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SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

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Ch. 7 - Taking a PictureIntroduction: Three Ways to Take a PictureThere are three ways to take a picture with the <strong>SPOT</strong> camera:• Live Image Pause/TransferAfter pausing a live image, pressing the Transfer button saves the image to a window. This is useful<strong>for</strong> capturing rapidly changing samples. Images captured in this way are essentially “frozen” liveimages. As such, the right <strong>and</strong> left sides of the images are read out via separate amplifiers <strong>and</strong> arelimited to 8 bit monochrome/24 bit color.• SnapPressing the Snap button when viewing a live image initiates an image capture where the entire imageis read out via a single amplifier. Snap image captures avoid the time dedicated to computing exposureby using the exposure time <strong>and</strong> gain calculated <strong>for</strong> the live image.• Get Image (Toolbar button)Pressing the Get Image toolbar button initiates an image capture where the entire image is read out viaa single amplifier using only the parameters from the current image setup. This allows you to use thelive image window purely as an aid to framing <strong>and</strong> focusing the image. You may also access GetImage from the dropdown menu (under Camera), from the toolbar, or by creating <strong>and</strong> using a Taskbarbutton (Chapter 12) <strong>for</strong> Get Image Adjustments made to the live image have no impact on the imagecapture.These three types of image captures were designed to give you greater flexibility in working with differenttypes of specimens <strong>and</strong> in how you use live images.• For more details on the Live Image Pause/Transfer <strong>and</strong> Snap options, refer to Ch. 6 – Using LiveImages.• For more details on the Get Image option, refer to the Get Image (exposure from Setup) sectionbelow.This chapter discusses the Get Image type of capture as used in a variety of different procedures. Thechapter is divided into two sections:• <strong>Basic</strong> Image Captures –St<strong>and</strong>ard <strong>and</strong> sequential image captures.• Image Captures in Other Procedures – Image captures where one capture is used to prepare <strong>for</strong>another capture.<strong>Basic</strong> Image CapturesThe <strong>SPOT</strong> software offers three primary image capture options:• Get Image (exposure from setup)• Get Image (last used exposure)• Get Sequential Images122 User Guide to the <strong>SPOT</strong> <strong>Insight</strong> Camera

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