.Fluorescence specimens fluorescing in blue. Exposure time is reducedbecause only one color is exposed.Specimens being viewed using Nomarski DIC or Hoffman techniques.Specimens being viewed using the phase contrast technique.Specimens being viewed with a polarized light microscope.Polished metallurgical specimens and semi-conductors using amicroscope with a vertical illuminator.Bright areas of specimens being viewed with darkfield, reflected lighttechniques.Matte specimens without bright glints.Shiny specimens with bright glints.Metallic specimens with intense glints.User Guide to the SPOT Insight Camera 89