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SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

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Ch. 8 – Viewing, Editing, <strong>and</strong> Annotating Images6. Size - Enter the size of the calibration mark. You can modify the size either by entering the number inthe Size box, or by using the cursor to stretch the on-screen calibration bar. To adjust the size of thecalibration mark, move the cursor to the end of the calibration bar line. The st<strong>and</strong>ard cursor changes toa two-headed arrow. Drag the end of the bar to adjust the length. The calibration mark numberchanges to reflect the new length.7. Click on Stamp to incorporate the calibration mark into the image.Add MeasurementThe Add Measurement option enables you to incorporate measurements into an image capture, includingline, area, <strong>and</strong> angle measurements.To add a measurement, follow these steps:1. If you have not already calibrated the objectives on yourmicroscope, do so. Refer to Ch. 4 – The <strong>Advanced</strong> ProgramMode <strong>for</strong> details.2. From the Edit menu, select Add Measurement. TheMeasurement window appears.3. From the Calibration Setup drop-down list, select the setup(e.g., 15x) <strong>for</strong> the objective that you are using.4. Check the type of measurement that you want.−−−−−−−−Straight Line – Point to point distance measurementCurve Length – Length of a freeh<strong>and</strong> lineRectangle Perimeter – Length around the outside edgeof a rectangleEllipse Perimeter – Length around the outside of aperimeterRegion Area – Area of a freeh<strong>and</strong> outlineRectangle Area – Area of a rectangular shapeEllipse Area – Area of an elliptical shapeAngle – Angle measurement5. Move the cross-hair cursor to the point in the image capture where you want to begin the measurement.6. Single click to activate the drawing function. Do not hold the mouse button down between themeasurement points.−−−−For a Straight Line, move the cross-hair cursor to the end point of the measurement. Single clickto deactivate the drawing function <strong>and</strong> display the length of the line.For a Curve, use the cross-hair cursor to trace a freeh<strong>and</strong> line on an image. Single click todeactivate the drawing function <strong>and</strong> display the length of the freeh<strong>and</strong> line.For an Angle, use the cross-hair cursor to specify the three points that define the angle. Singleclick to mark the end point of the first side. Move the cross hairs to the vertex, <strong>and</strong> single clickagain. Move the cross hairs to the end point of the second side, <strong>and</strong> single click. This last clickdeactivates the drawing function <strong>and</strong> displays the angle measurement.For a Region Area, use the cross-hair cursor to trace a freeh<strong>and</strong> outline around the area that youwant to measure. Single click to deactivate the drawing function <strong>and</strong> display the measurement. If162 User Guide to the <strong>SPOT</strong> <strong>Insight</strong> Camera

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